NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Microglia;
PubMed=8900532 [NCBI, ExPASy, EBI, Israel, Japan]
Lane T.E., Buchmeier M.J., Watry D.D., Fox H.S.;
"Expression of inflammatory cytokines and inducible nitric oxide synthase in brains of SIV-infected rhesus monkeys: applications to HIV-induced central nervous system disease.";
Mol. Med. 2:27-37(1996).

Comments

FUNCTION: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. INOS and NO may contribute to simian immunodeficiency virus (SIV)-induced central nervous system disease.
CATALYTIC ACTIVITY: L-arginine + n NADPH + m O₂ = citrulline + nitric oxide + n NADP⁺.
COFACTOR: Heme group (By similarity).
COFACTOR: Binds 1 FAD (By similarity).
COFACTOR: Binds 1 FMN (By similarity).
COFACTOR: Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity).
ENZYME REGULATION: Regulated by calcium/calmodulin (By similarity).
SUBUNIT: Homodimer. Binds SLC9A3R1 (By similarity).
INDUCTION: By lipopolysaccharide (LPS).
SIMILARITY: Belongs to the NOS family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U31907; AAC39525.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

UniGene

Mmu.3443

3D structure databases

HSSP

P35228; 4NOS. [HSSP ENTRY / PDB]

SMR

O46660; 63-162.

ModBase

O46660.

Family and domain databases

InterPro

IPR004030; NO_synthase_oxygenase_reg.
Graphical view of domain structure.

Gene3D

G3DSA:3.90.340.10; NO_synthase_oxygenase_reg; 1.

Pfam

PF02898; NO_synthase; 1.
Pfam graphical view of domain structure.

PROSITE

PS60001; NOS; PARTIAL.

BLOCKS

O46660.

Phylogenomic databases

HOVERGEN

O46660; -.

Other

ProtoNet

O46660.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calcium; Calmodulin-binding; FAD; FMN; Heme; Iron; Metal-binding; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`<1 >162`	`>162`	`Nitric oxide synthase, inducible.`	`PRO_0000170933`
`NON_TER`	`1 1`
`NON_TER`	`162 162`

Sequence information

Length: 162 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 17813 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: 0457464D32287065 [This is a checksum on the sequence]

        10         20         30         40         50         60 
GKDINNNMEK AACATSSLVT QDDLQYHSLS KQQNESPQPL VGTGKKSPES LVKPDATPLS 

        70         80         90        100        110        120 
SPRHVRIKNW GSGMTFQDTL HHKAKGILTC RSKSCLGSIM TPKSLTRGPR DKPTPPDELL 

       130        140        150        160 
PQAIEFVNLS LEISVQDQIP PVCNDCQYYG SFKEAKIEEH LA

O46660 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools