[1]	PROTEIN SEQUENCE. DOI=10.1016/0014-5793(90)81504-H; PubMed=2194840 [NCBI, ExPASy, EBI, Israel, Japan] Marekov L., Krook M., Joernvall H.; "Prokaryotic 20 beta-hydroxysteroid dehydrogenase is an enzyme of the 'short-chain, non-metalloenzyme' alcohol dehydrogenase type."; FEBS Lett. 266:51-54(1990).
[2]	X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS). PubMed=1946424 [NCBI, ExPASy, EBI, Israel, Japan] Ghosh D., Weeks C.M., Grochulski P., Duax W.L., Erman M., Rimsay R.L., Orr J.C.; "Three-dimensional structure of holo 3 alpha,20 beta-hydroxysteroid dehydrogenase: a member of a short-chain dehydrogenase family."; Proc. Natl. Acad. Sci. U.S.A. 88:10064-10068(1991).

Comments

CATALYTIC ACTIVITY: Androstan-3-alpha,17-beta-diol + NAD⁺ = 17-beta-hydroxyandrostan-3-one + NADH.
PATHWAY: Lipid metabolism; C21-steroid hormone metabolism .
SUBUNIT: Homotetramer.
SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

PIR

S10707; S10707.

3D structure databases

PDB

1HDC; X-ray; 2.20 A; A/B/C/D=2-255.	[ExPASy / RCSB / EBI]
2HSD; X-ray; 2.64 A; A/B/C/D=2-255.	[ExPASy / RCSB / EBI]

Detailed list of linked structures.

PDBsum

1HDC; -.
2HSD; -.

ModBase

P19992.

Family and domain databases

InterPro

IPR002198; DHase_sc/Rdtase_SDR.
IPR002347; Glc/ribitol_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR19410; ADH_short_C2; 1.

Pfam

PF00106; adh_short; 1.
Pfam graphical view of domain structure.

PRINTS

PR00081; GDHRDH.
PR00080; SDRFAMILY.

PROSITE

PS00061; ADH_SHORT; 1.

Other

P19992; -.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Direct protein sequencing; Lipid metabolism; NAD; Oxidoreductase; Steroid metabolism.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 255`	`255`	`3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase.`	`PRO_0000054713`
`NP_BIND`	`10 34`	`25`	`NAD.`
`ACT_SITE`	`152 152`		`Proton acceptor.`
`BINDING`	`139 139`		`Substrate (By similarity).`
`STRAND`	`7 12`	`6`
`TURN`	`13 15`	`3`
`HELIX`	`17 28`	`12`
`STRAND`	`32 38`	`7`
`HELIX`	`40 49`	`10`
`HELIX`	`51 53`	`3`
`STRAND`	`54 58`	`5`
`HELIX`	`64 78`	`15`
`STRAND`	`83 86`	`4`
`HELIX`	`96 98`	`3`
`HELIX`	`101 111`	`11`
`HELIX`	`113 128`	`16`
`STRAND`	`132 137`	`6`
`HELIX`	`140 142`	`3`
`HELIX`	`150 170`	`21`
`HELIX`	`171 173`	`3`
`STRAND`	`175 182`	`8`
`HELIX`	`188 191`	`4`
`TURN`	`192 194`	`3`
`HELIX`	`214 225`	`12`
`HELIX`	`227 229`	`3`
`STRAND`	`236 240`	`5`
`TURN`	`241 245`	`5`
`HELIX`	`249 254`	`6`

Sequence information

Length: 255 AA [This is the length of the unprocessed precursor]

Molecular weight: 26484 Da [This is the MW of the unprocessed precursor]

CRC64: 9CB93CB66AA628D5 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNDLSGKTVI ITGGARGLGA EAARQAVAAG ARVVLADVLD EEGAATAREL GDAARYQHLD 

        70         80         90        100        110        120 
VTIEEDWQRV VAYAREEFGS VDGLVNNAGI STGMFLETES VERFRKVVDI NLTGVFIGMK 

       130        140        150        160        170        180 
TVIPAMKDAG GGSIVNISSA AGLMGLALTS SYGASKWGVR GLSKLAAVEL GTDRIRVNSV 

       190        200        210        220        230        240 
HPGMTYTPMT AETGIRQGEG NYPNTPMGRV GNEPGEIAGA VVKLLSDTSS YVTGAELAVD 

       250 
GGWTTGPTVK YVMGQ

P19992 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools