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UniProtKB/Swiss-Prot entry O05151


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name BPHF_RHOSR
Primary accession number O05151
Secondary accession number Q75WN6
Integrated into Swiss-Prot on December 1, 2000
Sequence was last modified on July 1, 1997 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 42)
Name and origin of the protein
Protein name 4-hydroxy-2-oxovalerate aldolase
Synonyms HOA
EC 4.1.3.39
Gene name
Name: bphF
Synonyms: etbF
OrderedLocusNames: RHA1_ro10138
From
Rhodococcus sp. (strain RHA1) [TaxID: 101510] [HAMAP proteome]
Encoded on Plasmid pRHL2.
Taxonomy Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; Corynebacterineae; Nocardiaceae; Rhodococcus.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
DOI=10.1016/S0378-1119(96)00748-2; PubMed=9073078 [NCBI, ExPASy, EBI, Israel, Japan]
Masai E., Sugiyama K., Iwashita N., Shimizu S., Hauschild J.E., Hatta T., Kimbara K., Yano K., Fukuda M.;
"The bphDEF meta-cleavage pathway genes involved in biphenyl/polychlorinated biphenyl degradation are located on a linear plasmid and separated from the initial bphACB genes in Rhodococcus sp. strain RHA1.";
Gene 187:141-149(1997).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Iwasaki T., Miyauchi K., Masai E., Fukuda M.;
"Diversity and characterization of aromatic ring hydroxylation dioxygenase genes in Rhodococcus sp. strain RHA1.";
Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1073/pnas.0607048103; PubMed=17030794 [NCBI, ExPASy, EBI, Israel, Japan]
McLeod M.P., Warren R.L., Hsiao W.W.L., Araki N., Myhre M., Fernandes C., Miyazawa D., Wong W., Lillquist A.L., Wang D., Dosanjh M., Hara H., Petrescu A., Morin R.D., Yang G., Stott J.M., Schein J.E., Shin H., Smailus D., Siddiqui A.S., Marra M.A., Jones S.J.M., Holt R., Brinkman F.S.L., Miyauchi K., Fukuda M., Davies J.E., Mohn W.W., Eltis L.D.;
"The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse.";
Proc. Natl. Acad. Sci. U.S.A. 103:15582-15587(2006).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
D78322; BAA18937.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB120955; BAC92717.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CP000433; ABH00331.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR JC6327; JC6327.
RefSeq YP_708489.1; -.
3D structure databases
HSSP P23522; 1DXE. [HSSP ENTRY / PDB]
ModBase O05151.
Enzyme and pathway databases
BioCyc RSP101510:RHA1_RO10138-MON; -.
Ontologies
GO
GO:0008701; Molecular function: 4-hydroxy-2-oxovalerate aldolase activity (inferred from electronic annotation from EC).
GO:0019439; Biological process: aromatic compound catabolic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR005000; HpcH_HpaI.
IPR015813; Pyrv/PenolPyrv_Kinase_cat.
Graphical view of domain structure.
Gene3D G3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 1.
Pfam PF03328; HpcH_HpaI; 1.
Pfam graphical view of domain structure.
BLOCKS O05151.
ProtoNet O05151.
Genome annotation databases
GeneID 4226121; -.
GenomeReviews CP000433_GR; RHA1_ro10138.
KEGG rha:RHA1_ro10138; -.
Phylogenomic databases
HOGENOM O05151; -.
Genome annotation databases
CMR O05151; RHA1_ro10138.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Aromatic hydrocarbons catabolism; Complete proteome; Lyase; Magnesium; Metal-binding; Plasmid.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   258  258     4-hydroxy-2-oxovalerate aldolase. PRO_0000207097
ACT_SITE   48    48        Proton acceptor (By similarity). 
METAL   151   151        Magnesium (By similarity). 
METAL   177   177        Magnesium (By similarity). 
BINDING   149   149        Substrate (By similarity). 
BINDING   176   176        Substrate; via amide nitrogen (By similarity). 
BINDING   177   177        Substrate (By similarity). 
SITE   74    74  1     Transition state stabilizer (By similarity). 
SITE   88    88  1     Increases basicity of active site His (By similarity). 
Sequence information
Length: 258 AA [This is the length of the unprocessed precursor] Molecular weight: 27159 Da [This is the MW of the unprocessed precursor] CRC64: A539C46C4DA0190F [This is a checksum on the sequence]
        10         20         30         40         50         60 
MQSPINSFKK ALAEGRTQIG FWLALGDAYS AEVCAGAGFD WLLIDGEHAP QDLRSVLAQL 

        70         80         90        100        110        120 
QVIGAYRDCH AAVRVPSADT TVIKQYLDLG AQSLLVPMVD TADEAAAVVR ACRYPPGGIR 

       130        140        150        160        170        180 
GVGGARASRW GRYPRYLHEA DEQVCVVVQA ETALALSNLE AIAEVDGIDG VFIGTADLAA 

       190        200        210        220        230        240 
SLGFPGNPAH PEVQDAILDA LQRVRAAGKA PGVLTPVEDL AQKYLAHGAV FVAVGIDTHL 

       250 
LAKQTSALAA RFAQVAYS 

O05151 in FASTA format

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