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UniProtKB/Swiss-Prot entry P08065


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DHSA_BACSU
Primary accession number P08065
Secondary accession numbers None
Integrated into Swiss-Prot on August 1, 1988
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    September 2, 2008 (Entry version 80)
Name and origin of the protein
Protein name Succinate dehydrogenase flavoprotein subunit
Synonym EC 1.3.99.1
Gene name
Name: sdhA
Synonyms: citF
OrderedLocusNames: BSU28440
From
Bacillus subtilis [TaxID: 1423] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 3: Inferred from homology;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=168 / PY79;
PubMed=3027051 [NCBI, ExPASy, EBI, Israel, Japan]
Phillips M.K., Hederstedt L., Hasnain S., Rutberg L., Guest J.R.;
"Nucleotide sequence encoding the flavoprotein and iron-sulfur protein subunits of the Bacillus subtilis PY79 succinate dehydrogenase complex.";
J. Bacteriol. 169:864-873(1987).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=168;
PubMed=8969504 [NCBI, ExPASy, EBI, Israel, Japan]
Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R.;
"The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism.";
Microbiology 142:3067-3078(1996).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=168;
DOI=10.1038/36786; PubMed=9384377 [NCBI, ExPASy, EBI, Israel, Japan]
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.;
"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.";
Nature 390:249-256(1997).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31.
STRAIN=168 / PY79;
PubMed=3086287 [NCBI, ExPASy, EBI, Israel, Japan]
Magnusson K., Philips M.K., Guest J.R., Rutberg L.;
"Nucleotide sequence of the gene for cytochrome b558 of the Bacillus subtilis succinate dehydrogenase complex.";
J. Bacteriol. 166:1067-1071(1986).
[5]
MUTANT ASP-48.
DOI=10.1021/bi00366a033; PubMed=3021212 [NCBI, ExPASy, EBI, Israel, Japan]
Maguire J.J., Magnusson K., Hederstedt L.;
"Bacillus subtilis mutant succinate dehydrogenase lacking covalently bound flavin: identification of the primary defect and studies on the iron-sulfur clusters in mutated and wild-type enzyme.";
Biochemistry 25:5202-5208(1986).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M13470; AAA22746.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z75208; CAA99547.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z99118; CAB14804.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A27763; A27763.
RefSeq NP_390722.1; -.
3D structure databases
HSSP P83223; 1D4D. [HSSP ENTRY / PDB]
ModBase P08065.
Enzyme and pathway databases
BioCyc BSUB224308:BSU2840-MON; -.
Organism-specific databases
SubtiList BG10352; sdhA. [Micado]
Family and domain databases
InterPro IPR003953; FAD_bind2_N.
IPR013027; FAD_pyr_nucl-diS_OxRdtase.
IPR003952; FRD_SDH_FAD_BS.
IPR004112; Fum_Rdtase/Succ_DHase_flav_C.
IPR001100; Pyr_nuc-diS_OxRdtase.
IPR011280; Succ_DHase/Fum_Rdtase_flav_su.
Graphical view of domain structure.
PANTHER PTHR11632:SF4; SDH_A_Bsu; 1.
Pfam PF00890; FAD_binding_2; 1.
PF02910; Succ_DH_flav_C; 1.
Pfam graphical view of domain structure.
PRINTS PR00368; FADPNR.
PR00411; PNDRDTASEI.
TIGRFAMs TIGR01811; sdhA_Bsu; 1.
PROSITE PS00504; FRD_SDH_FAD_BINDING; 1.
BLOCKS P08065.
Genome annotation databases
GeneID 937600; -.
GenomeReviews AL009126_GR; BSU28440.
KEGG bsu:BSU28440; -.
NMPDR fig|224308.1.peg.2847; -.
Phylogenomic databases
HOGENOM P08065; -.
Genome annotation databases
CMR P08065; BSU28440.
Other
ProtoNet P08065.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cell membrane; Complete proteome; Electron transport; FAD; Flavoprotein; Membrane; Oxidoreductase; Transport; Tricarboxylic acid cycle.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   586  585     Succinate dehydrogenase flavoprotein subunit. PRO_0000158650
NP_BIND   33    48  16     FAD (By similarity). 
ACT_SITE   285   285        Proton acceptor (By similarity). 
BINDING   236   236        Substrate (By similarity). 
BINDING   250   250        Substrate (By similarity). 
BINDING   352   352        Substrate (By similarity). 
BINDING   386   386        Substrate (By similarity). 
MOD_RES   41    41        Tele-8alpha-FAD histidine (By similarity). 
VARIANT   48    48  1     G -> D (in a defective mutant). 
Sequence information
Length: 586 AA [This is the length of the unprocessed precursor] Molecular weight: 65350 Da [This is the MW of the unprocessed precursor] CRC64: 355B8C890E9F768D [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSQSSIIVVG GGLAGLMATI KAAESGMAVK LFSIVPVKRS HSVCAQGGIN GAVNTKGEGD 

        70         80         90        100        110        120 
SPWEHFDDTV YGGDFLANQP PLKAMCEAAP SIIHLLDRMA VMFNRTPEGL LDFRRFGGTQ 

       130        140        150        160        170        180 
HHRTAYAGAT TGQQLLYALD EQVRRYEVAG LVTKYEGWEF LGAVLDDDRT CRGIVAQNLT 

       190        200        210        220        230        240 
NMQIESFRSD AVIMATGGPG IIFGKSTNSM INTGSAASIV YQQGAYYANG EFIQIHPTAI 

       250        260        270        280        290        300 
PGDDKLRLMS ESARGEGGRV WTYKDGKPWY FLEEKYPAYG NLVPRDIATR EIFDVCVNQK 

       310        320        330        340        350        360 
LGINGENMVY LDLSHKDPKE LDIKLGGIIE IYEKFMGDDP RKLPMKIFPA VHYSMGGLWV 

       370        380        390        400        410        420 
DYDQMTNIPG LFAAGECDYS MHGGNRLERT SLLSAIYGGM VAGPNAVKYV NGLESSAEDM 

       430        440        450        460        470        480 
SSSLFDAHVK KEEEKWADIM IMDGTENAYV LHKELGEWMT ANVTVVRHND KLLKTDDKIQ 

       490        500        510        520        530        540 
ELMERFKKIN INDTTKWSNQ GAMFTRQFSN MLQLARVITL GAYNRNESRG AHYKPDYPER 

       550        560        570        580 
NDDEWLKTTM AKHVSPYEAP EFEYQDVDVS LITPRKRDYS KKKVAK 

P08065 in FASTA format

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