[1]	NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION. TISSUE=Embryonic brain; DOI=10.1016/S0092-8674(00)81795-X; PubMed=8978605 [NCBI, ExPASy, EBI, Israel, Japan] Serafini T., Colamarino S.A., Leonardo E.D., Wang H., Beddington R., Skarnes W.C., Tessier-Lavigne M.; "Netrin-1 is required for commissural axon guidance in the developing vertebrate nervous system."; Cell 87:1001-1014(1996).
[2]	NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY. DOI=10.1016/S0925-4773(99)00035-0; PubMed=10381568 [NCBI, ExPASy, EBI, Israel, Japan] Pueschel A.W.; "Divergent properties of mouse netrins."; Mech. Dev. 83:65-75(1999).
[3]	NUCLEOTIDE SEQUENCE [MRNA] OF 352-398, AND SUBCELLULAR LOCATION. DOI=10.1073/pnas.92.14.6592; PubMed=7604039 [NCBI, ExPASy, EBI, Israel, Japan] Skarnes W.C., Moss J.E., Hurtley S.M., Beddington R.S.; "Capturing genes encoding membrane and secreted proteins important for mouse development."; Proc. Natl. Acad. Sci. U.S.A. 92:6592-6596(1995).

Comments

FUNCTION: Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis (By similarity).
SUBUNIT: Binds to its receptors; DCC, UNC5A, UNC5B, UNC5C and probably UNC5D (By similarity).
INTERACTION:
P70211:Dcc; NbExp=1; IntAct=EBI-1798844, EBI-1798863;
Q9ERC8:Dscam; NbExp=1; IntAct=EBI-1798844, EBI-1798601;
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
TISSUE SPECIFICITY: In the embryo, widely expressed in the developing nervous system and in mesodermal tissues.
SIMILARITY: Contains 3 laminin EGF-like domains.
SIMILARITY: Contains 1 laminin N-terminal domain.
SIMILARITY: Contains 1 NTR domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U65418; AAC52971.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF128865; AAD28602.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U23505; AAA87938.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

IPI00114257; -.

NP_032770.2; -.

3D structure databases

HSSP

P02468; 1NPE. [HSSP ENTRY / PDB]

ModBase

O09118.

Protein-protein interaction databases

IntAct

O09118; 2.

PTM databases

PhosphoSite

O09118; -.

Organism-specific databases

MGI

MGI:105088; Ntn1.

Gene expression databases

O09118; -.

O09118; -.

MM_NTN1; -.

ENSMUSG00000020902; Mus musculus.

Ontologies

GO:0005604;	Cellular component: basement membrane (inferred from direct assay from MGI).
GO:0005737;	Cellular component: cytoplasm (inferred from direct assay from MGI).
GO:0005615;	Cellular component: extracellular space (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005515;	Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0006915;	Biological process: apoptosis (inferred from electronic annotation from UniProtKB-KW).
GO:0007411;	Biological process: axon guidance (inferred from direct assay from MGI).
GO:0040023;	Biological process: establishment of nucleus localization (inferred from direct assay from MGI).
GO:0030879;	Biological process: mammary gland development (inferred from genetic interaction from MGI).
GO:0030517;	Biological process: negative regulation of axon extension (inferred from direct assay from MGI).
GO:0001764;	Biological process: neuron migration (inferred from mutant phenotype from MGI).
GO:0045773;	Biological process: positive regulation of axon extension (inferred from direct assay from MGI).
GO:0008284;	Biological process: positive regulation of cell proliferation (inferred from mutant phenotype from MGI).
GO:0030334;	Biological process: regulation of cell migration (inferred from direct assay from MGI).
QuickGo view.

Family and domain databases

InterPro

IPR013032; EGF-like_reg_CS.
IPR002049; EGF_laminin.
IPR008211; Laminin_N.
IPR001134; Netrin_domain.
IPR018933; Netrin_module_non-TIMP.
Graphical view of domain structure.

Pfam

PF00053; Laminin_EGF; 3.
PF00055; Laminin_N; 1.
PF01759; NTR; 1.
Pfam graphical view of domain structure.

SMART

SM00643; C345C; 1.
SM00180; EGF_Lam; 3.
SM00136; LamNT; 1.
SMART graphical view of domain structure.

PROSITE

PS00022; EGF_1; 2.
PS01186; EGF_2; FALSE_NEG.
PS01248; EGF_LAM_1; 3.
PS50027; EGF_LAM_2; 3.
PS51117; LAMININ_NTER; 1.
PS50189; NTR; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

Ensembl

ENSMUSG00000020902; Mus musculus. [Contig view]

GeneID

18208; -.

KEGG

mmu:18208; -.

Phylogenomic databases

HOGENOM

O09118; -.

HOVERGEN

O09118; -.

Other

293590; -.

Ntn1; Mus musculus.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Apoptosis; Disulfide bond; Extracellular matrix; Glycoprotein; Laminin EGF-like domain; Repeat; Secreted; Signal.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 24`	`24`	`Potential.`
`CHAIN`	`25 604`	`580`	`Netrin-1.`	`PRO_0000017083`
`DOMAIN`	`47 284`	`238`	`Laminin N-terminal.`
`DOMAIN`	`285 340`	`56`	`Laminin EGF-like 1.`
`DOMAIN`	`341 403`	`63`	`Laminin EGF-like 2.`
`DOMAIN`	`404 453`	`50`	`Laminin EGF-like 3.`
`DOMAIN`	`472 601`	`130`	`NTR.`
`MOTIF`	`530 532`	`3`	`Cell attachment site (Potential).`
`CARBOHYD`	`95 95`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`116 116`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`131 131`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`417 417`		`N-linked (GlcNAc...) (Potential).`
`DISULFID`	`119 152`		`By similarity.`
`DISULFID`	`285 294`		`By similarity.`
`DISULFID`	`287 304`		`By similarity.`
`DISULFID`	`306 315`		`By similarity.`
`DISULFID`	`318 338`		`By similarity.`
`DISULFID`	`341 350`		`By similarity.`
`DISULFID`	`343 368`		`By similarity.`
`DISULFID`	`371 380`		`By similarity.`
`DISULFID`	`383 401`		`By similarity.`
`DISULFID`	`404 416`		`By similarity.`
`DISULFID`	`406 423`		`By similarity.`
`DISULFID`	`425 434`		`By similarity.`
`DISULFID`	`437 451`		`By similarity.`
`DISULFID`	`472 544`		`By similarity.`
`DISULFID`	`491 601`		`By similarity.`

Sequence information

Length: 604 AA [This is the length of the unprocessed precursor]

Molecular weight: 67768 Da [This is the MW of the unprocessed precursor]

CRC64: 0BE67AE72B837313 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MMRAVWEALA ALAAVACLVG AVRGGPGLSM FAGQAAQPDP CSDENGHPRR CIPDFVNAAF 

        70         80         90        100        110        120 
GKDVRVSSTC GRPPARYCVV SERGEERVRS CHLCNSSDPK KAHPPAFLTD LNNPHNLTCW 

       130        140        150        160        170        180 
QSENYLQFPH NVTLTLSLGK KFEVTYVSLQ FCSPRPESMA IYKSMDYGRT WVPFQFYSTQ 

       190        200        210        220        230        240 
CRKMYNRPHR APITKQNEQE AVCTDSHTDM RPLSGGLIAF STLDGRPSAH DFDNSPVLQD 

       250        260        270        280        290        300 
WVTATDIRVA FSRLHTFGDE NEDDSELARD SYYYAVSDLQ VGGRCKCNGH AARCVRDRDD 

       310        320        330        340        350        360 
SLVCDCRHNT AGPECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC RFNMELYKLS 

       370        380        390        400        410        420 
GRKSGGVCLN CRHNTAGRHC HYCKEGFYRD MGKPITHRKA CKACDCHPVG AAGKTCNQTT 

       430        440        450        460        470        480 
GQCPCKDGVT GITCNRCAKG YQQSRSPIAP CIKIPVAPPT TAASSAEEPE DCDSYCKASK 

       490        500        510        520        530        540 
GKLKMNMKKY CRKDYAVQIH ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR 

       550        560        570        580        590        600 
DIACKCPKIK PLKKYLLLGN AEDSPDQSGI VADKSSLVIQ WRDTWARRLR KFQQREKKGK 


CKKA

O09118 in FASTA format

View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools