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UniProtKB/Swiss-Prot entry O05651


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PORA_THEMA
Primary accession number O05651
Secondary accession numbers None
Integrated into Swiss-Prot on December 15, 1998
Sequence was last modified on August 1, 1998 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 55)
Name and origin of the protein
Protein name Pyruvate synthase subunit porA
Synonyms EC 1.2.7.1
Pyruvate oxidoreductase alpha chain
POR
Pyruvic-ferredoxin oxidoreductase subunit alpha
Gene name
Name: porA
OrderedLocusNames: TM_0017
From
Thermotoga maritima [TaxID: 2336] [HAMAP proteome]
Taxonomy Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-43.
STRAIN=ATCC 43589 / MSB8 / DSM 3109 / JCM 10099;
PubMed=8550425 [NCBI, ExPASy, EBI, Israel, Japan]
Kletzin A., Adams M.W.W.;
"Molecular and phylogenetic characterization of pyruvate and 2-ketoisovalerate ferredoxin oxidoreductases from Pyrococcus furiosus and pyruvate ferredoxin oxidoreductase from Thermotoga maritima.";
J. Bacteriol. 178:248-257(1996).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 43589 / MSB8 / DSM 3109 / JCM 10099;
DOI=10.1038/20601; PubMed=10360571 [NCBI, ExPASy, EBI, Israel, Japan]
Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M.;
"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima.";
Nature 399:323-329(1999).
[3]
PROTEIN SEQUENCE OF 1-43, AND CHARACTERIZATION.
STRAIN=ATCC 43589 / MSB8 / DSM 3109 / JCM 10099;
DOI=10.1021/bi00170a019; PubMed=8305426 [NCBI, ExPASy, EBI, Israel, Japan]
Blamey J.M., Adams M.W.W.;
"Characterization of an ancestral type of pyruvate ferredoxin oxidoreductase from the hyperthermophilic bacterium, Thermotoga maritima.";
Biochemistry 33:1000-1007(1994).
Comments
  • CATALYTIC ACTIVITY: Pyruvate + CoA + 2 oxidized ferredoxin = acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+.
  • SUBUNIT: Heterotetramer of one alpha, one beta, one delta and one gamma chain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X85171; CAA59457.1; ALT_INIT; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE000512; AAD35111.1; ALT_INIT; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR D59427; C72427.
RefSeq NP_227833.1; -.
3D structure databases
HSSP P94692; 1KEK. [HSSP ENTRY / PDB]
ModBase O05651.
Enzyme and pathway databases
BioCyc MetaCyc:MON-423; -.
TMAR243274:TM_0017-MON; -.
Ontologies
GO
GO:0019164; Molecular function: pyruvate synthase activity (inferred from electronic annotation from EC).
QuickGo view.
Family and domain databases
InterPro IPR002880; Pyrv_Fd/Flavodoxin_OxRdtase_N.
IPR015941; Transketolase_C-like.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.920; Transketo_C_like; 1.
Pfam PF01855; POR_N; 1.
Pfam graphical view of domain structure.
BLOCKS O05651.
Genome annotation databases
GeneID 896831; -.
GenomeReviews AE000512_GR; TM_0017.
KEGG tma:TM0017; -.
NMPDR fig|243274.1.peg.17; -.
TIGR TM_0017; -.
Phylogenomic databases
HOGENOM O05651; -.
Other
ProtoNet O05651.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Direct protein sequencing; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom  To Length Description FTId
CHAIN   1   392  392     Pyruvate synthase subunit porA. PRO_0000099902
COMPBIAS   72    79  8     Poly-Ala. 
Sequence information
Length: 392 AA [This is the length of the unprocessed precursor] Molecular weight: 44112 Da [This is the MW of the unprocessed precursor] CRC64: ACEF0E34C9EC1818 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MERVVERVAV TGAEAVANAM RQIEPDVVAA YPITPQTPIV EYFARFVADG VVRTEMIPVE 

        70         80         90        100        110        120 
SEHSAMSAVV GAAAAGARAM TATSANGLAL MHEIVYIAAS YRLPIVMPVV NRALSGPINI 

       130        140        150        160        170        180 
HCDHSDAMAE RDSGWIQLFA ETNQEAYDFT ILAVRLAEHE DVRLPVMVNL DGFILSHGVE 

       190        200        210        220        230        240 
PVEFYPDELV KKFVGELKPM YPLLDTEHPV TWGPLDLYDY YFEHKRQQIE AMENVKKVFP 

       250        260        270        280        290        300 
EIAKEFEETF GRKYWFVEPY RMEDAEHVMV ALGSTNSTIK YVVDELREEG YKVGSLKIWM 

       310        320        330        340        350        360 
FRPFPKEQLQ ELLNGRKSVV VLDRAVSFGA EAPLYEAVKS ALYEVAARPM LGSYVYGLGG 

       370        380        390 
RDIKPEHIRK AFEDAINGNL IADEQRYLGL RE 

O05651 in FASTA format

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