[1]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=Sprague-Dawley; TISSUE=Liver; DOI=10.1093/nar/16.15.7746; PubMed=3412913 [NCBI, ExPASy, EBI, Israel, Japan] Ho Y., Howard A.J., Crapo J.D.; "Cloning and sequence of a cDNA encoding rat glucose-6-phosphate dehydrogenase."; Nucleic Acids Res. 16:7746-7746(1988).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Kidney; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[3]	PROTEIN SEQUENCE OF 2-514. STRAIN=Wistar; TISSUE=Liver; PubMed=2606104 [NCBI, ExPASy, EBI, Israel, Japan] Jeffery J., Barros-Soederling J., Murray L., Wood I., Hansen R., Szepesi B., Joernvall H.; "Glucose-6-phosphate dehydrogenase. Characteristics revealed by the rat liver enzyme structure."; Eur. J. Biochem. 186:551-556(1989).
[4]	NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 41-515. Fritz R.S., Kletzien R.F.; Submitted (NOV-1989) to the EMBL/GenBank/DDBJ databases.
[5]	PROTEIN SEQUENCE OF 58-72; 176-192 AND 228-257, AND MASS SPECTROMETRY. STRAIN=Sprague-Dawley; TISSUE=Spinal cord; Lubec G., Afjehi-Sadat L.; Submitted (NOV-2006) to UniProtKB.

Comments

CATALYTIC ACTIVITY: D-glucose 6-phosphate + NADP⁺ = D-glucono-1,5-lactone 6-phosphate + NADPH.
PATHWAY: Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3 .
MISCELLANEOUS: Has NADP both as cofactor (bound to the N-terminal domain) and as structural element bound to the C-terminal domain.
SIMILARITY: Belongs to the glucose-6-phosphate dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X07467; CAA30355.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC081820; AAH81820.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M26655; AAA41179.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M26653; AAA41179.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M26654; AAA41179.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

S01233; S01233.

NP_058702.1; -.

3D structure databases

HSSP

P11413; 1QKI. [HSSP ENTRY / PDB]

SMR

P05370; 28-515.

ModBase

P05370.

Organism-specific databases

RGD

2645; G6pdx.

Gene expression databases

ArrayExpress

P05370; -.

GermOnline

ENSRNOG00000037254; Rattus norvegicus.

Family and domain databases

InterPro

IPR001282; Glc-6-P_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR23429; G6PDH; 1.

Pfam

PF02781; G6PD_C; 1.
PF00479; G6PD_N; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000110; G6PD; 1.

PRINTS

PR00079; G6PDHDRGNASE.

ProDom

PD001129; G6PD; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGRFAMs

TIGR00871; zwf; 1.

PROSITE

PS00069; G6P_DEHYDROGENASE; 1.

BLOCKS

P05370.

Genome annotation databases

Ensembl

ENSRNOG00000037254; Rattus norvegicus. [Contig view]

GeneID

24377; -.

KEGG

rno:24377; -.

Phylogenomic databases

HOVERGEN

P05370; -.

Other

ProtoNet

P05370.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Acetylation; Carbohydrate metabolism; Direct protein sequencing; Glucose metabolism; NADP; Oxidoreductase; Phosphoprotein.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 515`	`514`	`Glucose-6-phosphate 1-dehydrogenase.`	`PRO_0000068087`
`ACT_SITE`	`263 263`		`Proton acceptor (By similarity).`
`BINDING`	`40 40`		`NADP (By similarity).`
`BINDING`	`72 72`		`NADP (By similarity).`
`BINDING`	`201 201`		`Substrate (By similarity).`
`BINDING`	`205 205`		`Substrate (By similarity).`
`BINDING`	`238 238`		`NADP (By similarity).`
`BINDING`	`357 357`		`NADP (By similarity).`
`BINDING`	`487 487`		`NADP (By similarity).`
`MOD_RES`	`2 2`		`N-acetylalanine.`
`MOD_RES`	`401 401`		`Phosphotyrosine (By similarity).`
`MOD_RES`	`503 503`		`Phosphotyrosine (By similarity).`
`MOD_RES`	`507 507`		`Phosphotyrosine (By similarity).`
`CONFLICT`	`320 320`		`N -> D (in Ref. 3; AA sequence).`

Sequence information

Length: 515 AA [This is the length of the unprocessed precursor]

Molecular weight: 59376 Da [This is the MW of the unprocessed precursor]

CRC64: 8E92FFF3BF5A959B [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAEQVALSRT QVCGILREEL YQGDAFHQAD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL 

        70         80         90        100        110        120 
LPEDTFIVGY ARSRLTVDDI RKQSEPFFKV TPEERPKLEE FFARNSYVAG QYDDPASYKH 

       130        140        150        160        170        180 
LNSHMNALHQ GMQANRLFYL ALPPTVYEAV TKNIQEICMS QTGWNRIIVE KPFGRDLQSS 

       190        200        210        220        230        240 
NQLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP 

       250        260        270        280        290        300 
FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPASTD SDDVRDEKVK VLKCISEVET 

       310        320        330        340        350        360 
DNVVLGQYVG NPSGEGEATN GYLDDPTVPH GSTTATFAAA VLYVENERWD GVPFILRCGK 

       370        380        390        400        410        420 
ALNERKAEVR LQFRDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL 

       430        440        450        460        470        480 
DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH KIDREKPQPI 

       490        500        510 
PYVYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL

P05370 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools