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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=JMP222;
DOI=10.1093/nar/18.11.3405; PubMed=2356133 [NCBI, ExPASy, EBI, Israel, Japan]
Kabisch M.,
Fortnagel P.;
"Nucleotide sequence of metapyrocatechase I (catechol 2,3-oxygenase I) gene mpcI from Alcaligenes eutrophus JMP222.";
Nucleic Acids Res. 18:3405-3405(1990).
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[2]
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FUNCTION IN CATABOLISM OF 3-HYDROXY DERIVATIVES OF PHENYLPROPIONIC ACID, COFACTOR, AND BIOPHYSICOCHEMICAL PROPERTIES.
PubMed=8752345 [NCBI, ExPASy, EBI, Israel, Japan]
Spence E.L.,
Kawamukai M.,
Sanvoisin J.,
Braven H.,
Bugg T.D.H.;
"Catechol dioxygenases from Escherichia coli (MhpB) and Alcaligenes eutrophus (MpcI): sequence analysis and biochemical properties of a third family of extradiol dioxygenases.";
J. Bacteriol. 178:5249-5256(1996).
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- FUNCTION: Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively. Also catalyzes the cleavage of catechol.
- CATALYTIC ACTIVITY: 3-(2,3-dihydroxyphenyl)propanoate + O2 = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate.
- CATALYTIC ACTIVITY: 2,3-dihydroxicinnamic acid + O2 = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate.
- COFACTOR: Fe(2+) ion.
- BIOPHYSICOCHEMICAL PROPERTIES:
| Kinetic parameters: |
KM=7.1 µM for 2,3-dihydroxyphenylpropionic acid (at 20 degrees Celsius and pH 8); | | KM=14 µM for 2,3-dihydroxycinnamic acid (at 20 degrees Celsius and pH 8); | | KM=59 µM for 3-ethylcatechol (at 20 degrees Celsius and pH 8); | | KM=130 µM for 3-methylcatechol (at 20 degrees Celsius and pH 8); | |
- PATHWAY: Aromatic compound metabolism; 3-phenylpropionic acid degradation.
- SUBUNIT: Homotetramer (By similarity).
- SIMILARITY: Belongs to the ligB/mhpB extradiol dioxygenase family.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 313 AA [This is the length of the unprocessed precursor] |
Molecular weight: 33143 Da [This is the MW of the unprocessed precursor] |
CRC64: E1506B3785E9D0F9 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MPIQLECLSH TPLHGYVDPA PEVVAEVERV QAAARDRVRA FDPELVVVFA PDHFNGFFYD
70 80 90 100 110 120
VMPPFCIGAA ATAIGDFKSL AGKLPVPADL ALSLAESVMA ADIDVALSHR MQVDHGCADA
130 140 150 160 170 180
LAALTGSLHR YPVIPVFINS VAPPMATLRR ARLLGDAVGR FLSRAGKRVL VVGSGGISHE
190 200 210 220 230 240
PPVPELAGAS EEVAERLIAG RNPSPESAAR QARTVAAAKS FVAGDSHLHP LNPEWDRAFL
250 260 270 280 290 300
SLLASGELTA VDGMTNDAIT RDGGKSAHEI RTWVAAFGAL AAYGPYRASL DFYRAIPEWI
310
AGFATMHAEP AAV
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P17295 in FASTA format |
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