NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=CE2010;
PubMed=11160798 [NCBI, ExPASy, EBI, Israel, Japan]
Ohta Y., Maeda M., Kudo T.;
"Pseudomonas putida CE2010 can degrade biphenyl by a mosaic pathway encoded by the tod operon and cmtE, which are identical to those of P. putida F1 except for a single base difference in the operator-promoter region of the cmt operon.";
Microbiology 147:31-41(2001).

Comments

CATALYTIC ACTIVITY: Cis-5,6-dihydroxy-4-isopropylcyclohexa-1,3-dienecarboxylate + NAD⁺ = 2,3-dihydroxy-p-cumate + NADH.
PATHWAY: Aromatic compound metabolism; p-cumic acid degradation; acetaldehyde and pyruvate from p-cumic acid: step 2/7 .
SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB042508; BAB17774.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P50163; 2AE1. [HSSP ENTRY / PDB]

ModBase

P0C622.

Ontologies

GO:0018511;	Molecular function: 2,3-dihydroxy-2,3-dihydro-p-cumate dehydrogenase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR002198; DHase_sc/Rdtase_SDR.
IPR002347; Glc/ribitol_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR19410; ADH_short_C2; 1.

Pfam

PF00106; adh_short; 1.
Pfam graphical view of domain structure.

PRINTS

PR00081; GDHRDH.
PR00080; SDRFAMILY.

PROSITE

PS00061; ADH_SHORT; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Aromatic hydrocarbons catabolism; NAD; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 259`	`259`	`2,3-dihydroxy-2,3-dihydro-p-cumate dehydrogenase.`	`PRO_0000054550`
`NP_BIND`	`18 42`	`25`	`NAD (By similarity).`
`ACT_SITE`	`163 163`		`Proton acceptor (By similarity).`

Sequence information

Length: 259 AA [This is the length of the unprocessed precursor]

Molecular weight: 27310 Da [This is the MW of the unprocessed precursor]

CRC64: B4CD9C3DDC833A8B [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSTLSRNALP LEGQVAVVTG GAHGIGLGIV ERLLGLGARV TASDIDESGL SLLCERLAAK 

        70         80         90        100        110        120 
HADAIAVHAA DLSEEQGAQG LHRAAVERFG SVQILVNCAG GGVIRPFLEH TPETLKATID 

       130        140        150        160        170        180 
RNLWTALWCS RVFLPDMLAR QYGRIINIGA DSVRNGLPDH AAYNAAKGGM HGLTTGLARE 

       190        200        210        220        230        240 
FARQGVTVNT VAPCAVNTEV WVRIKNANPE LAQRFLDVIP MGRVGEIEEV ASMVGYLAQP 

       250 
EAAFVTGQVI SVNGGSTML

P0C622 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools