NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-30; 27-65 AND 229-247.
STRAIN=K24;
PubMed=9260969 [NCBI, ExPASy, EBI, Israel, Japan]
Kim S.I., Leem S.-H., Choi J.-S., Chung Y.H., Kim S., Park Y.-M., Park Y.K., Lee Y.N., Ha K.-S.;
"Cloning and characterization of two catA genes in Acinetobacter lwoffii K24.";
J. Bacteriol. 179:5226-5231(1997).

Comments

FUNCTION: Can cleave 4-methyl-, 4-chloro-, and 3-methoxycatechol at lower rates than catechol, but has no activity with 4-nitrocatechol or protocatechuic acid.
CATALYTIC ACTIVITY: Catechol + O₂ = cis,cis-muconate.
COFACTOR: Binds 1 Fe(3+) ion per subunit.
PATHWAY: Aromatic compound metabolism; beta-ketoadipate pathway; 5-oxo-4,5-dihydrofuran-2-acetate from catechol: step 1/3 .
SUBUNIT: Homodimer (Potential).
INDUCTION: By aniline.
SIMILARITY: Belongs to the intradiol ring-cleavage dioxygenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U77659; AAC31767.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T46825; T46825.

3D structure databases

HSSP

P07773; 1DMH. [HSSP ENTRY / PDB]

ModBase

O33950.

Family and domain databases

InterPro

IPR007535; Catechol_dOase_N.
IPR012801; Cchol_dOase_prob.
IPR000627; Intradiol_dOase_C.
IPR015889; Intradiol_dOase_core.
Graphical view of domain structure.

Gene3D

G3DSA:2.60.130.10; Intradiol_dOase_core; 1.

Pfam

PF00775; Dioxygenase_C; 1.
PF04444; Dioxygenase_N; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR02439; catechol_proteo; 1.

PROSITE

PS00083; INTRADIOL_DIOXYGENAS; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Aromatic hydrocarbons catabolism; Dioxygenase; Direct protein sequencing; Iron; Metal-binding; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 275`	`275`	`Catechol 1,2-dioxygenase 2.`	`PRO_0000085082`
`METAL`	`158 158`		`Iron (By similarity).`
`METAL`	`192 192`		`Iron (By similarity).`
`METAL`	`216 216`		`Iron (By similarity).`
`METAL`	`218 218`		`Iron (By similarity).`

Sequence information

Length: 275 AA [This is the length of the unprocessed precursor]

Molecular weight: 30399 Da [This is the MW of the unprocessed precursor]

CRC64: 3F8B0C127F45A807 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNKQAIDALL QKINDSAINE GNPRTKQIVN RIVRDLFYTI EDLDVQPDEF WTALNYLGDA 

        70         80         90        100        110        120 
GRSGELGLLA AGLGFEHFLD LRMDEAEAKA GVEGGTPRTI EGPLYVAGAP VSDGHARLDD 

       130        140        150        160        170        180 
GTDPGQTLVM RGRVFGEDGK PLANALVEVW HANHLGNYSY FDKSQPAFNL RRSIRTDAEG 

       190        200        210        220        230        240 
KYSFRSVVPV GYSVPPQGQT QLLLDQLGRH GHRPAHIHFF VSAPGFRKLT TQINIDGDPY 

       250        260        270 
LWDDFAFATR DGLVPAVRQA EVRKANRTAW TVSSR

O33950 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools