[1]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Liver; Kojima M., Morozumi T., Hamasima N., Okamoto T.; "Cloning of a pig lanosterol 14-demethylase cDNA."; Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=10788789 [NCBI, ExPASy, EBI, Israel, Japan] Kojima M., Morozumi T., Onishi A., Mitsuhashi T.; "Structure of the pig sterol 14 alpha-demethylase (CYP51) gene and its expression in the testis and other tissues."; J. Biochem. 127:805-811(2000).

Comments

FUNCTION: Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol (By similarity).
CATALYTIC ACTIVITY: Obtusifoliol + 3 O₂ + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24²⁸-trien-3-beta-ol + formate + 3 NADP⁺ + 4 H₂O.
COFACTOR: Heme group (By similarity).
PATHWAY: Steroid metabolism; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6 .
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane (Potential). Microsome membrane (Potential).
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB009988; BAA24134.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AB042982; BAA96092.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

JC7243; JC7243.

NP_999597.1; -.

3D structure databases

HSSP

P77901; 1E9X. [HSSP ENTRY / PDB]

ModBase

O46420.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0008398;	Molecular function: sterol 14-demethylase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002403; Cyt_P450_E_grp-IV.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00465; EP450IV.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

BLOCKS

O46420.

Genome annotation databases

GeneID

403334; -.

KEGG

ssc:403334; -.

Phylogenomic databases

HOVERGEN

O46420; -.

Other

ProtoNet

O46420.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cholesterol biosynthesis; Endoplasmic reticulum; Heme; Iron; Lipid synthesis; Membrane; Metal-binding; Microsome; Monooxygenase; NADP; Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 503`	`503`	`Cytochrome P450 51A1.`	`PRO_0000051999`
`TRANSMEM`	`24 44`	`21`	`Potential.`
`METAL`	`449 449`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 503 AA [This is the length of the unprocessed precursor]

Molecular weight: 56867 Da [This is the MW of the unprocessed precursor]

CRC64: 0302949CE461AFD6 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVLLGLLQAG GSVLGQAMEQ VTGVNLLSSL LLACAFTLIL VYLFRQAIGH LAPLPAGAKS 

        70         80         90        100        110        120 
PPYIFSPIPF LGHAIAFGKS PIEFLENAYE KYGPVFSFTM VGKTFTYLLG SDAAALLFNS 

       130        140        150        160        170        180 
KNEDLNAEDV YSRLTTPVFG KGVAYDVPNP VFLEQKKMLK SGLNIAHFRQ HVSIIEKETK 

       190        200        210        220        230        240 
EYFQSWGESG ERNLFEALSE LIILTASHCL HGKEIRSQLN EKVAQLYADL DGGFSHAAWL 

       250        260        270        280        290        300 
LPGWLPLPSF RRRDRAHREI KNIFYKAIQK RRQSEEKIDD ILQTLLDSTY KDGRPLTDDE 

       310        320        330        340        350        360 
VAGMLIGLLL AGQHTSSTTS AWMGFFLARD KTLQEKCYLE QKTVCGEDLP PLTYDQLKDL 

       370        380        390        400        410        420 
NLLDRCIKET LRLRPPIMTM MRMAKTPQTV AGYTIPPGHQ VCVSPTVNQR LKDSWVERLD 

       430        440        450        460        470        480 
FNPDRYLQDN PASGEKFAYV PFGAGRHRCI GENFAYVQIK TIWSTMLRLY EFDLIDGYFP 

       490        500 
TVNYTTMIHT PENPVIRYKR RSK

O46420 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools