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UniProtKB/Swiss-Prot entry O85064

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LEU3_BUCAP
Primary accession number O85064
Secondary accession numbers None
Integrated into Swiss-Prot on October 19, 2002
Sequence was last modified on November 1, 1998 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 46)
Name and origin of the protein
Protein name 3-isopropylmalate dehydrogenase
Synonyms EC 1.1.1.85
Beta-IPM dehydrogenase
IMDH
3-IPM-DH
Gene name
Name: leuB
OrderedLocusNames: BUsg_PL5
From
Buchnera aphidicola subsp. Schizaphis graminum [TaxID: 98794] [HAMAP proteome]
Encoded on Plasmid pLeu-Sg (pBSg1).
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Buchnera.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1007/PL00006447; PubMed=9873079 [NCBI, ExPASy, EBI, Israel, Japan]
Baumann L., Baumann P., Moran N.A., Sandstroem J.P., Thao M.L.;
"Genetic characterization of plasmids containing genes encoding enzymes of leucine biosynthesis in endosymbionts (Buchnera) of aphids.";
J. Mol. Evol. 48:77-85(1999).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF041836; AAD12594.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_047181.1; -.
3D structure databases
HSSP P37412; 1CNZ. [HSSP ENTRY / PDB]
ModBase O85064.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0003862; Molecular function: 3-isopropylmalate dehydrogenase activity (inferred from electronic annotation from HAMAP).
GO:0009098; Biological process: leucine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.
Family and domain databases
HAMAP MF_01033; -; 1.
PBIL [Tree]
InterPro IPR004429; 3-isopropylmalate_DHase.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.718.10; IDH_IMDH; 1.
PANTHER PTHR11835; IDH_IMDH_dimeric; 1.
PTHR11835:SF13; IPMDH; 1.
Pfam PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00169; leuB; 1.
PROSITE PS00470; IDH_IMDH; 1.
BLOCKS O85064.
Genome annotation databases
GeneID 2648309; -.
GenomeReviews AF041836_GR; BUsg_PL5.
Phylogenomic databases
HOGENOM O85064; -.
Genome annotation databases
CMR O85064; BUsg_PL5.
Other
ProtoNet O85064.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Cytoplasm; Leucine biosynthesis; Magnesium; Manganese; Metal-binding; NAD; Oxidoreductase; Plasmid.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   363  363     3-isopropylmalate dehydrogenase. PRO_0000083661
NP_BIND   78    91  14     NAD (By similarity). 
NP_BIND   285   297  13     NAD (By similarity). 
METAL   227   227        Magnesium or manganese (By similarity). 
METAL   251   251        Magnesium or manganese (By similarity). 
METAL   255   255        Magnesium or manganese (By similarity). 
BINDING   99    99        Substrate (By similarity). 
BINDING   109   109        Substrate (By similarity). 
BINDING   138   138        Substrate (By similarity). 
BINDING   227   227        Substrate (By similarity). 
SITE   145   145  1     Important for catalysis (By similarity). 
SITE   195   195  1     Important for catalysis (By similarity). 
Sequence information
Length: 363 AA [This is the length of the unprocessed precursor] Molecular weight: 40918 Da [This is the MW of the unprocessed precursor] CRC64: 1B70FDAEF9EC7D60 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MKKKFRIAVL PGDGIGPEVM REAYKILNIL ERKFFLKLEL REFDIGGIAI DREGIALPEK 

        70         80         90        100        110        120 
TLRGCENSDA ILFGSVGGKK WDYLPIESRP ERASLLPLRK HFNLFSNLRP AKLRVDLKNL 

       130        140        150        160        170        180 
SPLRSDIVRD GFDILCIREL TGGIYFGKPK GRFKNKNGEY AFDTEIYYDF EIERIANLAF 

       190        200        210        220        230        240 
ELASSRRCKV CSIDKANVLE SSVLWREIVK KVSKNYPDVH LSHLYIDNAC MQIIKNPTQF 

       250        260        270        280        290        300 
DILLCSNIFG DIISDECAMI TGSIGMLPSA SLNNQKFGLY EPAGGSAPDI EGKNIANPIA 

       310        320        330        340        350        360 
QILSLSMLIR YSMNLNEIAD KIDLSVHYAL KEGYRTLDIS DGKNYIKTNE MGDIIAEFLD 


NGK
O85064 in FASTA format

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