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UniProtKB/Swiss-Prot entry P19496


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name FRHA_METTH
Primary accession number P19496
Secondary accession numbers None
Integrated into Swiss-Prot on February 1, 1991
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    September 2, 2008 (Entry version 60)
Name and origin of the protein
Protein name Coenzyme F420 hydrogenase subunit alpha
Synonyms EC 1.12.98.1
8-hydroxy-5-deazaflavin-reducing hydrogenase subunit alpha
FRH
Gene name
Name: frhA
OrderedLocusNames: MTH_1300
From
Methanobacterium thermoautotrophicum [TaxID: 187420] [HAMAP proteome]
Taxonomy Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
STRAIN=Delta H;
DOI=10.1021/bi00483a011; PubMed=2207102 [NCBI, ExPASy, EBI, Israel, Japan]
Alex L.A., Reeve J.N., Orme-Johnson W.H., Walsh C.T.;
"Cloning, sequence determination, and expression of the genes encoding the subunits of the nickel-containing 8-hydroxy-5-deazaflavin reducing hydrogenase from Methanobacterium thermoautotrophicum delta H.";
Biochemistry 29:7237-7244(1990).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Delta H;
PubMed=9371463 [NCBI, ExPASy, EBI, Israel, Japan]
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R., Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.;
"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics.";
J. Bacteriol. 179:7135-7155(1997).
[3]
PROTEIN SEQUENCE OF 2-31.
STRAIN=Delta H;
DOI=10.1021/bi00388a007; PubMed=3663585 [NCBI, ExPASy, EBI, Israel, Japan]
Fox J.A., Livingston D.J., Orme-Johnson W.H., Walsh C.T.;
"8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization.";
Biochemistry 26:4219-4227(1987).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
J02914; AAA72187.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE000666; AAB85780.1; ALT_INIT; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A35620; A35620.
RefSeq NP_276419.1; -.
3D structure databases
HSSP P13065; 1CC1. [HSSP ENTRY / PDB]
ModBase P19496.
Enzyme and pathway databases
BioCyc MTHE187420:MTH1300-MON; -.
Family and domain databases
InterPro IPR017682; Coenz_F420_hydrogenase_asu.
IPR001501; Ni-dep_hyd_lsu.
Graphical view of domain structure.
Pfam PF00374; NiFeSe_Hases; 1.
Pfam graphical view of domain structure.
PROSITE PS00507; NI_HGENASE_L_1; 1.
PS00508; NI_HGENASE_L_2; 1.
BLOCKS P19496.
Genome annotation databases
GeneID 1471017; -.
GenomeReviews AE000666_GR; MTH_1300.
KEGG mth:MTH1300; -.
NMPDR fig|187420.1.peg.1274; -.
Phylogenomic databases
HOGENOM P19496; -.
Genome annotation databases
CMR P19496; MTH_1300.
Other
ProtoNet P19496.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Direct protein sequencing; FAD; Metal-binding; Nickel; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   405  404     Coenzyme F420 hydrogenase subunit alpha. PRO_0000199723
METAL   63    63        Nickel (Potential). 
METAL   66    66        Nickel (Potential). 
METAL   380   380        Nickel (Potential). 
METAL   383   383        Nickel (Potential). 
Sequence information
Length: 405 AA [This is the length of the unprocessed precursor] Molecular weight: 44802 Da [This is the MW of the unprocessed precursor] CRC64: BB1C7C7F577CE1AB [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSERIVISPT SRQEGHAELV MEVDDEGIVT KGRYFSITPV RGLEKIVTGK APETAPVIVQ 

        70         80         90        100        110        120 
RICGVCPIPH TLASVEAIDD SLDIEVPKAG RLLRELTLAA HHVNSHAIHH FLIAPDFVPE 

       130        140        150        160        170        180 
NLMADAINSV SEIRKNAQYV VDMVAGEGIH PSDVRIGGMA DNITELARKR LYARLKQLKP 

       190        200        210        220        230        240 
KVDEHVELMI GLIEDKGLPK GLGVHNQPTL ASHQIYGDRT KFDLDRFTEV MPESWYDDPE 

       250        260        270        280        290        300 
IAKRACSTIP LYDGRNVEVG PRARMVEFQG FKERGVVAQH VARALEMKTA LARAIEILDE 

       310        320        330        340        350        360 
LDTSAPVRAD FDERGTGKLG VGAIEGPRGL DVHMAQVENG KIQFYSALVP TTWNIPTMGP 

       370        380        390        400 
ATEGFHHEYG PHVIRAYDPC LSCATHVMVV DDEDRSVIRD EMVRL 

P19496 in FASTA format

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