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UniProtKB/Swiss-Prot entry P10614


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CP51_YEAST
Primary accession number P10614
Secondary accession numbers None
Integrated into Swiss-Prot on July 1, 1989
Sequence was last modified on July 1, 1989 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 81)
Name and origin of the protein
Protein name Cytochrome P450 51
Synonyms EC 1.14.13.70
CYPLI
P450-LIA1
Sterol 14-alpha demethylase
Lanosterol 14-alpha demethylase
P450-14DM
Gene name
Name: ERG11
Synonyms: CYP51
OrderedLocusNames: YHR007C
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=3322742 [NCBI, ExPASy, EBI, Israel, Japan]
Kalb V.F., Woods C.W., Turi T.G., Dey C.R., Sutter T.R., Loper J.C.;
"Primary structure of the P450 lanosterol demethylase gene from Saccharomyces cerevisiae.";
DNA 6:529-537(1987).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/S0006-291X(88)81087-8; PubMed=3046615 [NCBI, ExPASy, EBI, Israel, Japan]
Ishida N., Aoyama Y., Hatanaka R., Oyama Y., Imajo S., Ishiguro M., Oshima T., Nakazato H., Noguchi T., Maitra U.S., Mohan V.P., Sprinson D.B., Yoshida Y.;
"A single amino acid substitution converts cytochrome P450(14DM) to an inactive form, cytochrome P450SG1: complete primary structures deduced from cloned DNAS.";
Biochem. Biophys. Res. Commun. 155:317-323(1988).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=8091229 [NCBI, ExPASy, EBI, Israel, Japan]
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.;
"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.";
Science 265:2077-2082(1994).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 444-530.
DOI=10.1016/0378-1119(86)90021-1; PubMed=3542713 [NCBI, ExPASy, EBI, Israel, Japan]
Kalb V.F., Loper J.C., Dey C.R., Woods C.W., Sutter T.R.;
"Isolation of a cytochrome P-450 structural gene from Saccharomyces cerevisiae.";
Gene 45:237-245(1986).
[5]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[6]
INTERACTION WITH ERG28.
DOI=10.1194/jlr.M500153-JLR200; PubMed=15995173 [NCBI, ExPASy, EBI, Israel, Japan]
Mo C., Bard M.;
"Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex.";
J. Lipid Res. 46:1991-1998(2005).
[7]
TOPOLOGY [LARGE SCALE ANALYSIS].
DOI=10.1073/pnas.0604075103; PubMed=16847258 [NCBI, ExPASy, EBI, Israel, Japan]
Kim H., Melen K., Oesterberg M., von Heijne G.;
"A global topology map of the Saccharomyces cerevisiae membrane proteome.";
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
[8]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458, AND MASS SPECTROMETRY.
DOI=10.1021/pr060559j; PubMed=17330950 [NCBI, ExPASy, EBI, Israel, Japan]
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.;
"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.";
J. Proteome Res. 6:1190-1197(2007).
[9]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 0:0-0(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M18109; AAA34379.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M15663; AAA34837.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M21483; AAA34546.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M21484; AAA34547.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U10555; AAB68433.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A27491; A27491.
RefSeq NP_011871.1; -.
3D structure databases
ModBase P10614.
Protein-protein interaction databases
DIP DIP:7886N; -.
Organism-specific databases
CYGD YHR007c; -.
SGD S000001049; ERG11.
Yeast-GFP YHR007C.
Gene expression databases
ArrayExpress P10614; -.
GermOnline YHR007C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005783; Cellular component: endoplasmic reticulum (inferred from direct assay from SGD).
QuickGo view.
Family and domain databases
InterPro IPR001128; Cyt_P450.
IPR002403; Cyt_P450_E_grp-IV.
Graphical view of domain structure.
Gene3D G3DSA:1.10.630.10; Cyt_P450; 1.
PANTHER PTHR19383; Cyt_P450; 1.
Pfam PF00067; p450; 1.
Pfam graphical view of domain structure.
PRINTS PR00465; EP450IV.
PR00385; P450.
PROSITE PS00086; CYTOCHROME_P450; 1.
BLOCKS P10614.
Proteomic databases
PeptideAtlas P10614; -.
Genome annotation databases
Ensembl YHR007C; Saccharomyces cerevisiae. [Contig view]
GeneID 856398; -.
GenomeReviews U00093_GR; YHR007C.
KEGG sce:YHR007C; -.
NMPDR fig|4932.3.peg.3015; -.
Phylogenomic databases
HOGENOM P10614; -.
Other
DrugBank DB00257; Clotrimazole.
DB01127; Econazole.
DB00196; Fluconazole.
DB01026; Ketoconazole.
DB01263; Posaconazole.
DB01153; Sertaconazole.
DB00251; Terconazole.
DB01007; Tioconazole.
DB00582; Voriconazole.
LinkHub P10614; -.
ProtoNet P10614.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Heme; Iron; Lipid synthesis; Membrane; Metal-binding; Monooxygenase; NADP; Oxidoreductase; Phosphoprotein; Steroid biosynthesis; Sterol biosynthesis; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   530  530     Cytochrome P450 51. PRO_0000052012
TOPO_DOM   1    20  20     Extracellular (Potential). 
TRANSMEM   21    41  21     Potential. 
TOPO_DOM   42   530  489     Cytoplasmic (Potential). 
METAL   470   470        Iron (heme axial ligand) (By similarity). 
MOD_RES   458   458        Phosphoserine. 
CONFLICT   433   433        K -> N (in Ref. 2; AAA34546/AAA34547). 
Sequence information
Length: 530 AA [This is the length of the unprocessed precursor] Molecular weight: 60720 Da [This is the MW of the unprocessed precursor] CRC64: 646960BBA0E17979 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSATKSIVGE ALEYVNIGLS HFLALPLAQR ISLIIIIPFI YNIVWQLLYS LRKDRPPLVF 

        70         80         90        100        110        120 
YWIPWVGSAV VYGMKPYEFF EECQKKYGDI FSFVLLGRVM TVYLGPKGHE FVFNAKLADV 

       130        140        150        160        170        180 
SAEAAYAHLT TPVFGKGVIY DCPNSRLMEQ KKFVKGALTK EAFKSYVPLI AEEVYKYFRD 

       190        200        210        220        230        240 
SKNFRLNERT TGTIDVMVTQ PEMTIFTASR SLLGKEMRAK LDTDFAYLYS DLDKGFTPIN 

       250        260        270        280        290        300 
FVFPNLPLEH YRKRDHAQKA ISGTYMSLIK ERRKNNDIQD RDLIDSLMKN STYKDGVKMT 

       310        320        330        340        350        360 
DQEIANLLIG VLMGGQHTSA ATSAWILLHL AERPDVQQEL YEEQMRVLDG GKKELTYDLL 

       370        380        390        400        410        420 
QEMPLLNQTI KETLRMHHPL HSLFRKVMKD MHVPNTSYVI PAGYHVLVSP GYTHLRDEYF 

       430        440        450        460        470        480 
PNAHQFNIHR WNKDSASSYS VGEEVDYGFG AISKGVSSPY LPFGGGRHRC IGEHFAYCQL 

       490        500        510        520        530 
GVLMSIFIRT LKWHYPEGKT VPPPDFTSMV TLPTGPAKII WEKRNPEQKI 

P10614 in FASTA format

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View entry in raw text format (no links)
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