[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B). DOI=10.1021/bi991691i; PubMed=10677231 [NCBI, ExPASy, EBI, Israel, Japan] Weiss C., Zeng Y., Huang J., Sobocka M.B., Rushbrook J.I.; "Bovine NAD+-dependent isocitrate dehydrogenase: alternative splicing and tissue-dependent expression of subunit 1."; Biochemistry 39:1807-1816(2000).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-385 (ISOFORM B). STRAIN=Hereford; TISSUE=Ascending colon; NIH - Mammalian Gene Collection (MGC) project; Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

Comments

CATALYTIC ACTIVITY: Isocitrate + NAD⁺ = 2-oxoglutarate + CO₂ + NADH.
COFACTOR: Binds 1 magnesium or manganese ion per subunit (By similarity).
SUBUNIT: Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 (By similarity).
SUBCELLULAR LOCATION: Mitochondrion.
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name B

Isoform ID O77784-1

This is the isoform sequence displayed in this entry.

Name A

Isoform ID O77784-2

Features which should be applied to build the isoform sequence: VSP_002461.
TISSUE SPECIFICITY: Isoform A is predominant in heart muscle; also found in brain, kidney and liver. Isoform B is present in kidney and liver.
SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF090321; AAC83166.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF090322; AAC83167.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC104502; AAI04503.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

S58432; S58432.

XP_869155.1; -.

3D structure databases

HSSP

P39126; 1HQS. [HSSP ENTRY / PDB]

ModBase

O77784.

Family and domain databases

InterPro

IPR004434; IsoCit_DHase_NAD_mit.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.718.10; IDH_IMDH; 1.

PANTHER

PTHR11835; IDH_IMDH_dimeric; 1.

Pfam

PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00175; mito_nad_idh; 1.

PROSITE

PS00470; IDH_IMDH; 1.

BLOCKS

O77784.

Genome annotation databases

Ensembl

ENSBTAG00000018813; Bos taurus. [Contig view]

GeneID

613338; -.

KEGG

bta:613338; -.

Phylogenomic databases

HOVERGEN

O77784; -.

Other

ProtoNet

O77784.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Magnesium; Manganese; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Transit peptide; Tricarboxylic acid cycle.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 33`	`33`	`Mitochondrion (By similarity).`
`CHAIN`	`34 385`	`352`	`Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial.`	`PRO_0000014443`
`METAL`	`251 251`		`Magnesium or manganese (By similarity).`
`BINDING`	`133 133`		`Substrate (By similarity).`
`BINDING`	`164 164`		`Substrate (By similarity).`
`BINDING`	`251 251`		`Substrate (By similarity).`
`SITE`	`171 171`	`1`	`Critical for catalysis (By similarity).`
`SITE`	`218 218`	`1`	`Critical for catalysis (By similarity).`
`VAR_SEQ`	`361 385`		`RDMGGYSTTTDFIKSVIGHLHPYGG -> SDMGGYATCQDFTEAVIGALSNP (in isoform A).`	`VSP_002461`

Sequence information

Length: 385 AA [This is the length of the unprocessed precursor]

Molecular weight: 42497 Da [This is the MW of the unprocessed precursor]

CRC64: 8E974AD550F5D50D [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAALSRVRWL TRALVAAPNP GAWRSLCTST VAQASSRTQG EDVRVEGAFP VTMLPGDGVG 

        70         80         90        100        110        120 
PELMHAVKEV FKAASVPVEF QEHHLSEVQN MASEEKLEQV LSSMKENKVA IIGKIHTPME 

       130        140        150        160        170        180 
YKGELASYDM RLRRKLDLFA NVVHVKSLPG YKTRHNNLDL VIIREQTEGE YSSLEHESAR 

       190        200        210        220        230        240 
GVIECLKIVT RTKSQRIAKF AFDYATKKGR GKVTAVHKAN IMKLGDGLFL QCCEEVAELY 

       250        260        270        280        290        300 
PKIKFEKMII DNCCMQLVQN PYQFDVLVMP NLYGNIIDNL AAGLVGGAGV VPGESYSAEY 

       310        320        330        340        350        360 
AVFETGARHP FAQAVGRNIA NPTAMLLSAS NMLRHLNLEH HSNMIAEAVK KVIKVGKVRT 

       370        380 
RDMGGYSTTT DFIKSVIGHL HPYGG

O77784 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools