[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION. DOI=10.1073/pnas.95.10.5700; PubMed=9576947 [NCBI, ExPASy, EBI, Israel, Japan] Luckhart S., Vodovotz Y., Cui L., Rosenberg R.; "The mosquito Anopheles stephensi limits malaria parasite development with inducible synthesis of nitric oxide."; Proc. Natl. Acad. Sci. U.S.A. 95:5700-5705(1998).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. DOI=10.1016/S0378-1119(99)00121-3; PubMed=10333518 [NCBI, ExPASy, EBI, Israel, Japan] Luckhart S., Rosenberg R.; "Gene structure and polymorphism of an invertebrate nitric oxide synthase gene."; Gene 232:25-34(1999).

Comments

FUNCTION: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Nitric oxide limits plasmodium development in the midgut.
CATALYTIC ACTIVITY: L-arginine + n NADPH + m O₂ = citrulline + nitric oxide + n NADP⁺.
COFACTOR: Heme group (By similarity).
COFACTOR: Binds 1 FAD (By similarity).
COFACTOR: Binds 1 FMN (By similarity).
ENZYME REGULATION: Stimulated by calcium/calmodulin (By similarity).
INDUCTION: Detected in the midgut and carcass soon after invasion of the midgut by plasmodium. Early induction is also primed by bacterial growth in the blood meal.
SIMILARITY: Belongs to the NOS family.
SIMILARITY: Contains 1 FAD-binding FR-type domain.
SIMILARITY: Contains 1 flavodoxin-like domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF130134; AAC68577.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF130124; AAC68577.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF130125; AAC68577.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF130126; AAC68577.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF130127; AAC68577.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF130128; AAC68577.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF130129; AAC68577.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF130130; AAC68577.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF130131; AAC68577.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF130132; AAC68577.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF130133; AAC68577.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T31331; T31331.

3D structure databases

HSSP

P29477; 1DD7. [HSSP ENTRY / PDB]

ModBase

O61608.

Family and domain databases

InterPro

IPR003097; FAD-binding_1.
IPR001094; Flavdoxin_like.
IPR008254; Flavodoxin/NO_synth.
IPR001709; FPN_cyt_redctse.
IPR004030; NO_synthase_oxygenase_reg.
IPR012144; NOS.
IPR001433; OxRdtase_FAD/NAD_bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.90.340.10; NO_synthase_oxygenase_reg; 1.

Pfam

PF00667; FAD_binding_1; 1.
PF00258; Flavodoxin_1; 1.
PF00175; NAD_binding_1; 1.
PF02898; NO_synthase; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000333; NOS; 1.

PRINTS

PR00369; FLAVODOXIN.
PR00371; FPNCR.

PROSITE

PS51384; FAD_FR; 1.
PS50902; FLAVODOXIN_LIKE; 1.
PS60001; NOS; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calmodulin-binding; FAD; FMN; Heme; Iron; Metal-binding; NADP; Oxidoreductase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1247`	`1247`	`Nitric oxide synthase.`	`PRO_0000170950`
`DOMAIN`	`567 766`	`200`	`Flavodoxin-like.`
`DOMAIN`	`795 1065`	`271`	`FAD-binding FR-type.`
`NP_BIND`	`712 743`	`32`	`FMN (By similarity).`
`NP_BIND`	`855 866`	`12`	`FAD (By similarity).`
`NP_BIND`	`998 1008`	`11`	`FAD (By similarity).`
`NP_BIND`	`1073 1091`	`19`	`NADP (By similarity).`
`NP_BIND`	`1170 1185`	`16`	`NADP (By similarity).`
`REGION`	`537 557`	`21`	`Calmodulin-binding (Potential).`
`COMPBIAS`	`51 54`	`4`	`Poly-Gly.`
`METAL`	`224 224`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 1247 AA [This is the length of the unprocessed precursor]

Molecular weight: 141651 Da [This is the MW of the unprocessed precursor]

CRC64: 5F435E5EAC3D3778 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MADTTTVVVE RREVAEGRES SKANHIGEER RGYDVSRKRC SISVHGGGTE GGGGNMRTNY 

        70         80         90        100        110        120 
RELSPASLRI HRKSSHDIRN TLLGPDGEVL HLHDPSGKGG DGMGKMPAVV KPIKLKSIVT 

       130        140        150        160        170        180 
KAESYDTMHG KASDVMSCSR EVCMGSVMTP HVIGTETRKP EIVQQHAKDF LDQYYSSIRR 

       190        200        210        220        230        240 
LKSPAHDSRW QQVQKEVEAT GSYHLTETEL IYGAKLAWRN SSRCIGRIQW SKLQVFDCRY 

       250        260        270        280        290        300 
VTTTSGMFEA ICNHIKYATN KGNLRSAITI FPQRTDGKHD YRIWNNQIIS YAGYKNADGK 

       310        320        330        340        350        360 
IIGDPANVEF TDFCVKLGWK SKRTEWDILP LVVSANGHDP DYFDYPPELI LEVPLSHPQF 

       370        380        390        400        410        420 
KWFAELNLRW YAVPMVSSML FDCGGIQFTA TAFSGWYMST EIGCRNLCDA NRRNLLEPIA 

       430        440        450        460        470        480 
IKMGLDTRNP TSLWKDKALV EINIAVLHSY QSRNITIVDH HTASESFMKH FENETKLRNG 

       490        500        510        520        530        540 
CPADWIWIVP PMSASVTPVF HQEMAVYYLR PSFEYQESAM KTHIWKKGRD SAKNKKPRRK 

       550        560        570        580        590        600 
FNFKQIARAV KFTSKLFGRA LSRRIKATVL YATETGRSEQ YARQLVELLG HAFNAQIYCM 

       610        620        630        640        650        660 
SDYDISSIEH EALLLVVAST FGNGDPPENG ELFAQDLYAM KLHESGHHQA HSELTIAASS 

       670        680        690        700        710        720 
KSFIKANSRS DLGKFGPMGG RKIDRLDSLR GSTTDTLSEE TFGPLSNVRF AVFALGSSAY 

       730        740        750        760        770        780 
PNFCAFGKYI DNILGELGGE RLMKMATGDE ICGQEQAFRK WAPEVFKIAC ETFCLDPEET 

       790        800        810        820        830        840 
LSDAAFALQS ELSENTVRYA PVAEYESLDR ALSKFHNKKS MECSVKRNPI NLHCEMNGTE 

       850        860        870        880        890        900 
RSTILVEIMA EGIDYEPGDH VGIFPANRKE IVDGIIERLT GVNDPDEMLQ LQVLKEKQTQ 

       910        920        930        940        950        960 
NGVYKSWEPH ERLPVCTLRT LLTRFLDITT PPTRQLLTYL ASCCGDKADE ERLLMLANES 

       970        980        990       1000       1010       1020 
SVYEDWRYWK LPHLLEVLEE FPSCRPPAAV FVAQLNALQP RFYSISSSPR KYSNEIHLTV 

      1030       1040       1050       1060       1070       1080 
AIVTYRAEDG EGAEHYGVCS NYLANLQSDD KIYLFVRSAP SFHMSKDRTK PVILIGPGTG 

      1090       1100       1110       1120       1130       1140 
IAPFRSFWQE WDHIKTEMVD CKIPKVWLFF GCRTKNVDLY RDEKEEMVQH GVLDRVFLAL 

      1150       1160       1170       1180       1190       1200 
SREENIPKTY VQDLALKEAE SISELIMQEK GHIYVCGDVT MAEHVYQTLR KILATREKRT 

      1210       1220       1230       1240 
ETEMEKYMLT LRDENRYHED IFGITLRTAE IHNKSRATAR IRMASQP

O61608 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools