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UniProtKB/Swiss-Prot entry P00349


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name 6PGD_SHEEP
Primary accession number P00349
Secondary accession numbers None
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on January 23, 2007 (Sequence version 4)
Annotations were last modified on    July 22, 2008 (Entry version 83)
Name and origin of the protein
Protein name 6-phosphogluconate dehydrogenase, decarboxylating
Synonym EC 1.1.1.44
Gene name
Name: PGD
From
Ovis aries (Sheep) [TaxID: 9940] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae; Caprinae; Ovis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Liver;
PubMed=1471978 [NCBI, ExPASy, EBI, Israel, Japan]
Somers D.O., Medd S.M., Walker J.E., Adams M.J.;
"Sheep 6-phosphogluconate dehydrogenase. Revised protein sequence based upon the sequences of cDNA clones obtained with the polymerase chain reaction.";
Biochem. J. 288:1061-1067(1992).
[2]
PRELIMINARY PROTEIN SEQUENCE OF 2-483.
TISSUE=Liver;
PubMed=6685125 [NCBI, ExPASy, EBI, Israel, Japan]
Carne A., Walker J.E.;
"Amino acid sequence of ovine 6-phosphogluconate dehydrogenase.";
J. Biol. Chem. 258:12895-12906(1983).
[3]
X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
PubMed=6641716 [NCBI, ExPASy, EBI, Israel, Japan]
Adams M.J., Archibald I.G., Bugg C.E., Carne A., Gover S., Helliwell J.R., Pickersgill R.W., White S.W.;
"The three dimensional structure of sheep liver 6-phosphogluconate dehydrogenase at 2.6-A resolution.";
EMBO J. 2:1009-1014(1983).
[4]
X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
DOI=10.1107/S0108768191010315; PubMed=1793548 [NCBI, ExPASy, EBI, Israel, Japan]
Adams M.J., Gover S., Leaback R., Phillips C., Somers D.O.;
"The structure of 6-phosphogluconate dehydrogenase refined at 2.5-A resolution.";
Acta Crystallogr. B 47:817-820(1991).
[5]
X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
DOI=10.1016/S0969-2126(00)00066-6; PubMed=7922042 [NCBI, ExPASy, EBI, Israel, Japan]
Adams M.J., Ellis G.H., Gover S., Naylor C.E., Phillips C.;
"Crystallographic study of coenzyme, coenzyme analogue and substrate binding in 6-phosphogluconate dehydrogenase: implications for NADP specificity and the enzyme mechanism.";
Structure 2:651-668(1994).
[6]
X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
DOI=10.1107/S0907444994012229; PubMed=15299295 [NCBI, ExPASy, EBI, Israel, Japan]
Phillips C., Gover S., Adams M.J.;
"Structure of 6-phosphogluconate dehydrogenase refined at 2-A resolution.";
Acta Crystallogr. D 51:290-304(1995).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X60195; CAA42751.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S15280; S15280.
S27359; DESHGC.
RefSeq NP_001009467.1; -.
UniGene Oar.419
3D structure databases
PDB
1PGN; X-ray; 2.30 A; A=1-483.[ExPASy / RCSB / EBI]
1PGO; X-ray; 2.50 A; A=1-483.[ExPASy / RCSB / EBI]
1PGP; X-ray; 2.50 A; A=1-483.[ExPASy / RCSB / EBI]
1PGQ; X-ray; 3.17 A; A=1-483.[ExPASy / RCSB / EBI]
2PGD; X-ray; 2.00 A; A=1-483.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1PGN; -.
1PGO; -.
1PGP; -.
1PGQ; -.
2PGD; -.
ModBase P00349.
Family and domain databases
InterPro IPR006183; 6-phosphogluconate_DHase.
IPR006114; 6PGDH_C.
IPR006113; 6PGDH_decarbox.
IPR006115; 6PGDH_NAD-bd.
IPR006184; 6PGdom_BS.
IPR013328; DHase_multihelical.
IPR012284; Fibritin/6PGD_C-extension.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:1.20.5.320; Fibritin/6PGD_C-extension; 1.
G3DSA:3.40.50.720; NAD(P)-bd; 1.
G3DSA:1.10.1040.10; Opine_DH; 1.
Pfam PF00393; 6PGD; 1.
PF03446; NAD_binding_2; 1.
Pfam graphical view of domain structure.
PRINTS PR00076; 6PGDHDRGNASE.
TIGRFAMs TIGR00873; gnd; 1.
PROSITE PS00461; 6PGD; 1.
BLOCKS P00349.
Genome annotation databases
GeneID 443541; -.
Phylogenomic databases
HOVERGEN P00349; -.
Other
LinkHub P00349; -.
ProtoNet P00349.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Direct protein sequencing; NADP; Oxidoreductase; Pentose shunt.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   483  482     6-phosphogluconate dehydrogenase, decarboxylating. PRO_0000090066
STRAND   4     9  6      
HELIX   13    24  12      
STRAND   29    32  4      
HELIX   37    44  8      
TURN   45    49  5      
HELIX   58    64  7      
STRAND   70    73  4      
HELIX   79    91  13      
STRAND   97   100  4      
HELIX   106   118  13      
STRAND   122   130  9      
HELIX   131   137  7      
STRAND   140   145  6      
HELIX   147   149  3      
HELIX   150   160  11      
TURN   165   167  3      
STRAND   168   170  3      
HELIX   179   207  29      
HELIX   213   223  11      
TURN   224   228  5      
HELIX   231   241  11      
STRAND   247   250  4      
HELIX   251   253  3      
HELIX   263   274  12      
HELIX   279   292  14      
HELIX   294   303  10      
HELIX   316   349  34      
HELIX   355   361  7      
STRAND   364   366  3      
HELIX   372   382  11      
HELIX   389   391  3      
HELIX   393   416  24      
HELIX   421   434  14      
HELIX   440   451  12      
STRAND   457   460  4      
Sequence information
Length: 483 AA [This is the length of the unprocessed precursor] Molecular weight: 52970 Da [This is the MW of the unprocessed precursor] CRC64: F4AC5DB414385F11 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAQADIALIG LAVMGQNLIL NMNDHGFVVC AFNRTVSKVD DFLANEAKGT KVLGAHSLEE 

        70         80         90        100        110        120 
MVSKLKKPRR IILLVKAGQA VDNFIEKLVP LLDIGDIIID GGNSEYRDTM RRCRDLKDKG 

       130        140        150        160        170        180 
ILFVGSGVSG GEDGARYGPS LMPGGNKEAW PHIKAIFQGI AAKVGTGEPC CDWVGDDGAG 

       190        200        210        220        230        240 
HFVKMVHNGI EYGDMQLICE AYHLMKDVLG LGHKEMAKAF EEWNKTELDS FLIEITASIL 

       250        260        270        280        290        300 
KFQDADGKHL LPKIRDSAGQ KGTGKWTAIS ALEYGVPVTL IGEAVFARCL SSLKDERIQA 

       310        320        330        340        350        360 
SKKLKGPQNI PFEGDKKSFL EDIRKALYAS KIISYAQGFM LLRQAATEFG WTLNYGGIAL 

       370        380        390        400        410        420 
MWRGGCIIRS VFLGKIKDAF DRNPGLQNLL LDDFFKSAVE NCQDSWRRAI STGVQAGIPM 

       430        440        450        460        470        480 
PCFTTALSFY DGYRHAMLPA NLIQAQRDYF GAHTYELLAK PGQFIHTNWT GHGGSVSSSS 


YNA 

P00349 in FASTA format

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