ID   CASA1_SHEEP             Reviewed;         214 AA.
AC   P04653; Q69B23; Q9TS03; Q9TS48;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 3.
DT   22-JUL-2008, entry version 60.
DE   RecName: Full=Alpha-S1-casein;
DE   Flags: Precursor;
GN   Name=CSN1S1;
OS   Ovis aries (Sheep).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Caprinae; Ovis.
OX   NCBI_TaxID=9940;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (C ALLELE; ISOFORM 2).
RX   MEDLINE=86104473; PubMed=3002501; DOI=10.1016/S0300-9084(85)80291-1;
RA   Mercier J.-C., Gaye P., Soulier S., Hue-Delahaie D., Vilotte J.-L.;
RT   "Construction and identification of recombinant plasmids carrying
RT   cDNAs coding for ovine alpha S1-, alpha S2-, beta-, kappa-casein and
RT   beta-lactoglobulin. Nucleotide sequence of alpha S1-casein cDNA.";
RL   Biochimie 67:959-971(1985).
RN   [2]
RP   PROTEIN SEQUENCE OF 16-214 (A; C AND D ALLELES; ISOFORMS 1 AND 2),
RP   PHOSPHORYLATION AT SER-27; SER-56; SER-61; SER-63; SER-79; SER-80;
RP   SER-81; SER-82; SER-83; SER-90 AND SER-130, AND MASS SPECTROMETRY.
RC   TISSUE=Milk;
RX   MEDLINE=95325458; PubMed=7601973;
RA   Ferranti P., Malorni A., Nitti G., Laezza P., Pizzano R., Chianese L.,
RA   Addeo F.;
RT   "Primary structure of ovine alpha s1-caseins: localization of
RT   phosphorylation sites and characterization of genetic variants A, C
RT   and D.";
RL   J. Dairy Res. 62:281-296(1995).
RN   [3]
RP   VARIANT THR-209.
RC   STRAIN=Comisana, Sarda, and Sopravissana; TISSUE=Blood;
RX   PubMed=15328285;
RA   Ceriotti G., Chessa S., Bolla P., Budelli E., Bianchi L., Duranti E.,
RA   Caroli A.;
RT   "Single nucleotide polymorphisms in the ovine casein genes detected by
RT   polymerase chain reaction-single strand conformation polymorphism.";
RL   J. Dairy Sci. 87:2606-2613(2004).
CC   -!- FUNCTION: Important role in the capacity of milk to transport
CC       calcium phosphate.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1; Synonyms=Long;
CC         IsoId=P04653-2; Sequence=Displayed;
CC       Name=2; Synonyms=Short;
CC         IsoId=P04653-1; Sequence=VSP_012408;
CC   -!- TISSUE SPECIFICITY: Mammary gland specific. Secreted in milk.
CC   -!- MASS SPECTROMETRY: Mass=23638.14; Mass_error=3.0;
CC       Method=Electrospray; Range=16-214 (P04653-2); Note=Allele A, with
CC       11 phosphate groups; Source=PubMed:7601973;
CC   -!- MASS SPECTROMETRY: Mass=22620.0; Mass_error=1.6;
CC       Method=Electrospray; Range=16-214 (P04653-1); Note=Allele A, with
CC       11 phosphate groups; Source=PubMed:7601973;
CC   -!- MASS SPECTROMETRY: Mass=23561.8; Mass_error=2.3;
CC       Method=Electrospray; Range=16-214 (P04653-2); Note=Allele C, with
CC       10 phosphate groups; Source=PubMed:7601973;
CC   -!- MASS SPECTROMETRY: Mass=22551.8; Mass_error=2.2;
CC       Method=Electrospray; Range=16-214 (P04653-1); Note=Allele C, with
CC       10 phosphate groups; Source=PubMed:7601973;
CC   -!- MASS SPECTROMETRY: Mass=23290.2; Mass_error=2.9;
CC       Method=Electrospray; Range=16-214 (P04653-2); Note=Allele D, with
CC       6 phosphate groups; Source=PubMed:7601973;
CC   -!- MASS SPECTROMETRY: Mass=22629.0; Mass_error=1.6;
CC       Method=Electrospray; Range=16-214 (P04653-1); Note=Allele D, with
CC       6 phosphate groups; Source=PubMed:7601973;
CC   -!- POLYMORPHISM: At least four alleles are known. The sequence shown
CC       is that of allele A. The frequency of variant Thr-209 is 0.263,
CC       0.122 and 0.191 in Italian breeds Comisana, Sarda and
CC       Sopravissana, respectively.
CC   -!- SIMILARITY: Belongs to the alpha-casein family.
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DR   EMBL; X03237; CAA26982.1; -; mRNA.
DR   EMBL; AY444506; AAS17944.1; -; Genomic_DNA.
DR   PIR; A25069; KASHS1.
DR   RefSeq; NP_001009795.1; -.
DR   UniGene; Oar.429; -.
DR   GeneID; 443382; -.
DR   HOVERGEN; P04653; -.
DR   InterPro; IPR001588; Casein.
DR   Pfam; PF00363; Casein; 1.
DR   PROSITE; PS00306; CASEIN_ALPHA_BETA; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Direct protein sequencing; Phosphoprotein;
KW   Polymorphism; Secreted; Signal.
FT   SIGNAL        1     15
FT   CHAIN        16    214       Alpha-S1-casein.
FT                                /FTId=PRO_0000004459.
FT   REGION      105    111       Opioid-like peptide sequence.
FT   MOD_RES      27     27       Phosphoserine; in allele A.
FT   MOD_RES      56     56       Phosphoserine; in allele C.
FT   MOD_RES      61     61       Phosphoserine.
FT   MOD_RES      63     63       Phosphoserine.
FT   MOD_RES      79     79       Phosphoserine; in alleles A and C.
FT   MOD_RES      80     80       Phosphoserine.
FT   MOD_RES      81     81       Phosphoserine; in alleles A and C.
FT   MOD_RES      82     82       Phosphoserine.
FT   MOD_RES      83     83       Phosphoserine; in alleles A and C.
FT   MOD_RES      90     90       Phosphoserine.
FT   MOD_RES     130    130       Phosphoserine.
FT   VAR_SEQ     156    163       Missing (in isoform 2).
FT                                /FTId=VSP_012408.
FT   VARIANT      28     28       S -> P (in allele C and allele D).
FT   VARIANT      83     83       S -> N (in allele D).
FT   VARIANT     209    209       I -> T.
SQ   SEQUENCE   214 AA;  24306 MW;  9312FBFFBD6E49DC CRC64;
     MKLLILTCLV AVALARPKHP IKHQGLSSEV LNENLLRFVV APFPEVFRKE NINELSKDIG
     SESIEDQAME DAKQMKAGSS SSSEEIVPNS AEQKYIQKED VPSERYLGYL EQLLRLKKYN
     VPQLEIVPKS AEEQLHSMKE GNPAHQKQPM IAVNQELAYF YPQLFRQFYQ LDAYPSGAWY
     YLPLGTQYTD APSFSDIPNP IGSENSGKIT MPLW
//