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UniProtKB/Swiss-Prot entry O35077

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GPDA_RAT
Primary accession number O35077
Secondary accession number Q5I0F4
Integrated into Swiss-Prot on July 15, 1998
Sequence was last modified on January 23, 2007 (Sequence version 4)
Annotations were last modified on    July 22, 2008 (Entry version 61)
Name and origin of the protein
Protein name Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic
Synonyms GPDH-C
GPD-C
EC 1.1.1.8
Gene name
Name: Gpd1
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=Wistar;
TISSUE=White adipose tissue;
DOI=10.1016/S0014-5793(97)00619-4; PubMed=9237667 [NCBI, ExPASy, EBI, Israel, Japan]
Daikoku T., Shinohara Y., Shima A., Yamazaki N., Terada H.;
"Dramatic enhancement of the specific expression of the heart-type fatty acid binding protein in rat brown adipose tissue by cold exposure.";
FEBS Lett. 410:383-386(1997).
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Kidney;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[3]
 PROTEIN SEQUENCE OF 6-48; 21-35; 70-101; 188-224 AND 241-270, AND MASS SPECTROMETRY.
STRAIN=Sprague-Dawley;
TISSUE=Hippocampus, and Spinal cord;
Lubec G., Afjehi-Sadat L., Chen W.-Q.;
Submitted (APR-2007) to UniProtKB.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AB002558; BAA21763.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC088396; AAH88396.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_071551.2; -.
UniGene Rn.44452
3D structure databases
HSSP P90551; 1EVY. [HSSP ENTRY / PDB]
ModBase O35077.
Organism-specific databases
RGD 621381; Gpd1.
Gene expression databases
ArrayExpress O35077; -.
GermOnline ENSRNOG00000019213; Rattus norvegicus.
Family and domain databases
InterPro IPR013328; DHase_multihelical.
IPR016040; NAD(P)-bd.
IPR017751; NAD-dep_Gly3P_DH_euk.
IPR006168; NAD-dep_Gly3P_DHase.
IPR011128; NAD-dep_Gly3P_DHase_N.
IPR006109; NAD_Gly3P_DHase_C.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
G3DSA:1.10.1040.10; Opine_DH; 1.
PANTHER PTHR11728; NAD_Gly3P_DH; 1.
Pfam PF07479; NAD_Gly3P_dh_C; 1.
PF01210; NAD_Gly3P_dh_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000114; Glycerol-3-P_dh; 1.
PRINTS PR00077; GPDHDRGNASE.
ProDom PD001278; NAD_Gly3P_C; 1.
[Domain structure / List of seq. sharing at least 1 domain]
PROSITE PS00957; NAD_G3PDH; 1.
BLOCKS O35077.
Genome annotation databases
Ensembl ENSRNOG00000019213; Rattus norvegicus. [Contig view]
GeneID 60666; -.
KEGG rno:60666; -.
NMPDR fig|10116.3.peg.27756; -.
Phylogenomic databases
HOVERGEN O35077; -.
Other
ProtoNet O35077.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed (By similarity). 
CHAIN   2   349  348     Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic. PRO_0000138082
NP_BIND   10    15  6     NAD (By similarity). 
REGION   269   270  2     Substrate binding (By similarity). 
ACT_SITE   204   204        Proton acceptor (Potential). 
BINDING   41    41        NAD (By similarity). 
BINDING   97    97        NAD (By similarity). 
BINDING   120   120        Substrate (By similarity). 
BINDING   153   153        NAD; via amide nitrogen (By similarity). 
BINDING   269   269        NAD (By similarity). 
BINDING   296   296        NAD; via amide nitrogen (By similarity). 
BINDING   298   298        NAD (By similarity). 
CONFLICT   179   179        E -> D (in Ref. 1; BAA21763). 
CONFLICT   238   238        F -> S (in Ref. 1; BAA21763). 
CONFLICT   338   338        F -> I (in Ref. 1; BAA21763). 
CONFLICT   340   340        C -> R (in Ref. 1; BAA21763). 
Sequence information
Length: 349 AA [This is the length of the unprocessed precursor] Molecular weight: 37453 Da [This is the MW of the unprocessed precursor] CRC64: 26208A55DC033A9A [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MAGKKVCIVG SGNWGSAIAK IVGSNASQLA HFDPRVTMWV FEEDIGGRKL TEIINTQHEN 

        70         80         90        100        110        120 
VKYLPGHKLP PNVVAVPDVV QAATGADILV FVVPHQFIGK ICDQLKGHLK ANTIGISLIK 

       130        140        150        160        170        180 
GIDEGPNGLK LISEVIGESL GIPMSVLMGA NIASEVAEEK FCETTIGCKD PAQGQLLKEL 

       190        200        210        220        230        240 
MQTPNFRITV VQEVDTVEIC GALKNIVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK 

       250        260        270        280        290        300 
LFCSGSVSSA TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE MLNGQKLQGP 

       310        320        330        340 
QTARELHSIL QHKGLVDKFP LFTAVYKVCY EGQPVGEFIC CLQNHPEHM
O35077 in FASTA format

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