NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-37, AND BIOPHYSICOCHEMICAL PROPERTIES.
STRAIN=ATCC 29267 / DSM 3873 / LMG 2657 / NCPPB 3004;
PubMed=9352901 [NCBI, ExPASy, EBI, Israel, Japan]
Yum D.-Y., Lee Y.-P., Pan J.-G.;
"Cloning and expression of a gene cluster encoding three subunits of membrane-bound gluconate dehydrogenase from Erwinia cypripedii ATCC 29267 in Escherichia coli.";
J. Bacteriol. 179:6566-6572(1997).

Comments

FUNCTION: Part of the heterotrimer that catalyzes the conversion of D-gluconate to 2-dehydro-D-gluconate. This subunit functions as the dehydrogenase.
CATALYTIC ACTIVITY: D-gluconate + acceptor = 2-dehydro-D-gluconate + reduced acceptor.
COFACTOR: FAD.
BIOPHYSICOCHEMICAL PROPERTIES:

pH dependence: Optimum pH is 5.0;
Temperature dependence: Optimum temperature is 30 degrees Celsius;
SUBUNIT: Heterotrimer.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Periplasmic side (Potential).
SIMILARITY: Belongs to the GMC oxidoreductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U97665; AAC45885.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

B38575; B38575.

3D structure databases

ModBase

O34214.

Family and domain databases

InterPro

IPR003953; FAD_bind2_N.
IPR000172; GMC_OxRdtase_N.
Graphical view of domain structure.

Pfam

PF00890; FAD_binding_2; 1.
PF00732; GMC_oxred_N; 1.
Pfam graphical view of domain structure.

PROSITE

PS00623; GMC_OXRED_1; FALSE_NEG.
PS00624; GMC_OXRED_2; FALSE_NEG.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Direct protein sequencing; FAD; Flavoprotein; Membrane; Oxidoreductase; Signal.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 22`	`22`
`CHAIN`	`23 615`	`593`	`Gluconate 2-dehydrogenase flavoprotein.`	`PRO_0000045854`

Sequence information

Length: 615 AA [This is the length of the unprocessed precursor]

Molecular weight: 67242 Da [This is the MW of the unprocessed precursor]

CRC64: B9E1A84FD035609A [This is a checksum on the sequence]

        10         20         30         40         50         60 
MERGERVSVP VSGYSRGEGV TVANELKKVD AVVVGFGWAG AIMAKELTEA GLNVVALERG 

        70         80         90        100        110        120 
PHRDTYPDGA YPQSIDELTY NIRKKLFQDL SKSTVTIRHD ASQTAVPYRQ LAAFLPGTGT 

       130        140        150        160        170        180 
GGAGLHWSGV HFRVDPVELN LRSHYEARYG KNFIPEGMTI QDFGVSYNEL EPFFDQAEKV 

       190        200        210        220        230        240 
FGTSGSAWTI KGKMIGKEKG GNFYAPDRSS DFPLPAQKRT YSAQLFAQAA ESVGYHPYDM 

       250        260        270        280        290        300 
PSANTSGPYT NTYGAQMGPC NFCGYCSGYA CYMYSKASPN VNILPALRQE PKFELRNNAY 

       310        320        330        340        350        360 
VLRVNLTGDK KRATGVTYLD GQGREVVQPA DLVILSAFQF HNVHLMLLSG IGQPYNPITN 

       370        380        390        400        410        420 
EGVVGRNFAY QNISTLKALF DKNTTTNPFI GAGGAGVAVD DFNADNFDHG PYGFVGGSPF 

       430        440        450        460        470        480 
WVNQAGTKPV SGLPTPKGTP NWGSQWKAAV ADTYNHHISM DAHGAHQSYR ANYLDLDPNY 

       490        500        510        520        530        540 
KNVYGQPLLR MTFDWQDNDI RMAQFMVGKM RKITEAMNPK MIIGGAKGPG THFDTTVYQT 

       550        560        570        580        590        600 
THMSGGAIMG EDPKTSAVNR YLQSWDVPNV FVPGASAFPQ GLGYNPTGMV AALTYWSAKA 

       610 
IREQYLKNPG PLVQA

O34214 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools