ID P5CS_ORYSJ Reviewed; 716 AA. AC O04226; Q60EM4; Q6PW76; DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot. DT 30-MAY-2006, sequence version 2. DT 25-NOV-2008, entry version 67. DE RecName: Full=Delta-1-pyrroline-5-carboxylate synthetase; DE Short=P5CS; DE Includes: DE RecName: Full=Glutamate 5-kinase; DE Short=GK; DE EC=2.7.2.11; DE AltName: Full=Gamma-glutamyl kinase; DE Includes: DE RecName: Full=Gamma-glutamyl phosphate reductase; DE Short=GPR; DE EC=1.2.1.41; DE AltName: Full=Glutamate-5-semialdehyde dehydrogenase; DE AltName: Full=Glutamyl-gamma-semialdehyde dehydrogenase; GN Name=P5CS; OrderedLocusNames=Os05g0455500, LOC_Os05g38150; GN ORFNames=OJ1651_D06.9; OS Oryza sativa subsp. japonica (Rice). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; OC Ehrhartoideae; Oryzeae; Oryza. OX NCBI_TaxID=39947; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=cv. Akibare; RX MEDLINE=97260389; PubMed=9106509; DOI=10.1023/A:1005702408601; RA Igarashi Y., Yoshiba Y., Sanada Y., Yamaguchi-Shinozaki K., Wada K., RA Shinozaki K.; RT "Characterization of the gene for delta1-pyrroline-5-carboxylate RT synthetase and correlation between the expression of the gene and salt RT tolerance in Oryza sativa L."; RL Plant Mol. Biol. 33:857-865(1997). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=cv. Tainung 71; RA Huang T.-C., Chuang H.-S., Yen T.-Y., Huang Y.-W., Wu M.-L.; RT "Nucleotide sequence of delta-1-pyrroline-5-carboxylate synthetase."; RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Nipponbare; RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y; RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., RA Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.; RT "A fine physical map of the rice chromosome 5."; RL Mol. Genet. Genomics 274:337-345(2005). CC -!- FUNCTION: P5CS plays a key role in proline biosynthesis, leading CC to osmoregulation in plants. CC -!- CATALYTIC ACTIVITY: ATP + L-glutamate = ADP + L-glutamate 5- CC phosphate. CC -!- CATALYTIC ACTIVITY: L-glutamate 5-semialdehyde + phosphate + CC NADP(+) = L-glutamyl 5-phosphate + NADPH. CC -!- ENZYME REGULATION: Feedback regulated by proline. CC -!- PATHWAY: Amino-acid biosynthesis; L-proline biosynthesis; L- CC glutamate 5-semialdehyde from L-glutamate: step 1/2. CC -!- PATHWAY: Amino-acid biosynthesis; L-proline biosynthesis; L- CC glutamate 5-semialdehyde from L-glutamate: step 2/2. CC -!- TISSUE SPECIFICITY: Expressed at high levels in leaves and is CC inducible in roots subjected to salt stress. CC -!- SIMILARITY: In the N-terminal section; belongs to the glutamate 5- CC kinase family. CC -!- SIMILARITY: In the C-terminal section; belongs to the gamma- CC glutamyl phosphate reductase family. CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution-NoDerivs License CC ----------------------------------------------------------------------- DR EMBL; D49714; BAA19916.1; -; mRNA. DR EMBL; AY574031; AAS89034.1; -; mRNA. DR EMBL; AC111016; AAU90213.1; -; Genomic_DNA. DR PIR; T03695; T03695. DR RefSeq; NP_001055723.1; -. DR UniGene; Os.12281; -. DR HSSP; Q9WYC9; 1O20. DR GeneID; 4338979; -. DR KEGG; osa:4338979; -. DR Gramene; O04226; -. DR GO; GO:0005737; C:cytoplasm; IEA:InterPro. DR GO; GO:0004349; F:glutamate 5-kinase activity; IEA:InterPro. DR GO; GO:0004350; F:glutamate-5-semialdehyde dehydrogenase acti...; IEA:InterPro. DR GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW. DR GO; GO:0006561; P:proline biosynthetic process; IEA:InterPro. DR InterPro; IPR016163; Ald_DHase_C. DR InterPro; IPR016162; Ald_DHase_N. DR InterPro; IPR001048; Asp/Glu/Uridylate_kinase. DR InterPro; IPR000965; Gglut_pp_reduct. DR InterPro; IPR001057; Glu_5kinase. DR InterPro; IPR005766; P5_carboxy_syn. DR InterPro; IPR005715; ProB. DR Gene3D; G3DSA:3.40.1160.10; Aa_kinase; 1. DR Gene3D; G3DSA:3.40.309.10; Aldehyde_dehydrogenase_C; 1. DR Gene3D; G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1. DR Pfam; PF00696; AA_kinase; 1. DR PIRSF; PIRSF036429; P5C_syn; 1. DR PRINTS; PR00474; GLU5KINASE. DR TIGRFAMs; TIGR01092; P5CS; 1. DR TIGRFAMs; TIGR00407; proA; 1. DR TIGRFAMs; TIGR01027; proB; 1. DR PROSITE; PS00902; GLUTAMATE_5_KINASE; 1. DR PROSITE; PS01223; PROA; 1. PE 2: Evidence at transcript level; KW Amino-acid biosynthesis; Kinase; Multifunctional enzyme; NADP; KW Oxidoreductase; Proline biosynthesis; Transferase. FT CHAIN 1 716 Delta-1-pyrroline-5-carboxylate FT synthetase. FT /FTId=PRO_0000109777. FT REGION 1 296 Glutamate 5-kinase. FT REGION 297 716 Gamma-glutamyl phosphate reductase. FT CONFLICT 173 173 F -> S (in Ref. 2; AAS89034). FT CONFLICT 488 488 I -> T (in Ref. 1; BAA19916). FT CONFLICT 500 500 K -> E (in Ref. 2; AAS89034). FT CONFLICT 530 530 H -> L (in Ref. 1; BAA19916). FT CONFLICT 537 537 I -> T (in Ref. 1; BAA19916). SQ SEQUENCE 716 AA; 77745 MW; F8D3C7D9449CFA64 CRC64; MASVDPSRSF VRDVKRVIIK VGTAVVSRQD GRLALGRVGA LCEQVKELNS LGYEVILVTS GAVGVGRQRL RYRKLVNSSF ADLQKPQMEL DGKACAAVGQ SGLMALYDML FNQLDVSSSQ LLVTDSDFEN PKFREQLTET VESLLDLKVI PIFNENDAIS TRKAPYEDSS GIFWDNDSLA GLLALELKAD LLILLSDVDG LYSGPPSEPS SKIIHTYIKE KHQQEITFGD KSRVGRGGMT AKVKAAVLAS NSGTPVVITS GFENRSILKV LHGEKIGTLF HKNANLWESS KDVSTREMAV AARDCSRHLQ NLSSEERKKI LLDVADALEA NEDLIRSENE ADVAAAQVAG YEKPLVARLT IKPGKIASLA KSIRTLANME DPINQILKKT EVADDLVLEK TSCPLGVLLI VFESRPDALV QIASLAIRSG NGLLLKGGKE AIRSNTILHK VITDAIPRNV GEKLIGLVTT RDEIADLLKL DDVIDLVIPR GSNKLVSQIK ASTKIPVLGH ADGICHVYID KSADMDMAKH IVMDAKIDYP AACNAMETLL VHKDLMKSPG LDDILVALKT EGVNIYGGPI AHKALGFPKA VSFHHEYSSM ACTVEFVDDV QSAIDHIHRY GSAHTDCIVT TDDKVAETFL RRVDSAAVFH NASTRFSDGA RFGLGAEVGI STGRIHARGP VGVEGLLTTR WILRGRGQVV NGDKDVVYTH KSLPLQ //