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UniProtKB/Swiss-Prot entry P04298

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MCEL_VACCV
Primary accession number P04298
Secondary accession number Q76ZS6
Integrated into Swiss-Prot on March 20, 1987
Sequence was last modified on March 20, 1987 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 53)
Name and origin of the protein
Protein name mRNA-capping enzyme large subunit
Synonyms None
Includes Polynucleotide 5'-triphosphatase
     (EC 3.1.3.33)
     (mRNA 5'-triphosphatase)
     (TPase)
mRNA guanylyltransferase
     (EC 2.7.7.50)
     (GTP--RNA guanylyltransferase)
     (GTase)
Gene name
OrderedLocusNames: VACWR106
ORFNames: D1R
From
Vaccinia virus (strain Western Reserve / WR) (VACV) [TaxID: 10254] 
Taxonomy Viruses; dsDNA viruses, no RNA stage; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
Virus host Homo sapiens (Human) [TaxID: 9606]
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0042-6822(86)90011-5; PubMed=3739227 [NCBI, ExPASy, EBI, Israel, Japan]
Niles E.G., Condit R.C., Caro P., Davidson K., Matusick L., Seto J.;
"Nucleotide sequence and genetic map of the 16-kb vaccinia virus HindIII D fragment.";
Virology 153:96-112(1986).
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.;
"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb.";
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
[3]
 INTERACTION WITH SMALL SUBUNIT, AND PROTEIN SEQUENCE OF 1-20 AND 498-517.
PubMed=2161527 [NCBI, ExPASy, EBI, Israel, Japan]
Guo P., Moss B.;
"Interaction and mutual stabilization of the two subunits of vaccinia virus mRNA capping enzyme coexpressed in Escherichia coli.";
Proc. Natl. Acad. Sci. U.S.A. 87:4023-4027(1990).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M15058; AAA48253.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY243312; AAO89385.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A03872; QQVZ1.
RefSeq YP_232988.1; -.
3D structure databases
PDB
2VDW; X-ray; 2.70 A; A/C/E/G=545-844.[ExPASy / RCSB / EBI]
PDBsum 2VDW; -.
ModBase P04298.
Ontologies
GO
GO:0004484; Molecular function: mRNA guanylyltransferase activity (inferred from electronic annotation from EC).
GO:0004651; Molecular function: polynucleotide 5'-phosphatase activity (inferred from electronic annotation from EC).
GO:0006370; Biological process: mRNA capping (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR004971; Pox_MCEL.
Graphical view of domain structure.
Pfam PF03291; Pox_MCEL; 1.
Pfam graphical view of domain structure.
ProtoNet P04298.
Genome annotation databases
GeneID 3707562; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Direct protein sequencing; Hydrolase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotidyltransferase; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   844  844     mRNA-capping enzyme large subunit. PRO_0000210124
ACT_SITE   260   260        N6-GMP-lysine intermediate (Potential). 
HELIX   563   580  18      
TURN   583   585  3      
STRAND   593   596  4      
TURN   600   604  5      
HELIX   605   610  6      
STRAND   614   621  8      
HELIX   623   636  14      
STRAND   646   651  6      
STRAND   656   658  3      
HELIX   659   664  6      
STRAND   672   679  8      
HELIX   681   683  3      
TURN   687   689  3      
HELIX   690   700  11      
STRAND   701   712  12      
HELIX   714   717  4      
STRAND   724   727  4      
STRAND   730   732  3      
TURN   734   736  3      
STRAND   737   741  5      
STRAND   743   745  3      
STRAND   748   752  5      
TURN   754   756  3      
STRAND   757   759  3      
STRAND   761   764  4      
HELIX   768   777  10      
STRAND   780   787  8      
HELIX   788   793  6      
HELIX   796   800  5      
HELIX   802   805  4      
HELIX   809   823  15      
HELIX   829   833  5      
STRAND   836   843  8      
Sequence information
Length: 844 AA [This is the length of the unprocessed precursor] Molecular weight: 96734 Da [This is the MW of the unprocessed precursor] CRC64: 8B9FD3DE836FA6E7 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MDANVVSSST IATYIDALAK NASELEQRST AYEINNELEL VFIKPPLITL TNVVNISTIQ 

        70         80         90        100        110        120 
ESFIRFTVTN KEGVKIRTKI PLSKVHGLDV KNVQLVDAID NIVWEKKSLV TENRLHKECL 

       130        140        150        160        170        180 
LRLSTEERHI FLDYKKYGSS IRLELVNLIQ AKTKNFTIDF KLKYFLGSGA QSKSSLLHAI 

       190        200        210        220        230        240 
NHPKSRPNTS LEIEFTPRDN ETVPYDELIK ELTTLSRHIF MASPENVILS PPINAPIKTF 

       250        260        270        280        290        300 
MLPKQDIVGL DLENLYAVTK TDGIPITIRV TSNGLYCYFT HLGYIIRYPV KRIIDSEVVV 

       310        320        330        340        350        360 
FGEAVKDKNW TVYLIKLIEP VNAINDRLEE SKYVESKLVD ICDRIVFKSK KYEGPFTTTS 

       370        380        390        400        410        420 
EVVDMLSTYL PKQPEGVILF YSKGPKSNID FKIKKENTID QTANVVFRYM SSEPIIFGES 

       430        440        450        460        470        480 
SIFVEYKKFS NDKGFPKEYG SGKIVLYNGV NYLNNIYCLE YINTHNEVGI KSVVVPIKFI 

       490        500        510        520        530        540 
AEFLVNGEIL KPRIDKTMKY INSEDYYGNQ HNIIVEHLRD QSIKIGDIFN EDKLSDVGHQ 

       550        560        570        580        590        600 
YANNDKFRLN PEVSYFTNKR TRGPLGILSN YVKTLLISMY CSKTFLDDSN KRKVLAIDFG 

       610        620        630        640        650        660 
NGADLEKYFY GEIALLVATD PDADAIARGN ERYNKLNSGI KTKYYKFDYI QETIRSDTFV 

       670        680        690        700        710        720 
SSVREVFYFG KFNIIDWQFA IHYSFHPRHY ATVMNNLSEL TASGGKVLIT TMDGDKLSKL 

       730        740        750        760        770        780 
TDKKTFIIHK NLPSSENYMS VEKIADDRIV VYNPSTMSTP MTEYIIKKND IVRVFNEYGF 

       790        800        810        820        830        840 
VLVDNVDFAT IIERSKKFIN GASTMEDRPS TRNFFELNRG AIKCEGLDVE DLLSYYVVYV 


FSKR
P04298 in FASTA format

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