Proline dehydrogenase
     (EC 1.5.99.8)
     (Proline oxidase)
Delta-1-pyrroline-5-carboxylate dehydrogenase
     (P5C dehydrogenase)
     (EC 1.5.1.12)

Gene name

Name:

putA

From

Klebsiella aerogenes

[TaxID: 28451]

Taxonomy

Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Klebsiella.

Protein existence

3: Inferred from homology;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=EM450; Surber M.W., Maloy S.; "DNA sequence analysis of the putA gene in Klebsiella aerogenes."; Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Oxidizes proline to glutamate for use as a carbon and nitrogen source and also function as a transcriptional repressor of the put operon (By similarity).
CATALYTIC ACTIVITY: L-proline + acceptor + H₂O = (S)-1-pyrroline-5-carboxylate + reduced acceptor.
CATALYTIC ACTIVITY: (S)-1-pyrroline-5-carboxylate + NAD(P)⁺ + 2 H₂O = L-glutamate + NAD(P)H.
COFACTOR: FAD.
PATHWAY: Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2 .
PATHWAY: Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2 .
SIMILARITY: In the N-terminal section; belongs to the proline dehydrogenase family.
SIMILARITY: In the C-terminal section; belongs to the aldehyde dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF038838; AAB95478.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P09546; 1K87. [HSSP ENTRY / PDB]

SMR

O52485; 2-49, 87-612.

ModBase

O52485.

Family and domain databases

InterPro

IPR016160; Ald_DHase_CS.
IPR016162; Ald_DHase_N.
IPR015590; Aldehyde_DHase.
IPR005933; d-1-pyrroline-5-COlate_DHase-3.
IPR002872; Proline_DHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.

PANTHER

PTHR11699; Aldehyde_dehyd; 1.

Pfam

PF00171; Aldedh; 1.
PF01619; Pro_dh; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01238; D1pyr5carbox3; 1.

PROSITE

PS00070; ALDEHYDE_DEHYDR_CYS; 1.
PS00687; ALDEHYDE_DEHYDR_GLU; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

DNA-binding; FAD; Flavoprotein; Multifunctional enzyme; NAD; Oxidoreductase; Proline metabolism; Repressor; Transcription; Transcription regulation.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1312`	`1312`	`Bifunctional protein putA.`	`PRO_0000056525`
`REGION`	`228 574`	`347`	`Proline dehydrogenase.`
`REGION`	`653 1119`	`467`	`Aldehyde dehydrogenase.`
`ACT_SITE`	`883 883`		`By similarity.`
`ACT_SITE`	`917 917`		`By similarity.`

Sequence information

Length: 1312 AA [This is the length of the unprocessed precursor]

Molecular weight: 143857 Da [This is the MW of the unprocessed precursor]

CRC64: BAF3CA7FC5D18599 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MGTTTMGVKL DDATRERIKS AASRIDRTPH WLIKQAIFNY LEKLENDETL PELPALLSGA 

        70         80         90        100        110        120 
ANESDDASEP TEEPYQPFLE FAEQILPQSV RRAAITAAWR RPETDAVPML LEQARLPQPL 

       130        140        150        160        170        180 
GEQAHKLAYQ LAEKLRNQKT ASGRAGMVQS LLQEFSLSSQ EGVALMCLAE ALLRIPDKAT 

       190        200        210        220        230        240 
RDALIRDKIS NGNWQSHIGR SPSLFVNAAT WGLLFTGKLV STHNETSLSR SLNRIIGKSG 

       250        260        270        280        290        300 
EPLIRKGVDM AMRLMGEQFV TGETIAEALA NARKLEEKGF RYSYDMLGEA ALTAADAQAY 

       310        320        330        340        350        360 
MVSYQQAIHA IGKASNGRGI YEGPGISIKL SALHPRYSRA QYDRVMEELY PRLKSLTLLA 

       370        380        390        400        410        420 
RQYDIGINID AEEADRLEIS LDLLEKLCFE PELAGWNGIG FVIQAYQKRC PFVIDYLIDL 

       430        440        450        460        470        480 
ATRSRRRLMI RLVKGAYWDS EIKRAQMEGL EGYPVYTRKV YTDVSYLACA KKLLAVPNLI 

       490        500        510        520        530        540 
YPQFATHNAH TLAAIYQLAG QNYYPGQYEF QCLHGMGEPL YEQVVGKVAD GKLNRPCRIY 

       550        560        570        580        590        600 
APVGTHETLL AYLVRRLLEN GANTSFVNRI ADNTLPLDEL VADPVSAVEK LAQQEGQAGL 

       610        620        630        640        650        660 
PHPKIPLPRD LYGSGRSNSA GLDLANEHRL ASLSSSLLNS ALHKWQALPM LEQPVAEGEM 

       670        680        690        700        710        720 
QPVVNPAEPK DIVGYVREAS DAEVQQALTS AINNAPIWFA TPPQERAAIL ERAAVLMESQ 

       730        740        750        760        770        780 
MPTLMGILVR EAGKTFSNAI AEVREAVDFL HYYAGQVRDD FDNETHRPLG PVVCISPWNF 

       790        800        810        820        830        840 
PLAIFTGQIA AALAAGNSVL AKPAEQTPLI AAQGVAILLE AGVPPGVIQL LPGRGETVGA 

       850        860        870        880        890        900 
ALTSDERVRG VMFTGSTEVA TLLQRNIASR LDPQGRPTPL IAETGGMNAM IVDSSALTEQ 

       910        920        930        940        950        960 
VVIDVLASAF DSAGQRCSAL RVLCLQEEVA DHTLTMLRGA MSECRMGNPG RLTTDIGPVI 

       970        980        990       1000       1010       1020 
DAEAKENIER HIQAMRAKGR TVYQAVRENS EDAREWRHGT FVPPTLIELD SFDELKKEVF 

      1030       1040       1050       1060       1070       1080 
GPVLHVVRYN RNELDKLVEQ INASGYGLTL GVHTRIDETI AQVTGSAKVG NLYVNRNMVG 

      1090       1100       1110       1120       1130       1140 
AVVGVQPFGG EGLSGTGPKA GGPLYLYRLL SSRPQDAVGV TFARQDAERP LDAQLKTLLE 

      1150       1160       1170       1180       1190       1200 
KPLQALQQWA AGRPELQALC QQYSEQAQSG TQRLLPGPTG ERNTLTLMPR ERVLCVADNE 

      1210       1220       1230       1240       1250       1260 
QDALIQLAAV LAVGCEVLWP DSALQRDLAK KLPREVSERI RFAKAEQLPV QAFDAVIYHG 

      1270       1280       1290       1300       1310 
DSDQLRELCE QVAARDGAIV SVQGFARGET NLLLERLYIE RSLSVNTAAA GA

O52485 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools