NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 49200 / DSM 3604 / VKM B-1801 / JJ;
DOI=10.1016/0378-1119(90)90473-5; PubMed=2227457 [NCBI, ExPASy, EBI, Israel, Japan]
Rousset M., Dermoun Z., Matchikian C.E., Belaich J.-P.;
"Cloning and sequencing of the locus encoding the large and small subunit genes of the periplasmic [NiFe]hydrogenase from Desulfovibrio fructosovorans.";
Gene 94:95-101(1990).

[2]

PROTEIN SEQUENCE OF 2-20.
PubMed=2154378 [NCBI, ExPASy, EBI, Israel, Japan]
Hatchikian C.E., Traore A.S., Fernandez V.M., Cammack R.;
"Characterization of the nickel-iron periplasmic hydrogenase from Desulfovibrio fructosovorans.";
Eur. J. Biochem. 187:635-643(1990).

Comments

CATALYTIC ACTIVITY: 2 H₂ + ferricytochrome c3 = 4 H⁺ + ferrocytochrome c3.
COFACTOR: Binds 1 nickel ion per subunit.
SUBUNIT: Heterodimer of a large and a small subunit.
SUBCELLULAR LOCATION: Periplasm.
MISCELLANEOUS: Perhaps the leader of the small subunit serves as a transport vehicle for both subunits.
SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M35333; AAA23372.2; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

JQ0762; S08199.

3D structure databases

PDB

1FRF; X-ray; 2.70 A; L=1-564.	[ExPASy / RCSB / EBI]
1YQW; X-ray; 1.83 A; Q/R/S=1-549.	[ExPASy / RCSB / EBI]
1YRQ; X-ray; 2.10 A; H/I/J/K/M/N=1-549.	[ExPASy / RCSB / EBI]

Detailed list of linked structures.

PDBsum

1FRF; -.
1YQW; -.
1YRQ; -.

ModBase

P18188.

Protein-protein interaction databases

DIP

DIP:6171N; -.

Family and domain databases

InterPro

IPR001501; Ni-dep_hyd_lsu.
Graphical view of domain structure.

Pfam

PF00374; NiFeSe_Hases; 1.
Pfam graphical view of domain structure.

PROSITE

PS00507; NI_HGENASE_L_1; 1.
PS00508; NI_HGENASE_L_2; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Direct protein sequencing; Metal-binding; Nickel; Oxidoreductase; Periplasm.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 549`	`548`	`Periplasmic [NiFe] hydrogenase large subunit.`	`PRO_0000013401`
`PROPEP`	`550 564`	`15`	`By similarity.`	`PRO_0000013402`
`METAL`	`72 72`		`Nickel (Potential).`
`METAL`	`75 75`		`Nickel (Potential).`
`METAL`	`543 543`		`Nickel (Potential).`
`METAL`	`546 546`		`Nickel (Potential).`
`STRAND`	`14 18`	`5`
`STRAND`	`23 26`	`4`
`STRAND`	`28 35`	`8`
`STRAND`	`38 46`	`9`
`HELIX`	`52 56`	`5`
`HELIX`	`61 63`	`3`
`HELIX`	`64 69`	`6`
`TURN`	`74 77`	`4`
`HELIX`	`78 91`	`14`
`HELIX`	`97 121`	`25`
`HELIX`	`124 126`	`3`
`HELIX`	`131 135`	`5`
`HELIX`	`138 146`	`9`
`HELIX`	`153 155`	`3`
`HELIX`	`157 172`	`16`
`HELIX`	`177 179`	`3`
`TURN`	`183 186`	`4`
`HELIX`	`195 223`	`29`
`STRAND`	`224 228`	`5`
`HELIX`	`240 243`	`4`
`HELIX`	`245 264`	`20`
`HELIX`	`266 276`	`11`
`HELIX`	`278 282`	`5`
`STRAND`	`289 291`	`3`
`STRAND`	`294 297`	`4`
`HELIX`	`302 308`	`7`
`STRAND`	`309 311`	`3`
`STRAND`	`314 316`	`3`
`HELIX`	`329 331`	`3`
`STRAND`	`332 335`	`4`
`HELIX`	`350 352`	`3`
`STRAND`	`369 371`	`3`
`STRAND`	`373 376`	`4`
`HELIX`	`385 395`	`11`
`HELIX`	`398 411`	`14`
`HELIX`	`415 418`	`4`
`HELIX`	`421 452`	`32`
`STRAND`	`464 474`	`11`
`STRAND`	`477 486`	`10`
`STRAND`	`489 496`	`8`
`HELIX`	`498 503`	`6`
`HELIX`	`514 519`	`6`
`HELIX`	`531 539`	`9`
`HELIX`	`544 548`	`5`

Sequence information

Length: 564 AA [This is the length of the unprocessed precursor]

Molecular weight: 61436 Da [This is the MW of the unprocessed precursor]

CRC64: AB3EBF47F364D849 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAESKPTPQS TFTGPIVVDP ITRIEGHLRI MVEVENGKVK DAWSSSQLFR GLEIILKGRD 

        70         80         90        100        110        120 
PRDAQHFTQR ACGVCTYVHA LASSRCVDDA VKVSIPANAR MMRNLVMASQ YLHDHLVHFY 

       130        140        150        160        170        180 
HLHALDWVDV TAALKADPNK AAKLAASIAP ARPGNSAKAL KAVQDKLKAF VESGQLGIFT 

       190        200        210        220        230        240 
NAYFLGGHKA YYLPPEVDLI ATAHYLEALH MQVKAASAMA ILGGKNPHTQ FTVVGGCSNY 

       250        260        270        280        290        300 
QGLTKDPLAN YLALSKEVCQ FVNECYIPDL LAVAGFYKDW GGIGGTSNYL AFGEFATDDS 

       310        320        330        340        350        360 
SPEKHLATSQ FPSGVITGRD LGKVDNVDLG AIYEDVKYSW YAPGGDGKHP YDGVTDPKYT 

       370        380        390        400        410        420 
KLDDKDHYSW MKAPRYKGKA MEVGPLARTF IAYAKGQPDF KKVVDMVLGK LSVPATALHS 

       430        440        450        460        470        480 
TLGRTAARGI ETAIVCANME KWIKEMADSG AKDNTLCAKW EMPEESKGVG LADAPRGALS 

       490        500        510        520        530        540 
HWIRIKGKKI DNFQLVVPST WNLGPRGAQG DKSPVEEALI GTPIADPKRP VEILRTVHAF 

       550        560 
DPCIACGVHV IEPETNEILK FKVC

P18188 in FASTA format

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View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools