[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=3054886 [NCBI, ExPASy, EBI, Israel, Japan] Sayavedra-Soto L.A., Powell G.K., Evans H.J., Morris R.O.; "Nucleotide sequence of the genetic loci encoding subunits of Bradyrhizobium japonicum uptake hydrogenase."; Proc. Natl. Acad. Sci. U.S.A. 85:8395-8399(1988).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=USDA 110; DOI=10.1093/dnares/9.6.189; PubMed=12597275 [NCBI, ExPASy, EBI, Israel, Japan] Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.; "Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."; DNA Res. 9:189-197(2002).
[3]	NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 589-596. DOI=10.1016/0378-1119(94)90126-0; PubMed=8163174 [NCBI, ExPASy, EBI, Israel, Japan] Fu C., Maier R.J.; "Sequence and characterization of three genes within the hydrogenase gene cluster of Bradyrhizobium japonicum."; Gene 141:47-52(1994).

Comments

FUNCTION: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.
CATALYTIC ACTIVITY: H₂ + A = AH₂.
COFACTOR: Binds 1 nickel ion per subunit (By similarity).
SUBUNIT: Heterodimer of a large and a small subunit.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

J04114; AAA26219.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BA000040; BAC52206.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L24446; AAD13471.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

B31341; HQZJUL.

RefSeq

NP_773581.1; -.

3D structure databases

HSSP

P21852; 1H2R. [HSSP ENTRY / PDB]

ModBase

P12636.

Enzyme and pathway databases

BioCyc

BJAP224911:BLL6941-MON; -.

Ontologies

GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0033748;	Molecular function: hydrogenase (acceptor) activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001501; Ni-dep_hyd_lsu.
Graphical view of domain structure.

Pfam

PF00374; NiFeSe_Hases; 1.
Pfam graphical view of domain structure.

PROSITE

PS00507; NI_HGENASE_L_1; 1.
PS00508; NI_HGENASE_L_2; 1.

Genome annotation databases

GeneID

1048593; -.

GenomeReviews

BA000040_GR; bll6941.

KEGG

bja:bll6941; -.

NMPDR

fig|224911.1.peg.6941; -.

Phylogenomic databases

HOGENOM

P12636; -.

Genome annotation databases

CMR

P12636; bll6941.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Complete proteome; Membrane; Metal-binding; Nickel; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 596`	`596`	`Uptake hydrogenase large subunit.`	`PRO_0000199710`
`METAL`	`75 75`		`Nickel (Potential).`
`METAL`	`78 78`		`Nickel (Potential).`
`METAL`	`575 575`		`Nickel (Potential).`
`METAL`	`578 578`		`Nickel (Potential).`

Sequence information

Length: 596 AA [This is the length of the unprocessed precursor]

Molecular weight: 65923 Da [This is the MW of the unprocessed precursor]

CRC64: 0409EE8972F036B7 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MGIQTPNGFN LDNSGKRIVV DPVTRIEGHM RVEVNVDADN VIRNAVSTGT MWRGIEVILK 

        70         80         90        100        110        120 
NRDPRDAWAF TERICGVCTG THALTSVRAV ENALGITIPE NANSIRNLMQ LALQVHDHVV 

       130        140        150        160        170        180 
HFYHLHALDW VDVVSALSAD PRATSTLAQS ISNWPLSSPG YFKDLQTRLK KFVESGQLGP 

       190        200        210        220        230        240 
FKNGYWGSKA YKLPPEANLM AVAHYLEALD FQKEIVKIHT IFGGKNPHPN WLVGGVPCPI 

       250        260        270        280        290        300 
NVDGTGAVGA INMERLNLIS SIIDRLIEFN EMVYLPDVAA IGSFYKDWLY GGGLSGQSVL 

       310        320        330        340        350        360 
AYGDVPEHAN DYSAKSLKLP RGAIINGNLS EVFPVDHANP DEIQEFVVHS WYKYPDETKG 

       370        380        390        400        410        420 
LHPWDGVTEP NYVLGPNAKG TKTAIEQLDE GGKYSWIKAP RWKGHAMEVG PLARWVVGYA 

       430        440        450        460        470        480 
QNKSEFKDPV DKFLRDLNLP TSALFSTLGR TAARALESVW AGRQMRYFQD KLVANIKAGD 

       490        500        510        520        530        540 
SSTANVDKWK PESWPKEAKG VGFTEAPRGA LAHWIKIKDT KIDNYQCVVP TTWNGSPRDP 

       550        560        570        580        590 
KGNIGAFEAS LMNTPMVNPE QPLEILRTIH SFDPCLACST HVMSPDGQEL AKVKVR

P12636 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools