[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-31. PubMed=3322743 [NCBI, ExPASy, EBI, Israel, Japan] Li C., Peck H.D. Jr., le Gall J., Przybyla A.E.; "Cloning, characterization, and sequencing of the genes encoding the large and small subunits of the periplasmic [NiFe]hydrogenase of Desulfovibrio gigas."; DNA 6:539-551(1987).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SEQUENCE REVISION. PubMed=2651421 [NCBI, ExPASy, EBI, Israel, Japan] Voordouw G., Menon N.K., le Gall J., Choi E.S., Peck H.D. Jr., Przybyla A.E.; "Analysis and comparison of nucleotide sequences encoding the genes for [NiFe] and [NiFeSe] hydrogenases from Desulfovibrio gigas and Desulfovibrio baculatus."; J. Bacteriol. 171:2894-2899(1989).
[3]	PROTEIN SEQUENCE OF 2-30. DOI=10.1016/0006-291X(87)90376-7; PubMed=3322275 [NCBI, ExPASy, EBI, Israel, Japan] Prickril B.C., He S.H., Li C., Menon N.K., Choi E.S., Przybyla A.E., Dervartanian D.V., Peck H.D. Jr., Fauque G., le Gall J., Teixeira M., Moura I., Moura J.J.G., Patil D., Huynh B.H.; "Identification of three classes of hydrogenase in the genus, Desulfovibrio."; Biochem. Biophys. Res. Commun. 149:369-377(1987).
[4]	X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS). DOI=10.1038/373580a0; PubMed=7854413 [NCBI, ExPASy, EBI, Israel, Japan] Volbeda A., Charon M.-H., Piras C., Hatchikian E.C., Frey M., Fontecilla-Camps J.-C.; "Crystal structure of the nickel-iron hydrogenase from Desulfovibrio gigas."; Nature 373:580-587(1995).

Comments

CATALYTIC ACTIVITY: 2 H₂ + ferricytochrome c3 = 4 H⁺ + ferrocytochrome c3.
COFACTOR: Binds 1 nickel ion per subunit.
SUBUNIT: Heterodimer of a large and a small subunit.
SUBCELLULAR LOCATION: Periplasm.
MISCELLANEOUS: Perhaps the leader of the small subunit serves as a transport vehicle for both subunits.
SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M18083; AAA23378.1; ALT_SEQ; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

B32315; HQDVLG.

3D structure databases

PDB

1FRV; X-ray; 2.85 A; B/D=1-536.	[ExPASy / RCSB / EBI]
1YQ9; X-ray; 2.35 A; H/I=1-536.	[ExPASy / RCSB / EBI]
2FRV; X-ray; 2.54 A; B/D/F/H/J/L=1-536.	[ExPASy / RCSB / EBI]

Detailed list of linked structures.

PDBsum

1FRV; -.
1YQ9; -.
2FRV; -.

ModBase

P12944.

Family and domain databases

InterPro

IPR001501; Ni-dep_hyd_lsu.
Graphical view of domain structure.

Pfam

PF00374; NiFeSe_Hases; 1.
Pfam graphical view of domain structure.

PROSITE

PS00507; NI_HGENASE_L_1; 1.
PS00508; NI_HGENASE_L_2; 1.

Other

P12944; -.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Direct protein sequencing; Metal-binding; Nickel; Oxidoreductase; Periplasm.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 536`	`535`	`Periplasmic [NiFe] hydrogenase large subunit.`	`PRO_0000013403`
`PROPEP`	`537 551`	`15`		`PRO_0000013404`
`METAL`	`65 65`		`Nickel.`
`METAL`	`68 68`		`Nickel.`
`METAL`	`530 530`		`Nickel.`
`METAL`	`533 533`		`Nickel.`
`STRAND`	`8 11`	`4`
`STRAND`	`16 19`	`4`
`STRAND`	`21 28`	`8`
`STRAND`	`31 39`	`9`
`HELIX`	`45 49`	`5`
`HELIX`	`54 56`	`3`
`HELIX`	`57 62`	`6`
`STRAND`	`66 68`	`3`
`HELIX`	`71 84`	`14`
`HELIX`	`90 114`	`25`
`HELIX`	`117 119`	`3`
`HELIX`	`124 128`	`5`
`HELIX`	`131 141`	`11`
`HELIX`	`148 163`	`16`
`HELIX`	`168 170`	`3`
`TURN`	`174 177`	`4`
`HELIX`	`186 214`	`29`
`STRAND`	`215 219`	`5`
`HELIX`	`231 234`	`4`
`HELIX`	`236 255`	`20`
`HELIX`	`257 267`	`11`
`HELIX`	`268 273`	`6`
`STRAND`	`280 282`	`3`
`HELIX`	`293 295`	`3`
`STRAND`	`296 298`	`3`
`STRAND`	`301 303`	`3`
`HELIX`	`316 318`	`3`
`STRAND`	`319 322`	`4`
`TURN`	`324 327`	`4`
`STRAND`	`328 331`	`4`
`HELIX`	`336 338`	`3`
`HELIX`	`350 352`	`3`
`STRAND`	`359 362`	`4`
`HELIX`	`371 380`	`10`
`HELIX`	`384 397`	`14`
`HELIX`	`401 404`	`4`
`HELIX`	`407 437`	`31`
`STRAND`	`451 461`	`11`
`STRAND`	`464 473`	`10`
`STRAND`	`476 483`	`8`
`HELIX`	`485 490`	`6`
`HELIX`	`501 506`	`6`
`HELIX`	`518 526`	`9`
`HELIX`	`531 535`	`5`

Sequence information

Length: 551 AA [This is the length of the unprocessed precursor]

Molecular weight: 61480 Da [This is the MW of the unprocessed precursor]

CRC64: 4CF99AD3F75189B0 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSEMQGNKIV VDPITRIEGH LRIEVEVEGG KIKNAWSMST LFRGLEMILK GRDPRDAQHF 

        70         80         90        100        110        120 
TQRACGVCTY VHALASVRAV DNCVGVKIPE NATLMRNLTM GAQYMHDHLV HFYHLHALDW 

       130        140        150        160        170        180 
VNVANALNAD PAKAARLAND LSPRKTTTES LKAVQAKVKA LVESGQLGIF TNAYFLGGHP 

       190        200        210        220        230        240 
AYVLPAEVDL IATAHYLEAL RVQVKAARAM AIFGAKNPHT QFTVVGGCTN YDSLRPERIA 

       250        260        270        280        290        300 
EFRKLYKEVR EFIEQVYITD LLAVAGFYKN WAGIGKTSNF LTCGEFPTDE YDLNSRYTPQ 

       310        320        330        340        350        360 
GVIWGNDLSK VDDFNPDLIE EHVKYSWYEG ADAHHPYKGV TKPKWTEFHG EDRYSWMKAP 

       370        380        390        400        410        420 
RYKGEAFEVG PLASVLVAYA KKHEPTVKAV DLVLKTLGVG PEALFSTLGR TAARGIQCLT 

       430        440        450        460        470        480 
AAQEVEVWLD KLEANVKAGK DDLYTDWQYP TESQGVGFVN APRGMLSHWI VQRGGKIENF 

       490        500        510        520        530        540 
QHVVPSTWNL GPRCAERKLS AVEQALIGTP IADPKRPVEI LRTVHSYDPC IACGVHVIDP 

       550 
ESNQVHKFRI L

P12944 in FASTA format

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View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools