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UniProtKB/Swiss-Prot entry P04636

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MDHM_RAT
Primary accession number P04636
Secondary accession number Q6GSM4
Integrated into Swiss-Prot on August 13, 1987
Sequence was last modified on January 9, 2007 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 86)
Name and origin of the protein
Protein name Malate dehydrogenase, mitochondrial [Precursor]
Synonym EC 1.1.1.37
Gene name
Name: Mdh2
Synonyms: Mor1
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1093/nar/14.15.6053; PubMed=3755817 [NCBI, ExPASy, EBI, Israel, Japan]
Grant P.M., Tellam J., May V.L., Strauss A.W.;
"Isolation and nucleotide sequence of a cDNA clone encoding rat mitochondrial malate dehydrogenase.";
Nucleic Acids Res. 14:6053-6066(1986).
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Pituitary anterior lobe;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[3]
 PROTEIN SEQUENCE OF 25-338.
DOI=10.1021/bi00375a019; PubMed=3828294 [NCBI, ExPASy, EBI, Israel, Japan]
Grant P.M., Roderick S.L., Grant G.A., Banaszak L.J., Strauss A.W.;
"Comparison of the precursor and mature forms of rat heart mitochondrial malate dehydrogenase.";
Biochemistry 26:128-134(1987).
[4]
 PROTEIN SEQUENCE OF 27-52; 53-74; 166-185; 192-203; 216-239; 242-296 AND 308-324, AND MASS SPECTROMETRY.
STRAIN=Sprague-Dawley;
TISSUE=Brain, Hippocampus, and Spinal cord;
Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U.;
Submitted (JUL-2007) to UniProtKB.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X04240; CAA27812.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC063165; AAH63165.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A25509; DERTMM.
RefSeq NP_112413.2; -.
UniGene Rn.1011
3D structure databases
HSSP P00346; 1MLD. [HSSP ENTRY / PDB]
SMR P04636; 25-337.
ModBase P04636.
2D gel databases
Rat-heart-2DPAGE P04636; -.
Organism-specific databases
RGD 619719; Mor1.
Gene expression databases
ArrayExpress P04636; -.
GermOnline ENSRNOG00000001440; Rattus norvegicus.
Ontologies
GO
GO:0005759; Cellular component: mitochondrial matrix (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005488; Molecular function: binding (inferred from electronic annotation from InterPro).
GO:0030060; Molecular function: L-malate dehydrogenase activity (inferred from electronic annotation from InterPro).
GO:0006096; Biological process: glycolysis (inferred from electronic annotation from InterPro).
GO:0006108; Biological process: malate metabolic process (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0006099; Biological process: tricarboxylic acid cycle (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001557; L-lactate/malate_DHase.
IPR001236; Lactate/malate_DHase.
IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
IPR001252; Malate_DHase_AS.
IPR010097; Malate_DHase_NAD-dep_euk_g_bac.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.90.110.10; lact_mal_DH; 1.
G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR11540:SF1; MDH_euk_g_bac; 1.
Pfam PF02866; Ldh_1_C; 1.
PF00056; Ldh_1_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000102; Lac_mal_DH; 1.
TIGRFAMs TIGR01772; MDH_euk_gproteo; 1.
PROSITE PS00068; MDH; 1.
ProtoNet P04636.
Genome annotation databases
Ensembl ENSRNOG00000001440; Rattus norvegicus. [Contig view]
GeneID 81829; -.
KEGG rno:81829; -.
NMPDR fig|10116.3.peg.8382; -.
Phylogenomic databases
HOVERGEN P04636; -.
Other
NextBio 615775; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Acetylation; Direct protein sequencing; Mitochondrion; NAD; Oxidoreductase; Phosphoprotein; Transit peptide; Tricarboxylic acid cycle.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    24  24     Mitochondrion. 
CHAIN   25   338  314     Malate dehydrogenase, mitochondrial. PRO_0000018631
NP_BIND   31    37  7     NAD (By similarity). 
NP_BIND   140   142  3     NAD (By similarity). 
ACT_SITE   200   200        Proton acceptor (By similarity). 
BINDING   57    57        NAD (By similarity). 
BINDING   104   104        Substrate (By similarity). 
BINDING   110   110        Substrate (By similarity). 
BINDING   117   117        NAD (By similarity). 
BINDING   142   142        Substrate (By similarity). 
BINDING   176   176        Substrate (By similarity). 
BINDING   251   251        NAD (By similarity). 
MOD_RES   56    56        Phosphotyrosine (By similarity). 
MOD_RES   157   157        N6-acetyllysine (By similarity). 
MOD_RES   239   239        N6-acetyllysine (By similarity). 
MOD_RES   314   314        N6-acetyllysine (By similarity). 
CONFLICT   229   229        R -> K (in Ref. 1; CAA27812). 
Sequence information
Length: 338 AA [This is the length of the unprocessed precursor] Molecular weight: 35684 Da [This is the MW of the unprocessed precursor] CRC64: 13849CCFB78C87E7 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MLSALARPVG AALRRSFSTS AQNNAKVAVL GASGGIGQPL SLLLKNSPLV SRLTLYDIAH 

        70         80         90        100        110        120 
TPGVAADLSH IETRANVKGY LGPEQLPDCL KGCDVVVIPA GVPRKPGMTR DDLFNTNATI 

       130        140        150        160        170        180 
VATLTAACAQ HCPEAMICII SNPVNSTIPI TAEVFKKHGV YNPNKIFGVT TLDIVRANTF 

       190        200        210        220        230        240 
VAELKGLDPA RVNVPVIGGH AGKTIIPLIS QCTPKVDFPQ DQLATLTGRI QEAGTEVVKA 

       250        260        270        280        290        300 
KAGAGSATLS MAYAGARFVF SLVDAMNGKE GVIECSFVQS KETECTYFST PLLLGKKGLE 

       310        320        330 
KNLGIGKITP FEEKMIAEAI PELKASIKKG EDFVKNMK
P04636 in FASTA format

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