NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13 AND 295-327, CHARACTERIZATION, AND MASS SPECTROMETRY.
STRAIN=ATCC 12755 / DSM 4841 / NCFB 2725;
DOI=10.1074/jbc.273.37.23812; PubMed=9726992 [NCBI, ExPASy, EBI, Israel, Japan]
Parsonage D., Luba J., Mallett T.C., Claiborne A.;
"The soluble alpha-glycerophosphate oxidase from Enterococcus casseliflavus. Sequence homology with the membrane-associated dehydrogenase and kinetic analysis of the recombinant enzyme.";
J. Biol. Chem. 273:23812-23822(1998).

Comments

CATALYTIC ACTIVITY: sn-glycerol 3-phosphate + O₂ = glycerone phosphate + H₂O₂.
COFACTOR: FAD.
PATHWAY: Membrane lipid metabolism; glycerophospholipid metabolism .
SUBCELLULAR LOCATION: Cytoplasm.
MASS SPECTROMETRY: Mass=67082; Method=Electrospray; Range=2-609; Source=PubMed:9726992;.
SIMILARITY: Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U57498; AAC34739.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

O86963.

Family and domain databases

InterPro

IPR000447; FAD-dep_Gly3P_DHase.
IPR006076; FAD-dep_OxRdtase.
Graphical view of domain structure.

Pfam

PF01266; DAO; 1.
Pfam graphical view of domain structure.

PRINTS

PR01001; FADG3PDH.

PROSITE

PS00977; FAD_G3PDH_1; 1.
PS00978; FAD_G3PDH_2; FALSE_NEG.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; Direct protein sequencing; FAD; Flavoprotein; Glycerol metabolism; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 609`	`608`	`Alpha-glycerophosphate oxidase.`	`PRO_0000126107`
`NP_BIND`	`21 49`	`29`	`FAD (Potential).`

Sequence information

Length: 609 AA [This is the length of the unprocessed precursor]

Molecular weight: 67216 Da [This is the MW of the unprocessed precursor]

CRC64: 38A17281C16990F6 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTFSQKDRKE TIQETAKTTY DVLIIGGGIT GAGVAVQTAA AGMKTVLLEM QDFAEGTSSR 

        70         80         90        100        110        120 
STKLVHGGIR YLKTFDVEVV ADTVRERAIV QQIAPHIPKP DPMLLPIYDE PGATFSLFSV 

       130        140        150        160        170        180 
KVAMDLYDRL ANVTGSKYEN YLLTKEEVLA REPQLQAENL VGGGVYLDFR NNDARLVIEN 

       190        200        210        220        230        240 
IKRAQADGAA MISKAKVVGI LHDEQGIING VEVEDQLTNE RFEVHAKVVI NTTGPWSDIV 

       250        260        270        280        290        300 
RQLDKNDELP PQMRPTKGVH LVVDREKLKV PQPTYFDTGK NDGRMVFVVP RENKTYFGTT 

       310        320        330        340        350        360 
DTDYTGDFAH PTVTQEDVDY LLTIVNERFP HAQITLDDIE ASWAGLRPLI TNNGGSDYNG 

       370        380        390        400        410        420 
GGKGKLSDES FEQIVESVKE YLADERQRPV VEKAVKQAQE RVEASKVDPS QVSRGSSLER 

       430        440        450        460        470        480 
SKDGLLTLAG GKITDYRLMA EGAVKRINEL LQESGASFEL VDSTTYPVSG GELDAANVEE 

       490        500        510        520        530        540 
ELAKLADQAQ TAGFNEAAAT YLAHLYGSNL PQVLNYKTKF EGLDEKESTA LNYSLHEEMV 

       550        560        570        580        590        600 
LTPVDYLLRR TNHILFMRDT LDDVKAGVVA AMTDFFGWSE EEKAAHVLEL NQVIAESDLT 


ALKGGKKDE

O86963 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools