ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry O62699

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name NOS2_CANFA
Primary accession number O62699
Secondary accession number O97604
Integrated into Swiss-Prot on May 30, 2000
Sequence was last modified on September 13, 2005 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 67)
Name and origin of the protein
Protein name Nitric oxide synthase, inducible
Synonyms EC 1.14.13.39
Inducible NO synthase
Inducible NOS
iNOS
NOS type II
Gene name
Name: NOS2
From
Canis familiaris (Dog) [TaxID: 9615] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Aorta;
PubMed=9746458 [NCBI, ExPASy, EBI, Israel, Japan]
Wang X., McGregor C.G.A., Miller V.M.;
"Induction and cDNA sequence of inducible nitric oxide synthase from canine aortic smooth muscle cells.";
Am. J. Physiol. 275:H1122-H1129(1998).
[2]
 NUCLEOTIDE SEQUENCE [MRNA] OF 42-632.
TISSUE=Alveolar macrophage;
Haerter L., Straubinger R.K., Appel M.J.G.;
"The canine inducible NO synthase.";
Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF077821; AAC78630.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF032909; AAC15587.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_001003186.1; -.
UniGene Cfa.45041
3D structure databases
HSSP P29477; 1NOS. [HSSP ENTRY / PDB]
SMR O62699; 80-499.
ModBase O62699.
Ontologies
GO
GO:0005515; Molecular function: protein binding (inferred from sequence or structural similarity from UniProtKB).
GO:0042742; Biological process: defense response to bacterium (inferred from sequence or structural similarity from UniProtKB).
GO:0006801; Biological process: superoxide metabolic process (inferred from sequence or structural similarity from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR003097; FAD-binding_1.
IPR001094; Flavdoxin_like.
IPR008254; Flavodoxin/NO_synth.
IPR001709; FPN_cyt_redctse.
IPR004030; NO_synthase_oxygenase_reg.
IPR012144; NOS.
IPR001433; OxRdtase_FAD/NAD_bd.
Graphical view of domain structure.
Gene3D G3DSA:3.90.340.10; NO_synthase_oxygenase_reg; 1.
Pfam PF00667; FAD_binding_1; 1.
PF00258; Flavodoxin_1; 1.
PF00175; NAD_binding_1; 1.
PF02898; NO_synthase; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000333; NOS; 1.
PRINTS PR00369; FLAVODOXIN.
PR00371; FPNCR.
PROSITE PS51384; FAD_FR; 1.
PS50902; FLAVODOXIN_LIKE; 1.
PS60001; NOS; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS O62699.
Genome annotation databases
Ensembl ENSCAFG00000018642; Canis familiaris. [Contig view]
GeneID 403822; -.
KEGG cfa:403822; -.
Phylogenomic databases
HOVERGEN O62699; -.
Other
ProtoNet O62699.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Calcium; Calmodulin-binding; FAD; FMN; Heme; Iron; Metal-binding; NADP; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   1154  1154     Nitric oxide synthase, inducible. PRO_0000170927
DOMAIN   536    674  139     Flavodoxin-like. 
DOMAIN   727    967  241     FAD-binding FR-type. 
NP_BIND   620    651  32     FMN (By similarity). 
NP_BIND   764    775  12     FAD (By similarity). 
NP_BIND   900    910  11     FAD (By similarity). 
NP_BIND   975    993  19     NADP (By similarity). 
NP_BIND   1073   1088  16     NADP (By similarity). 
REGION   506    526  21     Calmodulin-binding (Potential). 
METAL   197    197        Iron (heme axial ligand) (By similarity). 
CONFLICT   42     52        DDLKNHKHHND -> KCHSLSKHRDE (in Ref. 2). 
CONFLICT   56     56        P -> S (in Ref. 2; AAC15587). 
CONFLICT   59     64        ETVQKL -> GTVKTS (in Ref. 2; AAC15587). 
CONFLICT   68     80        LDKLHATPLSRPQ -> TIKPAAPPLACPR (in Ref. 2). 
CONFLICT   91     92        MT -> RS (in Ref. 1; AAC78630). 
CONFLICT   102    104        KGD -> MGV (in Ref. 1; AAC78630). 
CONFLICT   108    108        K -> T (in Ref. 1; AAC78630). 
CONFLICT   111    115        SCLGA -> LCMGS (in Ref. 1; AAC78630). 
CONFLICT   119    119        P -> T (in Ref. 1; AAC78630). 
CONFLICT   125    127        EPR -> GPS (in Ref. 1; AAC78630). 
CONFLICT   133    134        PD -> TE (in Ref. 1; AAC78630). 
CONFLICT   149    149        S -> G (in Ref. 1; AAC78630). 
CONFLICT   169    169        E -> D (in Ref. 1; AAC78630). 
CONFLICT   394    395        RR -> SK (in Ref. 1; AAC78630). 
CONFLICT   497    499        VWQ -> LWL (in Ref. 1; AAC78630). 
CONFLICT   506    508        QRR -> HRK (in Ref. 1; AAC78630). 
CONFLICT   615    615        K -> N (in Ref. 1; AAC78630). 
CONFLICT   624    626        GSS -> RSN (in Ref. 1; AAC78630). 
Sequence information
Length: 1154 AA [This is the length of the unprocessed precursor] Molecular weight: 131847 Da [This is the MW of the unprocessed precursor] CRC64: D6AD3A88AE89E995 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MACPWKFLFR AKFHQYGMKE EKDINNNVEK PPGATPSPST QDDLKNHKHH NDSPQPLTET 

        70         80         90        100        110        120 
VQKLPESLDK LHATPLSRPQ HVRIKNWGNG MTFQDTLHHK AKGDLACKSK SCLGAIMNPK 

       130        140        150        160        170        180 
SLTREPRDKP TPPDELLPQA IEFVNQYYSS FKEAKIEEHL ARVEAVTKEI ETTGTYQLTG 

       190        200        210        220        230        240 
DELIFATKQA WRNAPRCIGR IQWSNLQVFD ARSCSTAKEM FEHICRHLRY ASNNGNIRSA 

       250        260        270        280        290        300 
ITVFPQRTDG KHDFRVWNAQ LIRYAGYQMP DGTILGDPAS VEFTQLCIDL GWKPKYGRFD 

       310        320        330        340        350        360 
VVPLVLQADG QDPEFFEIPP DLVLEVPMEH PKYEWFRELE LKWYALPAVA NMLLEVGGLE 

       370        380        390        400        410        420 
FPGCPFNGWY MGTEIGVRDF CDVQRYNILE EVGRRMGLET HKLASLWKDR AVIEINVAVL 

       430        440        450        460        470        480 
HSFQKQNVTI MDHHSAAESF MKYMQSEYRS RGGCPADWIW LVPPISGSIT PVFHQEMLNY 

       490        500        510        520        530        540 
VLSPFYYYQV EAWKTHVWQD EKRRPQRRKI QLKVLVKAVL FASMLMRKTM ASRVRVTILF 

       550        560        570        580        590        600 
ATETGKSETL ARDLGALFSC AFHPKVLCMD EYKLSHLEEE QLLLVVTSTF GNGDSPGNGE 

       610        620        630        640        650        660 
KLKKSLFMLK ELTNKFRYAV FGLGSSMYPQ FCAFAHDIDH KLSHLGASQL TPGGEGDELN 

       670        680        690        700        710        720 
GKEEAFRCWA VQTFKAACDT SDVRGKHCIQ IPRLYTSNVT WDPHHYRLLQ DSQPLDLNKA 

       730        740        750        760        770        780 
LSKMHAKNVF TLRLKSQRNL QSPISNRTTL QVELSCEDSQ ELSYLPGEHL GVFPGNQLAL 

       790        800        810        820        830        840 
VQGILERVVY SPAPLQPVHL ETLSERGSYW VRNNRLPPCS LSQALTYFLD ITTPPTHLLL 

       850        860        870        880        890        900 
RKLAQLAHQY AERHRLEILC HPSEYNKWKL TNSPTFLEVL EEFPSLRVSA GFLLSQLPIL 

       910        920        930        940        950        960 
KPRYYSISSS RDCTPMEVHL TVAVLVYPTR DGQGPLHHGV CSTWLSNLKP QDPVPCFVRS 

       970        980        990       1000       1010       1020 
AGNFKLPEDP SRPCILIGPG TGIAPFRSFW QQRLHDIKHK GLRGSRMTLV FGCRRPDEDH 

      1030       1040       1050       1060       1070       1080 
LYREEMLEMA QSGVLHEVHT AYSRLPGQPK VYVQDILRQQ LASQVLRMLH EEQGHLYVCG 

      1090       1100       1110       1120       1130       1140 
DVRMARDVAH TLKHLVAAKL SLSEEQVEDY FFQLKSQKRY HEDIFGAVFP YEVKKDGAAK 

      1150 
QPSDPRVPAA HGRS
O62699 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA) 		Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Hosted by kr flag YPRC Korea Mirror sites: Australia Brazil Canada China Switzerland
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!