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UniProtKB/Swiss-Prot entry O29627


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LEU3_ARCFU
Primary accession number O29627
Secondary accession numbers None
Integrated into Swiss-Prot on May 30, 2000
Sequence was last modified on January 1, 1998 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 57)
Name and origin of the protein
Protein name 3-isopropylmalate dehydrogenase
Synonyms 3-IPM-DH
IMDH
EC 1.1.1.85
Beta-IPM dehydrogenase
Gene name
Name: leuB
OrderedLocusNames: AF_0628
From
Archaeoglobus fulgidus [TaxID: 2234] [HAMAP proteome]
Taxonomy Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae; Archaeoglobus.
Protein existence 3: Inferred from homology;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126;
DOI=10.1038/37052; PubMed=9389475 [NCBI, ExPASy, EBI, Israel, Japan]
Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G., Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus.";
Nature 390:364-370(1997).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE000782; AAB90611.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR D69328; D69328.
RefSeq NP_069462.1; -.
3D structure databases
HSSP P00351; 1XAA. [HSSP ENTRY / PDB]
ModBase O29627.
Enzyme and pathway databases
BioCyc AFUL224325:AF_0628-MON; -.
Family and domain databases
InterPro IPR001804; IsoCit_IM_DHase.
IPR011828; LEU3_arc.
Graphical view of domain structure.
Gene3D G3DSA:3.40.718.10; IDH_IMDH; 1.
PANTHER PTHR11835; IDH_IMDH_dimeric; 1.
Pfam PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR02088; LEU3_arch; 1.
PROSITE PS00470; IDH_IMDH; 1.
BLOCKS O29627.
Genome annotation databases
GeneID 1483846; -.
GenomeReviews AE000782_GR; AF_0628.
KEGG afu:AF0628; -.
NMPDR fig|224325.1.peg.621; -.
TIGR AF_0628; -.
Phylogenomic databases
HOGENOM O29627; -.
Other
ProtoNet O29627.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Cytoplasm; Leucine biosynthesis; Magnesium; Manganese; Metal-binding; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   326  326     3-isopropylmalate dehydrogenase. PRO_0000083808
NP_BIND   255   267  13     NAD (By similarity). 
METAL   198   198        Magnesium or manganese (By similarity). 
METAL   222   222        Magnesium or manganese (By similarity). 
METAL   226   226        Magnesium or manganese (By similarity). 
BINDING   81    81        Substrate (By similarity). 
BINDING   91    91        Substrate (By similarity). 
BINDING   112   112        Substrate (By similarity). 
BINDING   198   198        Substrate (By similarity). 
SITE   119   119  1     Important for catalysis (By similarity). 
SITE   165   165  1     Important for catalysis (By similarity). 
Sequence information
Length: 326 AA [This is the length of the unprocessed precursor] Molecular weight: 35819 Da [This is the MW of the unprocessed precursor] CRC64: 4B3BE727572CF0A9 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKKIVVIPGD GIGKEVMEAA MLILEKLDLP FEYSYYDAGD EALEKYGKAL PDETLEACRK 

        70         80         90        100        110        120 
SDAVLFGAAG ETAADVIVRL RRELGTFANV RPAKAIEGIE CLYPGLDIVV VRENTECLYM 

       130        140        150        160        170        180 
GFEFGFGDVT EAIRVITREA SERIARYAFE LAKREGRKKV TALHKANVMK KTCGLFRDVC 

       190        200        210        220        230        240 
REVAKDYPEI QYNDYYIDAA CMYLVMDPFR FDVIVTTNMF GDIVSDLAAG LVGGLGLAPS 

       250        260        270        280        290        300 
ANVGERTAIF EPVHGAAFDI AGKGIANPTA MILTACMMLR HFGYVEEAKK VEEAVEKTIK 

       310        320 
EGKKTPDLGG NLKTMEFANE VASLLD 

O29627 in FASTA format

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