[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=ATCC 1055 / DSM 2061; DOI=10.1007/BF00265053; PubMed=2250647 [NCBI, ExPASy, EBI, Israel, Japan] van Gorcom R.F.M., Boschloo J.G., Kuijvenhoven A., Lange J., van Vark A.J., Bos C.J., van Balken J.A.M., Pouwels P.H., van den Hondel C.A.M.J.J.; "Isolation and molecular characterisation of the benzoate-para-hydroxylase gene (bphA) of Aspergillus niger: a member of a new gene family of the cytochrome P450 superfamily."; Mol. Gen. Genet. 223:192-197(1990).
[2]	NUCLEOTIDE SEQUENCE [MRNA]. Higgins U.M., Darby R.M., Maddison A., Lamb D.C., Draper J.; "Yeast heterologous expression of a benzoate 4-hydroxylase (CYP53) from Aspergillus niger."; Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.
[3]	CHARACTERIZATION. STRAIN=T18.5; DOI=10.1006/abbi.2001.2534; PubMed=11594739 [NCBI, ExPASy, EBI, Israel, Japan] Faber B.W., van Gorcom R.F.M., Duine J.A.; "Purification and characterization of benzoate-para-hydroxylase, a cytochrome P450 (CYP53A1), from Aspergillus niger."; Arch. Biochem. Biophys. 394:245-254(2001).

Comments

FUNCTION: Responsible for cytochrome P450 dependent benzoate hydroxylation in microsomes; requires cprA as the mediator in electron donation from NADPH.
CATALYTIC ACTIVITY: Benzoate + NADPH + O₂ = 4-hydroxybenzoate + NADP⁺ + H₂O.
COFACTOR: Heme group (By similarity).
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X52521; CAA36753.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ347748; CAC69995.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S12015; S12015.

3D structure databases

HSSP

P14779; 1JPZ. [HSSP ENTRY / PDB]

ModBase

P17549.

Ontologies

GO:0005792;	Cellular component: microsome (inferred from direct assay from UniProtKB).
GO:0018664;	Molecular function: benzoate 4-monooxygenase activity (inferred from direct assay from UniProtKB).
GO:0055114;	Biological process: oxidation reduction (inferred from direct assay from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00463; EP450I.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Heme; Iron; Metal-binding; Monooxygenase; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 517`	`517`	`Benzoate 4-monooxygenase.`	`PRO_0000052038`
`METAL`	`461 461`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 517 AA [This is the length of the unprocessed precursor]

Molecular weight: 58016 Da [This is the MW of the unprocessed precursor]

CRC64: 63FFBFDF703EC30B [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLALLLSPYG AYLGLALLVL YYLLPYLKRA HLRDIPAPGL AAFTNFWLLL QTRRGHRFVV 

        70         80         90        100        110        120 
VDNAHKKYGK LVRIAPRHTS IADDGAIQAV YGHGNGFLKS DFYDAFVSIH RGLFNTRDRA 

       130        140        150        160        170        180 
EHTRKRKTVS HTFSMKSIGQ FEQYIHGNIE LFVKQWNRMA DTQRNPKTGF ASLDALNWFN 

       190        200        210        220        230        240 
YLAFDIIGDL AFGAPFGMLD KGKDFAEMRK TPDSPPSYVQ AVEVLNRRGE VSATLGCYPA 

       250        260        270        280        290        300 
LKPFAKYLPD SFFRDGIQAV EDLAGIAVAR VNERLRPEVM ANNTRVDLLA RLMEGKDSNG 

       310        320        330        340        350        360 
EKLGRAELTA EALTQLIAGS DTTSNTSCAI LYWCMRTPGV IEKLHKALDE AIPQDVDVPT 

       370        380        390        400        410        420 
HAMVKDIPYL QWVIWETMRI HSTSAMGLPR EIPAGNPPVT ISGHTFYPGD VVSVPSYTIH 

       430        440        450        460        470        480 
RSKEIWGPDA EQFVPERWDP ARLTPRQKAA FIPFSTGPRA CVGRNVAEME LLVICGTVFR 

       490        500        510 
LFEFEMQQEG PMETREGFLR KPLGLQVGMK RRQPGSA

P17549 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools