[1]	NUCLEOTIDE SEQUENCE [MRNA]. PubMed=3417684 [NCBI, ExPASy, EBI, Israel, Japan] Balcarek J.M., Theisen T.W., Cook M.N., Varrichio A., Hwang S.-M., Strohsacker M.W., Crooke S.T.; "Isolation and characterization of a cDNA clone encoding rat 5-lipoxygenase."; J. Biol. Chem. 263:13937-13941(1988).
[2]	SEQUENCE REVISION TO 667-670. DOI=10.1021/bi00076a003; PubMed=8518276 [NCBI, ExPASy, EBI, Israel, Japan] Minor W., Steczko J., Bolin J.T., Otwinowski Z., Axelrod B.; "Crystallographic determination of the active site iron and its ligands in soybean lipoxygenase L-1."; Biochemistry 32:6320-6323(1993).

Comments

CATALYTIC ACTIVITY: Arachidonate + O₂ = leukotriene A₄ + H₂O.
COFACTOR: Binds 1 iron ion per subunit (By similarity).
COFACTOR: Binds 2 calcium ions per subunit (By similarity).
PATHWAY: Lipid metabolism; leukotriene A4 biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein (By similarity). Note=Calcium binding promotes binding to membranes (By similarity).
SIMILARITY: Belongs to the lipoxygenase family.
SIMILARITY: Contains 1 lipoxygenase domain.
SIMILARITY: Contains 1 PLAT domain.
SEQUENCE CAUTION:
- Sequence=AAA41538.1; Type=Frameshift; Positions=667;

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

J03960; AAA41538.1; ALT_FRAME; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

A30882; A30882.

NP_036954.1; -.

3D structure databases

HSSP

P12530; 1LOX. [HSSP ENTRY / PDB]

ModBase

P12527.

Organism-specific databases

RGD

2096; Alox5.

Gene expression databases

ArrayExpress

P12527; -.

GermOnline

ENSRNOG00000012972; Rattus norvegicus.

Family and domain databases

InterPro

IPR000907; LipOase.
IPR013819; LipOase_C.
IPR001024; LipOase_LH2.
IPR001885; LipOase_mml.
Graphical view of domain structure.

Gene3D

G3DSA:2.60.60.20; Lipase_LipOase; 1.

PANTHER

PTHR11771; LipOase; 1.

Pfam

PF00305; Lipoxygenase; 1.
PF01477; PLAT; 1.
Pfam graphical view of domain structure.

PRINTS

PR00087; LIPOXYGENASE.
PR00467; MAMLPOXGNASE.

SMART

SM00308; LH2; 1.
SMART graphical view of domain structure.

PROSITE

PS00711; LIPOXYGENASE_1; 1.
PS00081; LIPOXYGENASE_2; 1.
PS51393; LIPOXYGENASE_3; 1.
PS50095; PLAT; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

P12527.

Genome annotation databases

Ensembl

ENSRNOG00000012972; Rattus norvegicus. [Contig view]

GeneID

25290; -.

KEGG

rno:25290; -.

Phylogenomic databases

HOVERGEN

P12527; -.

Other

ProtoNet

P12527.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calcium; Cytoplasm; Dioxygenase; Iron; Leukotriene biosynthesis; Membrane; Metal-binding; Oxidoreductase; Phosphoprotein.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 673`	`672`	`Arachidonate 5-lipoxygenase.`	`PRO_0000220696`
`DOMAIN`	`2 117`	`116`	`PLAT.`
`DOMAIN`	`118 673`	`556`	`Lipoxygenase.`
`METAL`	`17 17`		`Calcium 1; via carbonyl oxygen; structural (By similarity).`
`METAL`	`18 18`		`Calcium 2; via carbonyl oxygen; structural (By similarity).`
`METAL`	`19 19`		`Calcium 2; structural (By similarity).`
`METAL`	`44 44`		`Calcium 2; structural (By similarity).`
`METAL`	`45 45`		`Calcium 2; via carbonyl oxygen; structural (By similarity).`
`METAL`	`47 47`		`Calcium 2; structural (By similarity).`
`METAL`	`79 79`		`Calcium 1; via carbonyl oxygen; structural (By similarity).`
`METAL`	`80 80`		`Calcium 1; via carbonyl oxygen; structural (By similarity).`
`METAL`	`367 367`		`Iron; catalytic (By similarity).`
`METAL`	`372 372`		`Iron; catalytic (By similarity).`
`METAL`	`550 550`		`Iron; catalytic (By similarity).`
`METAL`	`554 554`		`Iron; catalytic (By similarity).`
`METAL`	`673 673`		`Iron; via carboxylate; catalytic (By similarity).`
`MOD_RES`	`271 271`		`Phosphoserine (By similarity).`
`MOD_RES`	`523 523`		`Phosphoserine (By similarity).`

Sequence information

Length: 673 AA [This is the length of the unprocessed precursor]

Molecular weight: 78087 Da [This is the MW of the unprocessed precursor]

CRC64: 536B0BC27CB0A608 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MPSYTVTVAT GSQWFAGTDD YIYLSLIGSA GCSEKHLLDK AFYNDFERGG RDSYDVTVDE 

        70         80         90        100        110        120 
ELGEIYLVKI EKRKYRLHDD WYLKYITLKT PHDYIEFPCY RWITGEGEIV LRDGCAKLAR 

       130        140        150        160        170        180 
DDQIHILKQH RRKELETRQK QYRWMEWNPG FPLSIDAKCH KDLPRDIQFD SEKGVDFVLN 

       190        200        210        220        230        240 
YSKAMENLFI NRFMHMFQSS WHDFADFEKI FVKISNTISE RVKNHWQEDL MFGYQFLNGC 

       250        260        270        280        290        300 
NPVLIKRCTE LPKKLPVTTE MVECSLERQL SLEQEVQEGN IFIVDYELLD GIDANKTDPC 

       310        320        330        340        350        360 
THQFLAAPIC LLYKNLANKI VPIAIQLNQT PGEKNPIFLP TDSKYDWLLA KIWVRSSDFH 

       370        380        390        400        410        420 
IHQTITHLLR THLVSEVFGI AMYRQLPAVH PLFKLLVAHV RFTIAINTKA REQLNCEYGL 

       430        440        450        460        470        480 
FDKANATGGG GHVQMVQRAV QDLTYSSLCF PEAIKARGMD NTEDIPYYFY RDDGLLVWEA 

       490        500        510        520        530        540 
IQSFTTEVVS IYYEDDQVVE EDQELQDFVK DVYVYGMRGR KASGFPKSIK SREKLSEYLT 

       550        560        570        580        590        600 
VVIFTASAQH AAVNFGQYDW CSWIPNAPPT MRAPPPTAKG VVTIEQIVDT LPDRGRSCWH 

       610        620        630        640        650        660 
LGAVWALSQF QENELFLGMY PEEHFIEKPV KEAMIRFRKN LEAIVSVIAE RNKNKKLPYY 

       670 
YLSPDRIPNS VAI

P12527 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools