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UniProtKB/Swiss-Prot entry O23051


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name KAO1_ARATH
Primary accession number O23051
Secondary accession number Q9C5Y3
Integrated into Swiss-Prot on December 15, 1998
Sequence was last modified on January 1, 1998 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 64)
Name and origin of the protein
Protein name Ent-kaurenoic acid oxidase 1
Synonyms AtKAO1
EC 1.14.13.79
Cytochrome P450 88A3
Gene name
Name: KAO1
Synonyms: CYP88A3
OrderedLocusNames: At1g05160
ORFNames: YUP8H12.23
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME ACTIVITY, AND TISSUE SPECIFICITY.
STRAIN=cv. Landsberg erecta;
TISSUE=Silique;
DOI=10.1073/pnas.041588998; PubMed=11172076 [NCBI, ExPASy, EBI, Israel, Japan]
Helliwell C.A., Chandler P.M., Poole A., Dennis E.S., Peacock J.W.;
"The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway.";
Proc. Natl. Acad. Sci. U.S.A. 98:2065-2070(2001).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/35048500; PubMed=11130712 [NCBI, ExPASy, EBI, Israel, Japan]
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
Nature 408:816-820(2000).
[3]
SUBCELLULAR LOCATION.
PubMed=11722763 [NCBI, ExPASy, EBI, Israel, Japan]
Helliwell C.A., Sullivan J.A., Mould R.M., Gray J.C., Peacock W.J., Dennis E.S.;
"A plastid envelope location of Arabidopsis ent-kaurene oxidase links the plastid and endoplasmic reticulum steps of the gibberellin biosynthesis pathway.";
Plant J. 28:201-208(2001).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF318500; AAK11564.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC000098; AAB71462.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR H86185; H86185.
RefSeq NP_172008.1; -.
UniGene At.10414
3D structure databases
HSSP P14779; 1JPZ. [HSSP ENTRY / PDB]
ModBase O23051.
Enzyme and pathway databases
BioCyc MetaCyc:AT1G05160-MON; -.
Organism-specific databases
GeneFarm 1616; 94.
TAIR At1g05160; -.
Gene expression databases
ArrayExpress O23051; -.
GermOnline AT1G05160; Arabidopsis thaliana.
Ontologies
GO
GO:0005789; Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.
Family and domain databases
InterPro IPR001128; Cyt_P450.
IPR002397; Cyt_P450_B.
Graphical view of domain structure.
Gene3D G3DSA:1.10.630.10; Cyt_P450; 1.
PANTHER PTHR19383; Cyt_P450; 1.
Pfam PF00067; p450; 1.
Pfam graphical view of domain structure.
PRINTS PR00359; BP450.
PR00385; P450.
PROSITE PS00086; CYTOCHROME_P450; 1.
BLOCKS O23051.
Genome annotation databases
GeneID 839311; -.
GenomeReviews CT485782_GR; AT1G05160.
KEGG ath:AT1G05160; -.
Other
ProtoNet O23051.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Monooxygenase; NADP; Oxidoreductase; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   490  490     Ent-kaurenoic acid oxidase 1. PRO_0000052178
TRANSMEM   6    26  21     Potential. 
METAL   439   439        Iron (heme axial ligand) (By similarity). 
Sequence information
Length: 490 AA [This is the length of the unprocessed precursor] Molecular weight: 56409 Da [This is the MW of the unprocessed precursor] CRC64: 7FD8CD7A8864D298 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAETTSWIPV WFPLMVLGCF GLNWLVRKVN VWLYESSLGE NRHYLPPGDL GWPFIGNMLS 

        70         80         90        100        110        120 
FLRAFKTSDP DSFTRTLIKR YGPKGIYKAH MFGNPSIIVT TSDTCRRVLT DDDAFKPGWP 

       130        140        150        160        170        180 
TSTMELIGRK SFVGISFEEH KRLRRLTAAP VNGHEALSTY IPYIEENVIT VLDKWTKMGE 

       190        200        210        220        230        240 
FEFLTHLRKL TFRIIMYIFL SSESENVMDA LEREYTALNY GVRAMAVNIP GFAYHRALKA 

       250        260        270        280        290        300 
RKTLVAAFQS IVTERRNQRK QNILSNKKDM LDNLLNVKDE DGKTLDDEEI IDVLLMYLNA 

       310        320        330        340        350        360 
GHESSGHTIM WATVFLQEHP EVLQRAKAEQ EMILKSRPEG QKGLSLKETR KMEFLSQVVD 

       370        380        390        400        410        420 
ETLRVITFSL TAFREAKTDV EMNGYLIPKG WKVLTWFRDV HIDPEVFPDP RKFDPARWDN 

       430        440        450        460        470        480 
GFVPKAGAFL PFGAGSHLCP GNDLAKLEIS IFLHHFLLKY QVKRSNPECP VMYLPHTRPT 

       490 
DNCLARISYQ 

O23051 in FASTA format

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