NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=cv. Rosakrone;
TISSUE=Seedling;
PubMed=2247465 [NCBI, ExPASy, EBI, Israel, Japan]
Liaud M.-F., Zhang D.-X., Cerff R.;
"Differential intron loss and endosymbiotic transfer of chloroplast glyceraldehyde-3-phosphate dehydrogenase genes to the nucleus.";
Proc. Natl. Acad. Sci. U.S.A. 87:8918-8922(1990).

[2]

NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Rosakrone;
TISSUE=Seedling;
DOI=10.1007/BF00027337; PubMed=2562762 [NCBI, ExPASy, EBI, Israel, Japan]
Brinkmann H., Cerff R., Salomon M., Soll J.;
"Cloning and sequence analysis of cDNAs encoding the cytosolic precursors of subunits GapA and GapB of chloroplast glyceraldehyde-3-phosphate dehydrogenase from pea and spinach.";
Plant Mol. Biol. 13:81-94(1989).

Comments

CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NADP⁺ = 3-phospho-D-glyceroyl phosphate + NADPH.
PATHWAY: Carbohydrate biosynthesis; Calvin cycle .
SUBUNIT: Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1).
SUBCELLULAR LOCATION: Plastid, chloroplast.
MISCELLANEOUS: Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.
SIMILARITY: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X52148; CAA36396.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X15190; CAA33264.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S14243; DEPMNA.

3D structure databases

HSSP

P19866; 1NBO. [HSSP ENTRY / PDB]

SMR

P12858; 70-405.

ModBase

P12858.

Ontologies

GO:0047100;	Molecular function: glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR000173; GlycerAld_3-P_DHase.
IPR006424; Glyceraldehyde-3-P_DHase_1.
Graphical view of domain structure.

PANTHER

PTHR10836; GAP_DH; 1.

Pfam

PF02800; Gp_dh_C; 1.
PF00044; Gp_dh_N; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000149; GAP_DH; 1.

PRINTS

PR00078; G3PDHDRGNASE.

TIGRFAMs

TIGR01534; GAPDH-I; 1.

PS00071; GAPDH; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calvin cycle; Chloroplast; NADP; Oxidoreductase; Plastid; Transit peptide.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 68`	`68`	`Chloroplast.`
`CHAIN`	`69 405`	`337`	`Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic.`	`PRO_0000010423`
`NP_BIND`	`80 81`	`2`	`NADP (By similarity).`
`REGION`	`221 223`	`3`	`Glyceraldehyde 3-phosphate binding (By similarity).`
`REGION`	`280 281`	`2`	`Glyceraldehyde 3-phosphate binding (By similarity).`
`ACT_SITE`	`222 222`		`Nucleophile (By similarity).`
`BINDING`	`104 104`		`NADP (By similarity).`
`BINDING`	`149 149`		`NADP; via carbonyl oxygen (By similarity).`
`BINDING`	`252 252`		`Glyceraldehyde 3-phosphate (By similarity).`
`BINDING`	`267 267`		`Glyceraldehyde 3-phosphate (By similarity).`
`BINDING`	`303 303`		`Glyceraldehyde 3-phosphate (By similarity).`
`BINDING`	`385 385`		`NADP (By similarity).`
`SITE`	`249 249`	`1`	`Activates thiol group during catalysis (By similarity).`
`CONFLICT`	`194 194`		`G -> R (in Ref. 2; CAA33264).`

Sequence information

Length: 405 AA [This is the length of the unprocessed precursor]

Molecular weight: 43338 Da [This is the MW of the unprocessed precursor]

CRC64: 667FD75FD3EA6430 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MASATFSVAK PAIKANGKGF SEFSGLRNSS RHLPFSRKSS DDFHSLVTFQ TNAVGSSGGH 

        70         80         90        100        110        120 
KKSLVVEAKQ LKVAINGFGR IGRNFLRCWH GRKDSPLDVI AINDTGGVKQ ASHLLKYDST 

       130        140        150        160        170        180 
LGIFDADVKP VGTDGISVDG KVIKVVSDRN PANLPWKELG IDLVIEGTGV FVDREGAGRH 

       190        200        210        220        230        240 
ITAGAKKVLI TAPGKGDIPT YVVGVNADAY THADDIISNA SCTTNCLAPF VKVLDQKFGI 

       250        260        270        280        290        300 
IKGTMTTTHS YTGDQRLLDA SHRDLRRARA AALNIVPTST GAAKAVALVL PTLKGKLNGI 

       310        320        330        340        350        360 
ALRVPTPNVS VVDLVVQVSK KTFAEEVNEA FRESAAKELT GILSVCDEPL VSVDFRCTDV 

       370        380        390        400 
SSTVDSSLTM VMGDDLVKVI AWYDNEWGYS QRVVDLADIV ANNWK

P12858 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools