ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry P19499


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name FRHB_METTH
Primary accession number P19499
Secondary accession number O27356
Integrated into Swiss-Prot on February 1, 1991
Sequence was last modified on January 23, 2007 (Sequence version 4)
Annotations were last modified on    September 2, 2008 (Entry version 57)
Name and origin of the protein
Protein name Coenzyme F420 hydrogenase subunit beta
Synonyms EC 1.12.98.1
8-hydroxy-5-deazaflavin-reducing hydrogenase subunit beta
FRH
Gene name
Name: frhB
OrderedLocusNames: MTH_1297
From
Methanobacterium thermoautotrophicum [TaxID: 187420] [HAMAP proteome]
Taxonomy Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-31 AND 185-201.
STRAIN=Delta H;
DOI=10.1021/bi00483a011; PubMed=2207102 [NCBI, ExPASy, EBI, Israel, Japan]
Alex L.A., Reeve J.N., Orme-Johnson W.H., Walsh C.T.;
"Cloning, sequence determination, and expression of the genes encoding the subunits of the nickel-containing 8-hydroxy-5-deazaflavin reducing hydrogenase from Methanobacterium thermoautotrophicum delta H.";
Biochemistry 29:7237-7244(1990).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Delta H;
PubMed=9371463 [NCBI, ExPASy, EBI, Israel, Japan]
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R., Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.;
"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics.";
J. Bacteriol. 179:7135-7155(1997).
[3]
PROTEIN SEQUENCE OF 2-32.
STRAIN=Delta H;
DOI=10.1021/bi00388a007; PubMed=3663585 [NCBI, ExPASy, EBI, Israel, Japan]
Fox J.A., Livingston D.J., Orme-Johnson W.H., Walsh C.T.;
"8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization.";
Biochemistry 26:4219-4227(1987).
Comments
  • FUNCTION: Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.
  • CATALYTIC ACTIVITY: H2 + coenzyme F420 = reduced coenzyme F420.
  • COFACTOR: Nickel.
  • COFACTOR: Iron-sulfur. There are 12-13 Fe atoms per alpha/beta/gamma unit of the FRH.
  • COFACTOR: FAD.
  • SUBUNIT: Heterocomplex of the form (alpha(1)beta(1)gamma(1))(8).
  • SIMILARITY: Belongs to the frhB family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
J02914; AAA72190.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE000666; AAB85777.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR D35620; D35620.
RefSeq NP_276416.1; -.
3D structure databases
ModBase P19499.
Enzyme and pathway databases
BioCyc MTHE187420:MTH1297-MON; -.
Family and domain databases
InterPro IPR007516; Co_F420_Hydgase/DHase_bsu_N.
IPR017679; Coenz_F4210_hydrogenase_bsu.
IPR007525; FrhB_FdhB_C.
Graphical view of domain structure.
Pfam PF04432; FrhB_FdhB_C; 1.
PF04422; FrhB_FdhB_N; 1.
Pfam graphical view of domain structure.
BLOCKS P19499.
Genome annotation databases
GeneID 1471014; -.
GenomeReviews AE000666_GR; MTH_1297.
KEGG mth:MTH1297; -.
NMPDR fig|187420.1.peg.1271; -.
Phylogenomic databases
HOGENOM P19499; -.
Genome annotation databases
CMR P19499; MTH_1297.
Other
ProtoNet P19499.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Direct protein sequencing; Iron; Iron-sulfur; Metal-binding; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   281  280     Coenzyme F420 hydrogenase subunit beta. PRO_0000159229
CONFLICT   4     4        G -> C (in Ref. 2). 
CONFLICT   230   230        L -> F (in Ref. 1; AAA72190). 
Sequence information
Length: 281 AA [This is the length of the unprocessed precursor] Molecular weight: 30771 Da [This is the MW of the unprocessed precursor] CRC64: 350DCEE9029C09E1 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MVLGTYKEIV SARSTDREIQ KLAQDGGIVT GLLAYALDEG IIEGAVVAGP GKEFWKPEPM 

        70         80         90        100        110        120 
VAMTSDELKA AAGTKYTFSP NVLMLKKAVR QYGIEKLGTV AIPCQTMGIR KAQTYPFGVR 

       130        140        150        160        170        180 
FVADKIKLLV GIYCMENFPY TSLQTFICEK LGLNMELVEK MDIGKGKFWV YTQDDVYTLP 

       190        200        210        220        230        240 
LKETHGYEQA GCKICKDYVA ELADVSTGSV GSPDGWSTVI TRTDSGDSIL KQAVEAGIFE 

       250        260        270        280 
TKPIEEVKPG LGLLEKLSAQ KKEKAEKNIA ARKEMGLPTP Y 

P19499 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by kr flag YPRC Korea Mirror sites: Australia  Brazil  Canada  China  Switzerland
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!