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UniProtKB/Swiss-Prot entry P16027

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DHM1_METEX
Primary accession number P16027
Secondary accession numbers None
Integrated into Swiss-Prot on April 1, 1990
Sequence was last modified on April 1, 1990 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 75)
Name and origin of the protein
Protein name Methanol dehydrogenase subunit 1 [Precursor]
Synonyms EC 1.1.99.8
MDH large alpha subunit
MEDH
Gene name
Name: moxF
From
Methylobacterium extorquens (Protomonas extorquens) [TaxID: 408] 
Taxonomy Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Methylobacteriaceae; Methylobacterium.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=AM1 / NCIMB 9133;
DOI=10.1016/0378-1119(90)90457-3; PubMed=2116368 [NCBI, ExPASy, EBI, Israel, Japan]
Anderson D.J., Morris C.J., Nunn D.N., Anthony C., Lidstrom M.E.;
"Nucleotide sequence of the Methylobacterium extorquens AM1 moxF and moxJ genes involved in methanol oxidation.";
Gene 90:173-176(1990).
[2]
 PROTEIN SEQUENCE OF 28-53.
STRAIN=AM1 / NCIMB 9133;
PubMed=2504152 [NCBI, ExPASy, EBI, Israel, Japan]
Nunn D.N., Day D., Anthony C.;
"The second subunit of methanol dehydrogenase of Methylobacterium extorquens AM1.";
Biochem. J. 260:857-862(1989).
[3]
 REVIEW.
DOI=10.1089/15230860152664966; PubMed=11761326 [NCBI, ExPASy, EBI, Israel, Japan]
Anthony C.;
"Pyrroloquinoline quinone (PQQ) and quinoprotein enzymes.";
Antioxid. Redox Signal. 3:757-774(2001).
[4]
 DISULFIDE BONDS.
DOI=10.1038/nsb0294-102; PubMed=7656012 [NCBI, ExPASy, EBI, Israel, Japan]
Blake C.C.F., Ghosh M., Harlos K., Avezoux A., Anthony C.;
"The active site of methanol dehydrogenase contains a disulphide bridge between adjacent cysteine residues.";
Nat. Struct. Biol. 1:102-105(1994).
[5]
 X-RAY CRYSTALLOGRAPHY (1.94 ANGSTROMS).
DOI=10.1016/S0969-2126(01)00148-4; PubMed=7735834 [NCBI, ExPASy, EBI, Israel, Japan]
Ghosh M., Anthony C., Harlos K., Goodwin M.G., Blake C.;
"The refined structure of the quinoprotein methanol dehydrogenase from Methylobacterium extorquens at 1.94 A.";
Structure 3:177-187(1995).
[6]
 X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF MUTANT GLU-330, AND MUTAGENESIS OF CYS-130; CYS-131 AND ASP-330.
DOI=10.1021/bi002932l; PubMed=11502173 [NCBI, ExPASy, EBI, Israel, Japan]
Afolabi P.R., Mohammed F., Amaratunga K., Majekodunmi O., Dales S.L., Gill R., Thompson D., Cooper J.B., Wood S.P., Goodwin P.M., Anthony C.;
"Site-directed mutagenesis and X-ray crystallography of the PQQ-containing quinoprotein methanol dehydrogenase and its electron acceptor, cytochrome c(L).";
Biochemistry 40:9799-9809(2001).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M31108; AAA25380.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR JQ0706; JQ0706.
3D structure databases
PDB
1H4I; X-ray; 1.94 A; A/C=28-626.[ExPASy / RCSB / EBI]
1H4J; X-ray; 3.00 A; A/C/E/G=28-626.[ExPASy / RCSB / EBI]
1W6S; X-ray; 1.20 A; A/C=28-626.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1H4I; -.
1H4J; -.
1W6S; -.
ModBase P16027.
Enzyme and pathway databases
BioCyc MetaCyc:MON-3921; -.
Ontologies
GO
GO:0005886; Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0018468; Molecular function: alcohol dehydrogenase (acceptor) activity (inferred from electronic annotation from EC).
QuickGo view.
Family and domain databases
InterPro IPR017512; PQQ_MeOH/EtOH_DH.
IPR002372; PQQ_repeat.
IPR011047; Quino_AlcDHase-like.
IPR001479; Quinoprotein_DHase_PQQ_bac_CS.
Graphical view of domain structure.
Gene3D G3DSA:2.140.10.10; Quinoprotein_alc_DH-like; 1.
Pfam PF01011; PQQ; 5.
Pfam graphical view of domain structure.
SMART SM00564; PQQ; 3.
SMART graphical view of domain structure.
PROSITE PS00363; BACTERIAL_PQQ_1; 1.
PS00364; BACTERIAL_PQQ_2; 1.
BLOCKS P16027.
Other
ProtoNet P16027.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Calcium; Cell inner membrane; Cell membrane; Direct protein sequencing; Membrane; Metal-binding; Methanol utilization; Oxidoreductase; Periplasm; PQQ; Signal.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
SIGNAL   1    27  27      
CHAIN   28   626  599     Methanol dehydrogenase subunit 1. PRO_0000025566
ACT_SITE   330   330        Proton acceptor (By similarity). 
METAL   204   204        Calcium. 
METAL   288   288        Calcium. 
DISULFID   130   131         
DISULFID   413   442         
MUTAGEN   130   130        C->S: Inactive. 
MUTAGEN   131   131        C->S: Inactive. 
MUTAGEN   330   330        D->E: Lower affinity for methanol. 
HELIX   29    35  7      
TURN   61    63  3      
HELIX   64    66  3      
STRAND   67    74  8      
STRAND   86    88  3      
STRAND   91    95  5      
TURN   98   100  3      
STRAND   102   106  5      
STRAND   112   117  6      
HELIX   123   128  6      
STRAND   139   141  3      
STRAND   145   147  3      
STRAND   150   154  5      
STRAND   158   164  7      
TURN   165   167  3      
STRAND   170   175  6      
HELIX   179   181  3      
STRAND   190   192  3      
STRAND   195   198  4      
HELIX   203   205  3      
STRAND   210   215  6      
TURN   216   218  3      
STRAND   221   229  9      
HELIX   231   234  4      
TURN   238   243  6      
HELIX   245   247  3      
HELIX   252   255  4      
HELIX   261   264  4      
TURN   277   279  3      
STRAND   281   285  5      
HELIX   294   296  3      
STRAND   304   311  8      
TURN   312   314  3      
STRAND   317   324  8      
STRAND   338   343  6      
STRAND   349   356  8      
STRAND   360   366  7      
TURN   367   369  3      
STRAND   372   379  8      
STRAND   384   388  5      
TURN   390   392  3      
STRAND   395   397  3      
HELIX   399   401  3      
STRAND   409   414  6      
STRAND   426   428  3      
TURN   429   432  4      
STRAND   433   439  7      
STRAND   441   447  7      
STRAND   461   467  7      
TURN   473   476  4      
STRAND   480   485  6      
TURN   487   489  3      
STRAND   492   500  9      
STRAND   507   509  3      
TURN   510   512  3      
STRAND   513   517  5      
STRAND   521   527  7      
TURN   528   530  3      
STRAND   533   538  6      
STRAND   548   552  5      
STRAND   555   562  8      
TURN   566   569  4      
HELIX   570   574  5      
HELIX   582   584  3      
HELIX   585   588  4      
TURN   589   591  3      
HELIX   592   594  3      
STRAND   601   607  7      
TURN   616   619  4      
Sequence information
Length: 626 AA [This is the length of the unprocessed precursor] Molecular weight: 68434 Da [This is the MW of the unprocessed precursor] CRC64: 64988D0AFD2AD34C [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MSRFVTSVSA LAMLALAPAA LSSGAYANDK LVELSKSDDN WVMPGKNYDS NNFSDLKQIN 

        70         80         90        100        110        120 
KGNVKQLRPA WTFSTGLLNG HEGAPLVVDG KMYIHTSFPN NTFALGLDDP GTILWQDKPK 

       130        140        150        160        170        180 
QNPAARAVAC CDLVNRGLAY WPGDGKTPAL ILKTQLDGNV AALNAETGET VWKVENSDIK 

       190        200        210        220        230        240 
VGSTLTIAPY VVKDKVIIGS SGAELGVRGY LTAYDVKTGE QVWRAYATGP DKDLLLASDF 

       250        260        270        280        290        300 
NIKNPHYGQK GLGTGTWEGD AWKIGGGTNW GWYAYDPGTN LIYFGTGNPA PWNETMRPGD 

       310        320        330        340        350        360 
NKWTMTIFGR DADTGEAKFG YQKTPHDEWD YAGVNVMMLS EQKDKDGKAR KLLTHPDRNG 

       370        380        390        400        410        420 
IVYTLDRTDG ALVSANKLDD TVNVFKSVDL KTGQPVRDPE YGTRMDHLAK DICPSAMGYH 

       430        440        450        460        470        480 
NQGHDSYDPK RELFFMGINH ICMDWEPFML PYRAGQFFVG ATLNMYPGPK GDRQNYEGLG 

       490        500        510        520        530        540 
QIKAYNAITG DYKWEKMERF AVWGGTMATA GDLVFYGTLD GYLKARDSDT GDLLWKFKIP 

       550        560        570        580        590        600 
SGAIGYPMTY THKGTQYVAI YYGVGGWPGV GLVFDLADPT AGLGAVGAFK KLANYTQMGG 

       610        620 
GVVVFSLDGK GPYDDPNVGE WKSAAK
P16027 in FASTA format

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