NUCLEOTIDE SEQUENCE [GENOMIC DNA].
TISSUE=Ovarian follicle membrane;
PubMed=11133676 [NCBI, ExPASy, EBI, Israel, Japan]
Boerboom D., Sirois J.;
"Equine P450 cholesterol side-chain cleavage and 3 beta-hydroxysteroid dehydrogenase/delta(5)-delta(4) isomerase: molecular cloning and regulation of their messenger ribonucleic acids in equine follicles during the ovulatory process.";
Biol. Reprod. 64:206-215(2001).

Comments

FUNCTION: Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.
CATALYTIC ACTIVITY: Cholesterol + reduced adrenal ferredoxin + O₂ = pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin + H₂O.
COFACTOR: Heme group (By similarity).
PATHWAY: Lipid metabolism; C21-steroid hormone metabolism .
SUBCELLULAR LOCATION: Mitochondrion membrane.
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF031664; AAC04700.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001075990.1; -.

UniGene

Eca.13386

3D structure databases

HSSP

P00189; 1SCC. [HSSP ENTRY / PDB]

ModBase

O46515.

Ontologies

GO:0031966;	Cellular component: mitochondrial membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00463; EP450I.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

BLOCKS

O46515.

Genome annotation databases

GeneID

100034229; -.

Phylogenomic databases

HOVERGEN

O46515; -.

Other

ProtoNet

O46515.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cholesterol metabolism; Heme; Iron; Lipid metabolism; Membrane; Metal-binding; Mitochondrion; Monooxygenase; Oxidoreductase; Steroid metabolism; Steroidogenesis; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 39`	`39`	`Mitochondrion (By similarity).`
`CHAIN`	`40 520`	`481`	`Cytochrome P450 11A1, mitochondrial.`	`PRO_0000003584`
`METAL`	`461 461`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 520 AA [This is the length of the unprocessed precursor]

Molecular weight: 60314 Da [This is the MW of the unprocessed precursor]

CRC64: B8420BF587850192 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLVRGLPLRS VLVKGCQPLL SAPREGPGHP RVPTGEGAGM SSHSPRPFKE IPSPGDNGWI 

        70         80         90        100        110        120 
NLYHFWREKG PKKLHYHHFQ NFQKYGPIYR EKLGNVESVY IVDPEDVALL FKFEGPHPER 

       130        140        150        160        170        180 
FLIPPWTAYH QYFQKPVGVL FKSSDAWKKD RLALNPEVMA LESIKNFIPL LDPVSQDFVS 

       190        200        210        220        230        240 
LLHRRMEQQG SGKFSGPIIE DLFRFAFESI TNVIFGERQG MLDEIVDPEA QRFIDAVYKM 

       250        260        270        280        290        300 
FHTSVPMLSL PPDLFRLFRT KTWRDHVAAW DTVFSKAEQY TEKFYQDLKQ KRHFDSYPGI 

       310        320        330        340        350        360 
FYRLLASNKL PFKDIQANVT EMLAGGVDTT SMSLQWHLYE IARNLRVQEM LREEVLAARR 

       370        380        390        400        410        420 
QAQGDTSTMV QMVPLLKASI KETLRLHPIA VTLQRYPQND LVIRDYMIPA KTLVQVSIYT 

       430        440        450        460        470        480 
MGQDPTFFSN PRRFDPTRWL DKNKDLTHFR NLGFGWGVRQ CLGRRIAELE MTLFLIHILE 

       490        500        510        520 
NFRVEIQHLN DVDSTFGLIL IPEKPISFTF WPITRAPPQA

O46515 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools