NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=8226631 [NCBI, ExPASy, EBI, Israel, Japan]
Denome S.A., Stanley D.C., Olson E.S., Young K.D.;
"Metabolism of dibenzothiophene and naphthalene in Pseudomonas strains: complete DNA sequence of an upper naphthalene catabolic pathway.";
J. Bacteriol. 175:6890-6901(1993).

Comments

FUNCTION: Component of the naphthalene dioxygenase (NDO) multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into naphthalene to form cis-naphthalene dihydrodiol. The beta subunit may be responsible for the substrate specificity of the enzyme.
CATALYTIC ACTIVITY: Naphthalene + NADH + O₂ = (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD⁺.
COFACTOR: Binds 1 2Fe-2S cluster (By similarity).
COFACTOR: Binds 1 iron ion (By similarity).
PATHWAY: Aromatic compound metabolism; naphtalene degradation .
SUBUNIT: Naphthalene dioxygenase (NDO) multicomponent enzyme system is composed of an electron transfer component and an iron sulfur protein (ISP). The electron transfer component is composed of ferredoxin reductase and ferredoxin (doxA), and ISP is composed of a large alpha subunit (doxB) and a small beta subunit (doxD) (Probable).
SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family.

Copyright

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Cross-references

Sequence databases

EMBL

M60405; AAA16127.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S27634; S27634.

3D structure databases

PDB

2HMJ; X-ray; 1.50 A; B=1-194.	[ExPASy / RCSB / EBI]
2HMK; X-ray; 1.65 A; B=1-194.	[ExPASy / RCSB / EBI]
2HML; X-ray; 1.80 A; B=1-194.	[ExPASy / RCSB / EBI]
2HMM; X-ray; 1.60 A; B=1-194.	[ExPASy / RCSB / EBI]
2HMN; X-ray; 1.70 A; B=1-194.	[ExPASy / RCSB / EBI]
2HMO; X-ray; 1.60 A; B=1-194.	[ExPASy / RCSB / EBI]

Detailed list of linked structures.

PDBsum

2HMJ; -.
2HMK; -.
2HML; -.
2HMM; -.
2HMN; -.
2HMO; -.

ModBase

P0A113.

Family and domain databases

InterPro

IPR000391; Rng_hydr_dOase-B.
Graphical view of domain structure.

Pfam

PF00866; Ring_hydroxyl_B; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Aromatic hydrocarbons catabolism; Dioxygenase; NAD; Oxidoreductase; Plasmid.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 194`	`194`	`Naphthalene 1,2-dioxygenase subunit beta.`	`PRO_0000085073`
`TURN`	`5 7`	`3`
`HELIX`	`13 24`	`12`
`HELIX`	`28 46`	`19`
`HELIX`	`50 57`	`8`
`STRAND`	`58 69`	`12`
`STRAND`	`84 90`	`7`
`HELIX`	`92 103`	`12`
`HELIX`	`108 110`	`3`
`STRAND`	`115 127`	`13`
`STRAND`	`134 147`	`14`
`TURN`	`148 150`	`3`
`STRAND`	`151 165`	`15`
`HELIX`	`167 169`	`3`
`STRAND`	`171 179`	`9`
`STRAND`	`183 185`	`3`

Sequence information

Length: 194 AA [This is the length of the unprocessed precursor]

Molecular weight: 22935 Da [This is the MW of the unprocessed precursor]

CRC64: 168D2E1535E66416 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MMINIQEDKL VSAHDAEEIL RFFNCHDSAL QQEATTLLTQ EAHLLDIQAY RAWLEHCVGS 

        70         80         90        100        110        120 
EVQYQVISRE LRAASERRYK LNEAMNVYNE NFQQLKVRVE HQLDPQNWGN SPKLRFTRFI 

       130        140        150        160        170        180 
TNVQAAMDVN DKELLHIRSN VILHRARRGN QVDVFYAARE DKWKRGEGGV RKLVQRFVDY 

       190 
PERILQTHNL MVFL

P0A113 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools