NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 49200 / DSM 3604 / VKM B-1801 / JJ;
DOI=10.1016/0378-1119(90)90473-5; PubMed=2227457 [NCBI, ExPASy, EBI, Israel, Japan]
Rousset M., Dermoun Z., Matchikian C.E., Belaich J.-P.;
"Cloning and sequencing of the locus encoding the large and small subunit genes of the periplasmic [NiFe]hydrogenase from Desulfovibrio fructosovorans.";
Gene 94:95-101(1990).

[2]

PROTEIN SEQUENCE OF 50-60.
PubMed=2154378 [NCBI, ExPASy, EBI, Israel, Japan]
Hatchikian C.E., Traore A.S., Fernandez V.M., Cammack R.;
"Characterization of the nickel-iron periplasmic hydrogenase from Desulfovibrio fructosovorans.";
Eur. J. Biochem. 187:635-643(1990).

Comments

CATALYTIC ACTIVITY: 2 H₂ + ferricytochrome c3 = 4 H⁺ + ferrocytochrome c3.
COFACTOR: Binds 2 4Fe-4S clusters.
COFACTOR: Binds 1 3Fe-4S cluster.
SUBUNIT: Heterodimer of a large and a small subunit.
SUBCELLULAR LOCATION: Periplasm.
PTM: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M35333; AAA23371.2; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

JQ0761; S08198.

3D structure databases

PDB

1FRF; X-ray; 2.70 A; S=51-314.	[ExPASy / RCSB / EBI]
1YQW; X-ray; 1.83 A; A/B/C=51-314.	[ExPASy / RCSB / EBI]
1YRQ; X-ray; 2.10 A; A/B/C/D/F/G=51-314.	[ExPASy / RCSB / EBI]

Detailed list of linked structures.

PDBsum

1FRF; -.
1YQW; -.
1YRQ; -.

ModBase

P18187.

Protein-protein interaction databases

DIP

DIP:6172N; -.

Family and domain databases

InterPro

IPR001821; NiFe_hyd_ssu.
IPR013634; NiFe_hyd_ssu_N.
IPR006311; Tat.
Graphical view of domain structure.

Pfam

PF08425; NiFe_dehyd_N; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000310; NiFe_hyd_ssu; 1.

PRINTS

PR00614; NIHGNASESMLL.

TIGRFAMs

TIGR00391; hydA; 1.
TIGR01409; TAT_signal_seq; 1.

PROSITE

PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; 3Fe-4S; 4Fe-4S; Direct protein sequencing; Iron; Iron-sulfur; Metal-binding; Oxidoreductase; Periplasm; Signal.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 49`	`49`	`Tat-type signal.`
`CHAIN`	`50 314`	`265`	`Periplasmic [NiFe] hydrogenase small subunit.`	`PRO_0000013415`
`METAL`	`67 67`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`70 70`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`160 160`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`197 197`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`234 234`		`Iron-sulfur 2 (4Fe-4S); via pros nitrogen (By similarity).`
`METAL`	`237 237`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`262 262`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`268 268`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`277 277`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`295 295`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`298 298`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`STRAND`	`58 63`	`6`
`HELIX`	`69 74`	`6`
`TURN`	`78 81`	`4`
`HELIX`	`82 88`	`7`
`STRAND`	`91 95`	`5`
`TURN`	`96 98`	`3`
`HELIX`	`103 114`	`12`
`STRAND`	`120 129`	`10`
`HELIX`	`131 134`	`4`
`STRAND`	`137 139`	`3`
`HELIX`	`144 153`	`10`
`STRAND`	`158 162`	`5`
`HELIX`	`163 166`	`4`
`HELIX`	`170 172`	`3`
`HELIX`	`183 187`	`5`
`STRAND`	`192 194`	`3`
`STRAND`	`196 198`	`3`
`HELIX`	`201 213`	`13`
`HELIX`	`226 229`	`4`
`STRAND`	`230 232`	`3`
`HELIX`	`234 236`	`3`
`HELIX`	`240 244`	`5`
`HELIX`	`255 258`	`4`
`HELIX`	`264 266`	`3`
`HELIX`	`270 272`	`3`
`HELIX`	`277 280`	`4`
`TURN`	`283 285`	`3`
`TURN`	`288 292`	`5`
`HELIX`	`303 306`	`4`

Sequence information

Length: 314 AA [This is the length of the unprocessed precursor]

Molecular weight: 33621 Da [This is the MW of the unprocessed precursor]

CRC64: 48BE00FE51D23A68 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNFSVGLGRD DAEKRLVQNG VSRRDFMKFC ATVAAAMGMG PAFAPKVAEA LTAKHRPSVV 

        70         80         90        100        110        120 
WLHNAECTGC TEAAIRTIKP YIDALILDTI SLDYQETIMA AAGEAAEAAL HQALEGKDGY 

       130        140        150        160        170        180 
YLVVEGGLPT IDGGQWGMVA GHPMIETTKK AAAKAKGIIC IGTCSAYGGV QKAKPNPSQA 

       190        200        210        220        230        240 
KGVSEALGVK TINIPGCPPN PINFVGAVVH VLTKGIPDLD ENGRPKLFYG ELVHDNCPRL 

       250        260        270        280        290        300 
PHFEASEFAP SFDSEEAKKG FCLYELGCKG PVTYNNCPKV LFNQVNWPVQ AGHPCLGCSE 

       310 
PDFWDTMTPF YEQG

P18187 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools