[1]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Kidney; DOI=10.1021/bi00456a004; PubMed=2340280 [NCBI, ExPASy, EBI, Israel, Japan] Johnson E.F., Walker D.L., Griffin K.J., Clark J.E., Okita R.T., Meurhoff A.S., Masters B.S.S.; "Cloning and expression of three rabbit kidney cDNAs encoding lauric acid omega-hydroxylases."; Biochemistry 29:873-879(1990).
[2]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Kidney; PubMed=2013275 [NCBI, ExPASy, EBI, Israel, Japan] Yokotani N., Kusunose E., Sogawa K., Kawashima H., Kinosaki M., Kusunose M., Fujii-Kuriyama Y.; "cDNA cloning and expression of the mRNA for cytochrome P-450kd which shows a fatty acid omega-hydroxylating activity."; Eur. J. Biochem. 196:531-536(1991).

Comments

FUNCTION: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
CATALYTIC ACTIVITY: Octane + reduced rubredoxin + O₂ = 1-octanol + oxidized rubredoxin + H₂O.
COFACTOR: Heme group (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.
INDUCTION: P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M28655; AAA31229.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X57209; CAA40493.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A34260; A34260.

3D structure databases

HSSP

P14779; 1JPZ. [HSSP ENTRY / PDB]

ModBase

P14579.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00463; EP450I.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

BLOCKS

P14579.

Genome annotation databases

Ensembl

ENSOCUG00000015041; Oryctolagus cuniculus. [Contig view]

Phylogenomic databases

HOVERGEN

P14579; -.

Other

ProtoNet

P14579.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome; Monooxygenase; Oxidoreductase; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`PROPEP`	`1 4`	`4`	`Probable.`	`PRO_0000003573`
`CHAIN`	`5 511`	`507`	`Cytochrome P450 4A5.`	`PRO_0000003574`
`TRANSMEM`	`11 31`	`21`	`Potential.`
`METAL`	`458 458`		`Iron (heme axial ligand) (By similarity).`
`BINDING`	`322 322`		`Heme (covalent; via 1 link) (By similarity).`
`CONFLICT`	`435 435`		`G -> S (in Ref. 2; CAA40493).`
`CONFLICT`	`477 477`		`V -> L (in Ref. 2; CAA40493).`

Sequence information

Length: 511 AA [This is the length of the unprocessed precursor]

Molecular weight: 58357 Da [This is the MW of the unprocessed precursor]

CRC64: 11D174BFC8BFA268 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSVSALSPTR LPGSLSGLLQ VAALLGLLLL LLKAAQLYLR RQWLLRALQQ FPCPPFHWLL 

        70         80         90        100        110        120 
GHSREFQMNQ ELQQILKWVE KFPRACPHWI GGNKVRVQLY DPDYMKVILG RSDPKSRGSY 

       130        140        150        160        170        180 
TFVAPWIGYG LLLLNGQPWF QHRRMLTPAF HYDILKPYVG LMVDSVQIML DKWEQLVSQD 

       190        200        210        220        230        240 
SSLEVFQDIS LMTLDTIMKC AFSYQGSVQL DSRNSQSYIQ AVGDLNNLVF ARVRNIFHQS 

       250        260        270        280        290        300 
DTIYRLSPEG RLSHRACQLA HEHTDRVIQQ RKAQLQQEGE LEKVRRKRRL DFLDVLLFAK 

       310        320        330        340        350        360 
MENGSSLSDQ DLRAEVDTFM FEGHDTTASG VSWIFYALAT HPEHQHRCRE EIQGLLGDGA 

       370        380        390        400        410        420 
SITWEHLDQM PYTTMCIKEA MRLYPPVPAI SRDLSSPVTF PDGRSLPKGF TVTLSIYGLH 

       430        440        450        460        470        480 
HNPNVWPNPE VFDPGRFTPG SARHSHAFLP FSGGARNCIG KQFAMNELKV AVALTLVRFE 

       490        500        510 
LLPDPTRIPK PTARLVLKSN NGIHLRLRKL Q

P14579 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools