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UniProtKB/Swiss-Prot entry P00365


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DHE2_NEUCR
Primary accession number P00365
Secondary accession numbers Q02222 Q7RUZ7
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on June 7, 2005 (Sequence version 4)
Annotations were last modified on    September 2, 2008 (Entry version 70)
Name and origin of the protein
Protein name NAD-specific glutamate dehydrogenase
Synonyms NAD-GDH
EC 1.4.1.2
Gene name
Name: gdh-1
ORFNames: NCU00461
From
Neurospora crassa [TaxID: 5141] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=8398079 [NCBI, ExPASy, EBI, Israel, Japan]
Kapoor M., Vijayaraghavan Y., Kadonaga R., LaRue K.E.;
"NAD(+)-specific glutamate dehydrogenase of Neurospora crassa: cloning, complete nucleotide sequence, and gene mapping.";
Biochem. Cell Biol. 71:205-219(1993).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
DOI=10.1038/nature01554; PubMed=12712197 [NCBI, ExPASy, EBI, Israel, Japan]
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
"The genome sequence of the filamentous fungus Neurospora crassa.";
Nature 422:859-868(2003).
[3]
PROTEIN SEQUENCE OF 1-44 AND 47-353.
PubMed=6447150 [NCBI, ExPASy, EBI, Israel, Japan]
Haberland M.E., Smith E.L.;
"Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of Neurospora crassa. Isolation and sequences of several cyanogen bromide peptides from the NH2-terminal portion of the peptide chain.";
J. Biol. Chem. 255:7984-7992(1980).
[4]
PROTEIN SEQUENCE OF 381-1050.
PubMed=21191 [NCBI, ExPASy, EBI, Israel, Japan]
Austen B.M., Haberland M.E., Nyc J.F., Smith E.L.;
"Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of Neurospora. IV. The COOH-terminal 669 residues of the peptide chain; comparison with other glutamate dehydrogenases.";
J. Biol. Chem. 252:8142-8149(1977).
[5]
NUCLEOTIDE SEQUENCE [MRNA] OF 642-948.
PubMed=2521336 [NCBI, ExPASy, EBI, Israel, Japan]
Vierula P.J., Kapoor M.;
"NAD-specific glutamate dehydrogenase of Neurospora crassa. cDNA cloning and gene expression during derepression.";
J. Biol. Chem. 264:1108-1114(1989).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
S66039; AAB28355.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AABX02000001; EAA27544.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M23436; AAA33601.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A92284; DENCED.
T46599; T46599.
RefSeq XP_956780.1; -.
3D structure databases
ModBase P00365.
Family and domain databases
InterPro IPR006095; Glu/Leu/Phe/Val_DHase.
IPR006096; Glu/Leu/Phe/Val_DHase_C.
IPR016210; Glu_DHase_NAD-dep.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF00208; ELFV_dehydrog; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000184; GDH_NAD; 1.
PROSITE PS00074; GLFV_DEHYDROGENASE; 1.
BLOCKS P00365.
Genome annotation databases
GeneID 3872927; -.
KEGG ncr:NCU00461; -.
NMPDR fig|5141.1.peg.480; -.
Other
ProtoNet P00365.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Direct protein sequencing; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom    To Length Description FTId
CHAIN   1   1050  1050     NAD-specific glutamate dehydrogenase. PRO_0000182732
ACT_SITE   594    594        By similarity. 
CONFLICT   3      5        SPS -> APD (in Ref. 3; AA sequence). 
CONFLICT   11     11        H -> R (in Ref. 3; AA sequence). 
CONFLICT   31     41        HVSYPKVNGNG -> VPYSKVDGGN (in Ref. 3; AA sequence). 
CONFLICT   44     44        V -> Q (in Ref. 3; AA sequence). 
CONFLICT   113    114        TS -> ST (in Ref. 3; AA sequence). 
CONFLICT   153    153        T -> TTN (in Ref. 3; AA sequence). 
CONFLICT   154    154        Missing (in Ref. 1; AAB28355). 
CONFLICT   216    216        P -> PEGDP (in Ref. 3; AA sequence). 
CONFLICT   345    347        LPQ -> PQL (in Ref. 3; AA sequence). 
CONFLICT   351    352        HN -> NH (in Ref. 3; AA sequence). 
CONFLICT   461    464        EVLS -> SEVL (in Ref. 4; AA sequence). 
CONFLICT   637    637        L -> V (in Ref. 1; AAB28355). 
CONFLICT   660    660        T -> I (in Ref. 1; AAB28355). 
CONFLICT   708    709        Missing (in Ref. 1; AAB28355). 
CONFLICT   753    753        N -> D (in Ref. 4; AA sequence). 
CONFLICT   788    788        D -> N (in Ref. 5; AAA33601). 
CONFLICT   799    801        VVH -> HV (in Ref. 4; AA sequence). 
CONFLICT   845    846        ST -> TS (in Ref. 4; AA sequence). 
CONFLICT   909    909        D -> N (in Ref. 4; AA sequence). 
CONFLICT   1024   1024        V -> VE (in Ref. 4; AA sequence). 
CONFLICT   1035   1035        Missing (in Ref. 4; AA sequence). 
CONFLICT   1038   1039        DF -> AD (in Ref. 4; AA sequence). 
CONFLICT   1041   1041        S -> T (in Ref. 1; AAB28355). 
Sequence information
Length: 1050 AA [This is the length of the unprocessed precursor] Molecular weight: 118584 Da [This is the MW of the unprocessed precursor] CRC64: CDCACCFD221D2CB3 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MDSPSAPVPA HKLVDRLKDQ TPRHPSPQPT HVSYPKVNGN GHRVLRSATV GYVAPVFQGK 

        70         80         90        100        110        120 
AEQMKQVKNI IVQGGWIPET LVDGQIAWFY NELGIDDVYF QLENPQAVAN HITSLYAAKV 

       130        140        150        160        170        180 
AAFSREDKRE EIRLDMEASD HAIYIDTSEP GMTSFDGPRY EHRLESKYLD GDDTSKRFRV 

       190        200        210        220        230        240 
ETFRSPGVLG QKENSKAALR CYFVYQCLFV DSNADPKETR LEVISDRMFL AKATKNTKQI 

       250        260        270        280        290        300 
YQDIIQVAVS RHGPVIEVFD IEGSEEMRLV VAFRSRTAKG IFSALSDLYH YYGVTSSRKY 

       310        320        330        340        350        360 
VEQFSNGITV MSIYLRPAAN IDGKHPPLEQ SIHQITKEIS LLYCLPQNKF HNMFASGELS 

       370        380        390        400        410        420 
LQETIYAHCV WVFVQHFLNR LGTEYTSLIA ALDPKNNSHV EILSKMKKRL RTETFTPDYI 

       430        440        450        460        470        480 
LEIISSHPQL VRALYASFAS VHLRVGSDYD RHLIAPTPVM EVLSDARLKE KITKDVSNEH 

       490        500        510        520        530        540 
EEMVMTAFRV FNNAVLKTNF FTPTKVALSF RLNPSFLPEV EYPKPLYGMF LVITSESRGF 

       550        560        570        580        590        600 
HLRFKDIARG GIRIVKSRSK EAYQINARNL FDENYGLAST QQRKNKDIPE GGSKGVILLD 

       610        620        630        640        650        660 
PKQQDRHREA FEKYIDSILD LLLKAETPGI KNPIVDLYGK EEILFMGPDE NTADLVDWAT 

       670        680        690        700        710        720 
EHARARGAPW WKSFFTGKSP RLGGIPHDSY GMTTLSVREY VKGIYRKLEL DPSKIRKMQT 

       730        740        750        760        770        780 
GGPDGDLGSN EILLSNETYT AIVDGSGVLC DPNGIDKDEL RRLAKARAMI SNFDIAKLSK 

       790        800        810        820        830        840 
DGYRVLCDDT NVTLPNGEVV HNGTAFRNTY HLRDNGITDM FVPCGGRPES IDLSSVNKLI 

       850        860        870        880        890        900 
KDGKSTIPYI VEGANLFITQ DAKLRLEEAG CIVYKDASAN KGGVTSSSLE VLASLSFDDK 

       910        920        930        940        950        960 
GFVTHMCHDS RGNAPEFYQA YVKEVQNKIQ DNARLEFEAI WREHEQTGLP RSVLSDKLSL 

       970        980        990       1000       1010       1020 
AITSLDEDLQ RSELWDNEKI RRSVLADALP NLLINKIGLD TIIERVPDSY LRAIFGSYLA 

      1030       1040       1050 
SRFVYEFGSS PSQFAFYDFM SKRMGNINKE 

P00365 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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