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UniProtKB/Swiss-Prot entry P20788


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name UCRI_RAT
Primary accession number P20788
Secondary accession number Q6QI13
Integrated into Swiss-Prot on February 1, 1991
Sequence was last modified on September 13, 2004 (Sequence version 2)
Annotations were last modified on    September 2, 2008 (Entry version 72)
Name and origin of the protein
Protein name Cytochrome b-c1 complex subunit Rieske, mitochondrial [Precursor]
Synonyms EC 1.10.2.2
Ubiquinol-cytochrome c reductase iron-sulfur subunit
Rieske iron-sulfur protein
RISP
Complex III subunit 5
Liver regeneration-related protein LRRGT00195
Contains Cytochrome b-c1 complex subunit 11
     (Ubiquinol-cytochrome c reductase 8 kDa protein)
     (Complex III subunit IX)
Gene name
Name: Uqcrfs1
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Xu C.S., Zhang L., Chang C.F., Han H.P., Wang G.P., Chai L.Q., Yuan J.Y., Yang K.J., Zhao L.F., Ma H., Wang L., Wang S.F., Xing X.K., Shen G.M., Shi J.B., Rahman S., Wang Q.N., Zhang J.B.;
"Liver regeneration after PH.";
Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Ovary;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 19-274.
DOI=10.1016/0006-291X(89)92398-X; PubMed=2538119 [NCBI, ExPASy, EBI, Israel, Japan]
Nishikimi M., Hosokawa Y., Toda H., Suzuki H., Ozawa T.;
"Cloning and sequence analysis of a cDNA encoding the Rieske iron-sulfur protein of rat mitochondrial cytochrome bc1 complex.";
Biochem. Biophys. Res. Commun. 159:19-25(1989).
[4]
PROTEIN SEQUENCE OF 85-92 AND 156-163, AND MASS SPECTROMETRY.
STRAIN=Sprague-Dawley;
TISSUE=Hippocampus;
Lubec G., Chen W.-Q.;
Submitted (APR-2007) to UniProtKB.
Comments
  • FUNCTION: Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
  • FUNCTION: The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex (By similarity).
  • CATALYTIC ACTIVITY: QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
  • COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).
  • SUBUNIT: The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1) (By similarity).
  • SUBCELLULAR LOCATION: Mitochondrion inner membrane.
  • MISCELLANEOUS: The Rieske protein is a high potential 2Fe-2S protein.
  • SIMILARITY: Contains 1 Rieske domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AY539946; AAS66286.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC085339; AAH85339.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M24542; AAA42051.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A32296; A32296.
RefSeq NP_001008888.1; -.
UniGene Rn.2603
3D structure databases
HSSP P13272; 1RIE. [HSSP ENTRY / PDB]
SMR P20788; 1-57, 79-274.
ModBase P20788.
Protein-protein interaction databases
IntAct P20788; -.
2D gel databases
Rat-heart-2DPAGE P20788; -.
Organism-specific databases
RGD 628838; Uqcrfs1.
Gene expression databases
ArrayExpress P20788; -.
GermOnline ENSRNOG00000018281; Rattus norvegicus.
Family and domain databases
InterPro IPR014349; Rieske.
IPR005805; Rieske_C.
IPR006317; Rieske_proteo.
IPR005806; Rieske_reg.
IPR015248; Ubiq_cytc_red_N.
IPR004192; UCR_TM_region.
Graphical view of domain structure.
Gene3D G3DSA:2.102.10.10; Rieske_reg; 1.
PANTHER PTHR10134; Rieske; 1.
Pfam PF00355; Rieske; 1.
PF09165; Ubiq-Cytc-red_N; 1.
PF02921; UCR_TM; 1.
Pfam graphical view of domain structure.
PRINTS PR00162; RIESKE.
TIGRFAMs TIGR01416; Rieske_proteo; 1.
PROSITE PS51296; RIESKE; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P20788.
Genome annotation databases
Ensembl ENSRNOG00000018281; Rattus norvegicus. [Contig view]
GeneID 291103; -.
KEGG rno:291103; -.
NMPDR fig|10116.3.peg.13264; -.
Phylogenomic databases
HOVERGEN P20788; -.
Other
ProtoNet P20788.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
2Fe-2S; Direct protein sequencing; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Respiratory chain; Transit peptide; Transmembrane; Transport.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1    78  78     Cytochrome b-c1 complex subunit 11 (By similarity). PRO_0000307248
TRANSIT   1    78  78     Mitochondrion (By similarity). 
CHAIN   79   274  196     Cytochrome b-c1 complex subunit Rieske (By similarity). PRO_0000030671
DOMAIN   187   272  86     Rieske. 
METAL   217   217        Iron-sulfur (2Fe-2S) (By similarity). 
METAL   219   219        Iron-sulfur (2Fe-2S); via pros nitrogen (By similarity). 
METAL   236   236        Iron-sulfur (2Fe-2S) (By similarity). 
METAL   239   239        Iron-sulfur (2Fe-2S); via pros nitrogen (By similarity). 
DISULFID   222   238        By similarity. 
Sequence information
Length: 274 AA [This is the length of the unprocessed precursor] Molecular weight: 29446 Da [This is the MW of the unprocessed precursor] CRC64: 6A0E0A07E956340B [This is a checksum on the sequence]
        10         20         30         40         50         60 
MLSVAARSGP FAPVLSATSR GVAGALRPLL QSAVPATSEP PVLDVKRPFL CRESLSGQAA 

        70         80         90        100        110        120 
TRPLVATVGL NVPASVRYSH TDIKVPDFSD YRRAEVLDST KSSKESSEAR KGFSYLVTAT 

       130        140        150        160        170        180 
TTVGVAYAAK NAVSQFVSSM SASADVLAMS KIEIKLSDIP EGKNMAFKWR GKPLFVRHRT 

       190        200        210        220        230        240 
KKEIDQEAAV EVSQLRDPQH DLERVKKPEW VILIGVCTHL GCVPIANAGD FGGYYCPCHG 

       250        260        270 
SHYDASGRIR KGPAPLNLEV PTYEFTSGDV VVVG 

P20788 in FASTA format

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