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UniProtKB/Swiss-Prot entry P15047


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ENTA_ECOLI
Primary accession number P15047
Secondary accession numbers P77100 Q2MBK5
Integrated into Swiss-Prot on April 1, 1990
Sequence was last modified on April 1, 1990 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 77)
Name and origin of the protein
Protein name 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
Synonym EC 1.3.1.28
Gene name
Name: entA
OrderedLocusNames: b0596, JW0588
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-14.
PubMed=2521622 [NCBI, ExPASy, EBI, Israel, Japan]
Liu J., Duncan K., Walsh C.T.;
"Nucleotide sequence of a cluster of Escherichia coli enterobactin biosynthesis genes: identification of entA and purification of its product 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase.";
J. Bacteriol. 171:791-798(1989).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=2521621 [NCBI, ExPASy, EBI, Israel, Japan]
Nahlik M.S., Brickman T.J., Ozenberger B.A., McIntosh M.A.;
"Nucleotide sequence and transcriptional organization of the Escherichia coli enterobactin biosynthesis cistrons entB and entA.";
J. Bacteriol. 171:784-790(1989).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
"Sequence of minutes 4-25 of Escherichia coli.";
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M24148; AAA16103.1; -; Unassigned_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M24143; AAA76836.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U82598; AAB40796.1; ALT_INIT; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC73697.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAE76351.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A91904; DEECDB.
RefSeq AP_001243.1; -.
NP_415128.1; -.
3D structure databases
PDB
2FWM; X-ray; 2.00 A; X=1-248.[ExPASy / RCSB / EBI]
PDBsum 2FWM; -.
ModBase P15047.
Protein-protein interaction databases
DIP DIP:9511N; -.
IntAct P15047; -.
Enzyme and pathway databases
BioCyc EcoCyc:ENTA-MON; -.
Organism-specific databases
EchoBASE EB0255; -.
EcoGene EG10259; entA.
Family and domain databases
InterPro IPR002198; DHase_sc/Rdtase_SDR.
IPR003560; DHB_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR19410; ADH_short_C2; 1.
Pfam PF00106; adh_short; 1.
Pfam graphical view of domain structure.
PRINTS PR01397; DHBDHDRGNASE.
PR00080; SDRFAMILY.
PROSITE PS00061; ADH_SHORT; 1.
BLOCKS P15047.
Genome annotation databases
GeneID 945284; -.
GenomeReviews U00096_GR; b0596.
AP009048_GR; JW0588.
KEGG ecj:JW0588; -.
eco:b0596; -.
Phylogenomic databases
HOGENOM P15047; -.
Genome annotation databases
CMR P15047; b0596.
Other
ProtoNet P15047.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Complete proteome; Direct protein sequencing; Enterobactin biosynthesis; Ion transport; Iron; Iron transport; NAD; Oxidoreductase; Transport.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   248  248     2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase. PRO_0000054659
NP_BIND   9    33  25     NAD (By similarity). 
ACT_SITE   144   144        Proton acceptor (By similarity). 
BINDING   131   131        Substrate (By similarity). 
STRAND   7    12  6      
HELIX   16    27  12      
STRAND   31    37  7      
STRAND   45    50  6      
HELIX   56    69  14      
STRAND   75    78  4      
TURN   88    90  3      
HELIX   93   103  11      
HELIX   105   121  17      
STRAND   125   129  5      
HELIX   132   134  3      
HELIX   142   162  21      
HELIX   163   165  3      
STRAND   168   174  7      
HELIX   215   226  12      
HELIX   228   230  3      
STRAND   237   241  5      
TURN   242   247  6      
Sequence information
Length: 248 AA [This is the length of the unprocessed precursor] Molecular weight: 26250 Da [This is the MW of the unprocessed precursor] CRC64: E840488335AD317B [This is a checksum on the sequence]
        10         20         30         40         50         60 
MDFSGKNVWV TGAGKGIGYA TALAFVEAGA KVTGFDQAFT QEQYPFATEV MDVADAAQVA 

        70         80         90        100        110        120 
QVCQRLLAET ERLDALVNAA GILRMGATDQ LSKEDWQQTF AVNVGGAFNL FQQTMNQFRR 

       130        140        150        160        170        180 
QRGGAIVTVA SDAAHTPRIG MSAYGASKAA LKSLALSVGL ELAGSGVRCN VVSPGSTDTD 

       190        200        210        220        230        240 
MQRTLWVSDD AEEQRIRGFG EQFKLGIPLG KIARPQEIAN TILFLASDLA SHITLQDIVV 


DGGSTLGA 

P15047 in FASTA format

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