NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW;
DOI=10.1016/0378-1119(90)90342-O; PubMed=2265761 [NCBI, ExPASy, EBI, Israel, Japan]
Menon A.L., Stults L.W., Robson R.L., Mortenson L.E.;
"Cloning, sequencing and characterization of the [NiFe]hydrogenase-encoding structural genes (hoxK and hoxG) from Azotobacter vinelandii.";
Gene 96:67-74(1990).

Comments

FUNCTION: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.
CATALYTIC ACTIVITY: H₂ + A = AH₂.
COFACTOR: Binds 1 nickel ion per subunit (By similarity).
SUBUNIT: Heterodimer of a large and a small subunit.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
PTM: 15 or 16 residues are removed from the C-terminus during maturation of the protein.
SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M33152; AAA82506.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L23970; AAA19499.1; -; Unassigned_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

JQ0806; JQ0806.

3D structure databases

HSSP

P12944; 2FRV. [HSSP ENTRY / PDB]

ModBase

P21949.

Ontologies

GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0033748;	Molecular function: hydrogenase (acceptor) activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001501; Ni-dep_hyd_lsu.
Graphical view of domain structure.

Pfam

PF00374; NiFeSe_Hases; 1.
Pfam graphical view of domain structure.

PROSITE

PS00507; NI_HGENASE_L_1; 1.
PS00508; NI_HGENASE_L_2; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Membrane; Metal-binding; Nickel; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 602`	`602`	`Uptake hydrogenase large subunit.`	`PRO_0000199709`
`METAL`	`74 74`		`Nickel (Potential).`
`METAL`	`77 77`		`Nickel (Potential).`
`METAL`	`581 581`		`Nickel (Potential).`
`METAL`	`584 584`		`Nickel (Potential).`

Sequence information

Length: 602 AA [This is the length of the unprocessed precursor]

Molecular weight: 66737 Da [This is the MW of the unprocessed precursor]

CRC64: BCC64BB2F12B3240 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSSLPNASQL DKSGRRIVVD PVTRIEGHMR CEVNVDASNV ITNAVSTGTM WRGLEVILKG 

        70         80         90        100        110        120 
RDPRDAWAFV ERICGVCTGT HALTSVRAVE DALDIRIPYN AHLIRNLMDK TLQVHDHIVH 

       130        140        150        160        170        180 
FYHLHALDWV NPVNALKADP KATSALQQAV SPAHAKSSPG YFRDVQTRLK KFVESGQLGL 

       190        200        210        220        230        240 
FSNGYWDNPA YKLPPEADLM AVAHYLEALD LQKDIVKIHT IFGGKNPHPN YMVGGVACAI 

       250        260        270        280        290        300 
NLDDVGAAGA PVNMTSLNFV LERIHEAREF TRNVYLPDVL AVAGIYKDWL YGGGLAAHNL 

       310        320        330        340        350        360 
LSYGTFTKVP YDKSSDLLPA GAIVGGNWDE VLPVDVRDPE EIQEFVSHSW YSYADETKGL 

       370        380        390        400        410        420 
HPWDGVTEPK FELGPNTKGS RTHIQEIDEA HKYSWIKAPR WRGHAMEVGP LARYIIAYAS 

       430        440        450        460        470        480 
GREYVKEQVD RSLAAFNQST GLNLGLKQFL PSTLGRTLAR ALECELAVDS MLDDWQALVG 

       490        500        510        520        530        540 
NIKAGDRATA NVEKWDPSTW PKEAKGVGIN EAPRGALGHW IRIKDGKIEN YQAIVPTTWN 

       550        560        570        580        590        600 
GTPRDHLGNI GAYEAALLNT RMERPDEPVE ILRTLHSFDP CLACSTHVMS PDGQELTRVK 


VR

P21949 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools