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UniProtKB/Swiss-Prot entry P08516


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CP4AA_RAT
Primary accession number P08516
Secondary accession number Q5EBD8
Integrated into Swiss-Prot on August 1, 1988
Sequence was last modified on May 1, 2007 (Sequence version 2)
Annotations were last modified on    September 2, 2008 (Entry version 85)
Name and origin of the protein
Protein name Cytochrome P450 4A10
Synonyms EC 1.14.15.3
CYPIVA10
Lauric acid omega-hydroxylase
P450-LA-omega 1
P452
Gene name
Name: Cyp4a10
Synonyms: Cyp4a-1, Cyp4a1
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Liver;
PubMed=3027069 [NCBI, ExPASy, EBI, Israel, Japan]
Hardwick J.P., Song B.-J., Huberman E., Gonzalez F.J.;
"Isolation, complementary DNA sequence, and regulation of rat hepatic lauric acid omega-hydroxylase (cytochrome P-450LA omega). Identification of a new cytochrome P-450 gene family.";
J. Biol. Chem. 262:801-810(1987).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=Wistar;
TISSUE=Liver;
DOI=10.1016/0014-5793(88)80055-3; PubMed=3410047 [NCBI, ExPASy, EBI, Israel, Japan]
Earnshaw D., Dale J.W., Goldfarb P.S., Gibson G.G.;
"Differential splicing in the 3' non-coding region of rat cytochrome P-452 (P450 IVA1) mRNA.";
FEBS Lett. 236:357-361(1988).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
TISSUE=Liver;
PubMed=2766932 [NCBI, ExPASy, EBI, Israel, Japan]
Kimura S., Hanioka N., Matsunaga E., Gonzalez F.J.;
"The rat clofibrate-inducible CYP4A gene subfamily. I. Complete intron and exon sequence of the CYP4A1 and CYP4A2 genes, unique exon organization, and identification of a conserved 19-bp upstream element.";
DNA 8:503-516(1989).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Liver;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[5]
PROTEIN SEQUENCE OF 1-22.
DOI=10.1016/0003-9861(92)90714-8; PubMed=1567203 [NCBI, ExPASy, EBI, Israel, Japan]
Okita R.T., Okita J.R.;
"Characterization of a cytochrome P450 from di(2-ethylhexyl) phthalate-treated rats which hydroxylates fatty acids.";
Arch. Biochem. Biophys. 294:475-481(1992).
[6]
COVALENT HEME ATTACHMENT.
DOI=10.1074/jbc.M009969200; PubMed=11139583 [NCBI, ExPASy, EBI, Israel, Japan]
Hoch U., Ortiz de Montellano P.R.;
"Covalently linked heme in cytochrome P4504A fatty acid hydroxylases.";
J. Biol. Chem. 276:11339-11346(2001).
[7]
COVALENT HEME ATTACHMENT, AND MUTAGENESIS OF GLU-320.
DOI=10.1074/jbc.M112155200; PubMed=11821421 [NCBI, ExPASy, EBI, Israel, Japan]
LeBrun L.A., Hoch U., Ortiz de Montellano P.R.;
"Autocatalytic mechanism and consequences of covalent heme attachment in the cytochrome P4504A family.";
J. Biol. Chem. 277:12755-12761(2002).
Comments
  • FUNCTION: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
  • CATALYTIC ACTIVITY: Octane + reduced rubredoxin + O2 = 1-octanol + oxidized rubredoxin + H2O.
  • COFACTOR: Heme group (By similarity).
  • SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
  • INDUCTION: By clofibrate.
  • SIMILARITY: Belongs to the cytochrome P450 family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M14972; AAA41061.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X07259; CAA30245.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M57718; AAA41038.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC089761; AAH89761.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S01336; O4RTLO.
RefSeq NP_695219.1; -.
NP_787031.1; -.
UniGene Rn.92401
3D structure databases
HSSP P14779; 1JPZ. [HSSP ENTRY / PDB]
ModBase P08516.
Organism-specific databases
RGD 68945; Cyp4a1.
Gene expression databases
ArrayExpress P08516; -.
GermOnline ENSRNOG00000009597; Rattus norvegicus.
Ontologies
GO
GO:0005789; Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.
Family and domain databases
InterPro IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.
Gene3D G3DSA:1.10.630.10; Cyt_P450; 1.
PANTHER PTHR19383; Cyt_P450; 1.
Pfam PF00067; p450; 1.
Pfam graphical view of domain structure.
PRINTS PR00463; EP450I.
PR00385; P450.
PROSITE PS00086; CYTOCHROME_P450; 1.
BLOCKS P08516.
Genome annotation databases
Ensembl ENSRNOG00000009597; Rattus norvegicus. [Contig view]
GeneID 170544; -.
50549; -.
KEGG rno:170544; -.
rno:50549; -.
Phylogenomic databases
HOVERGEN P08516; -.
Other
ProtoNet P08516.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Direct protein sequencing; Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome; Monooxygenase; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   509  509     Cytochrome P450 4A10. PRO_0000051816
METAL   456   456        Iron (heme axial ligand). 
BINDING   320   320        Heme (covalent; via 1 link). 
MUTAGEN   320   320        E->A: Loss of heme binding. 
CONFLICT   341   341        E -> K (in Ref. 1; AAA41061 and 2; CAA30245). 
Sequence information
Length: 509 AA [This is the length of the unprocessed precursor] Molecular weight: 58215 Da [This is the MW of the unprocessed precursor] CRC64: E622FAB351BC40AF [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSVSALSSTR FTGSISGFLQ VASVLGLLLL LVKAVQFYLQ RQWLLKAFQQ FPSPPFHWFF 

        70         80         90        100        110        120 
GHKQFQGDKE LQQIMTCVEN FPSAFPRWFW GSKAYLIVYD PDYMKVILGR SDPKANGVYR 

       130        140        150        160        170        180 
LLAPWIGYGL LLLNGQPWFQ HRRMLTPAFH YDILKPYVKN MADSIRLMLD KWEQLAGQDS 

       190        200        210        220        230        240 
SIEIFQHISL MTLDTVMKCA FSHNGSVQVD GNYKSYIQAI GNLNDLFHSR VRNIFHQNDT 

       250        260        270        280        290        300 
IYNFSSNGHL FNRACQLAHD HTDGVIKLRK DQLQNAGELE KVKKKRRLDF LDILLLARME 

       310        320        330        340        350        360 
NGDSLSDKDL RAEVDTFMFE GHDTTASGVS WIFYALATHP EHQQRCREEV QSVLGDGSSI 

       370        380        390        400        410        420 
TWDHLDQIPY TTMCIKEALR LYPPVPGIVR ELSTSVTFPD GRSLPKGIQV TLSIYGLHHN 

       430        440        450        460        470        480 
PKVWPNPEVF DPSRFAPDSP RHSHSFLPFS GGARNCIGKQ FAMSEMKVIV ALTLLRFELL 

       490        500 
PDPTKVPIPL PRLVLKSKNG IYLYLKKLH 

P08516 in FASTA format

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