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UniProtKB/Swiss-Prot entry O02373


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name UGDH_DROME
Primary accession number O02373
Secondary accession numbers O02647 Q9VRZ9
Integrated into Swiss-Prot on December 15, 1998
Sequence was last modified on July 1, 1997 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 71)
Name and origin of the protein
Protein name UDP-glucose 6-dehydrogenase
Synonyms UDP-Glc dehydrogenase
UDP-GlcDH
UDPGDH
EC 1.1.1.22
Protein sugarless
Gene name
Name: sgl
Synonyms: kiwi, ska
ORFNames: CG10072
From
Drosophila melanogaster (Fruit fly) [TaxID: 7227] 
Taxonomy Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=9217004 [NCBI, ExPASy, EBI, Israel, Japan]
Binari R.C., Staveley B.E., Johnson W.A., Godavarti R., Sasisekharan R., Manoukian A.S.;
"Genetic evidence that heparin-like glycosaminoglycans are involved in wingless signaling.";
Development 124:2623-2632(1997).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=9342049 [NCBI, ExPASy, EBI, Israel, Japan]
Haecker U., Lin X., Perrimon N.;
"The Drosophila sugarless gene modulates Wingless signaling and encodes an enzyme involved in polysaccharide biosynthesis.";
Development 124:3565-3573(1997).
[3]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=9272947 [NCBI, ExPASy, EBI, Israel, Japan]
Haerry T.E., Heslip T.R., Marsh J.L., O'Connor M.B.;
"Defects in glucuronate biosynthesis disrupt Wingless signaling in Drosophila.";
Development 124:3055-3064(1997).
[4]
NUCLEOTIDE SEQUENCE.
STRAIN=Canton-S;
Benevolenskaya E.V., Frolov M.V., Birchler J.A.;
"Mutation in the Drosophila gene encoding UDP-glucose dehydrogenase affects expression of unlinked genes.";
Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Berkeley;
DOI=10.1126/science.287.5461.2185; PubMed=10731132 [NCBI, ExPASy, EBI, Israel, Japan]
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
"The genome sequence of Drosophila melanogaster.";
Science 287:2185-2195(2000).
[6]
GENOME REANNOTATION.
PubMed=12537572 [NCBI, ExPASy, EBI, Israel, Japan]
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.";
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
[7]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=Berkeley;
TISSUE=Embryo;
PubMed=12537569 [NCBI, ExPASy, EBI, Israel, Japan]
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.;
"A Drosophila full-length cDNA resource.";
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF007870; AAB63208.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF000570; AAB58714.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF009013; AAB63462.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF001310; AAC97125.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF001312; AAC97126.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF001311; AAC97126.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE014296; AAF50631.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY052137; AAK93561.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_476980.1; -.
UniGene Dm.4371
3D structure databases
ModBase O02373.
Enzyme and pathway databases
BioCyc DMEL-XXX-02:DMEL-XXX-02-015497-MON; -.
Organism-specific databases
FlyBase FBgn0010851; sgl.
Gene expression databases
ArrayExpress O02373; -.
GermOnline CG10072; Drosophila melanogaster.
Ontologies
GO
GO:0003979; Molecular function: UDP-glucose 6-dehydrogenase activity (inferred from mutant phenotype from UniProtKB).
GO:0030206; Biological process: chondroitin sulfate biosynthetic process (inferred from mutant phenotype from UniProtKB).
GO:0007427; Biological process: epithelial cell migration, open tracheal system (inferred from mutant phenotype from UniProtKB).
GO:0008543; Biological process: fibroblast growth factor receptor signaling pathway (inferred from mutant phenotype from UniProtKB).
GO:0007507; Biological process: heart development (non-traceable author statement from FlyBase).
GO:0015014; Biological process: heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process (inferred from mutant phenotype from UniProtKB).
GO:0007509; Biological process: mesoderm migration (inferred from mutant phenotype from UniProtKB).
GO:0007428; Biological process: primary branching, open tracheal system (traceable author statement from FlyBase).
GO:0007367; Biological process: segment polarity determination (inferred from mutant phenotype from FlyBase).
GO:0007224; Biological process: smoothened signaling pathway (traceable author statement from FlyBase).
GO:0016055; Biological process: Wnt receptor signaling pathway (traceable author statement from FlyBase).
QuickGo view.
Family and domain databases
InterPro IPR016040; NAD(P)-bd.
IPR017476; Nucleotide_sugar_DH.
IPR014027; UDP-Glc/GDP-Man_DHase_C.
IPR014026; UDP-Glc/GDP-Man_DHase_dimer.
IPR014028; UDP-Glc/GDP-Man_DHase_dimer-bd.
IPR001732; UDP-Glc/GDP-Man_DHase_N.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
G3DSA:3.40.50.1870; UDP-Glc/GDP-Man_DH_C; 1.
PANTHER PTHR11374; UDPG_MGDP_DH_Creg; 1.
Pfam PF00984; UDPG_MGDP_dh; 1.
PF03720; UDPG_MGDP_dh_C; 1.
PF03721; UDPG_MGDP_dh_N; 1.
Pfam graphical view of domain structure.
BLOCKS O02373.
Genome annotation databases
Ensembl CG10072; Drosophila melanogaster. [Contig view]
GeneID 38760; -.
KEGG dme:Dmel_CG10072; -.
NMPDR fig|7227.3.peg.8592; -.
Phylogenomic databases
HOGENOM O02373; -.
Other
ProtoNet O02373.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   476  476     UDP-glucose 6-dehydrogenase. PRO_0000074064
NP_BIND   2    19  18     NAD (By similarity). 
REGION   158   161  4     Substrate binding (By similarity). 
ACT_SITE   272   272        Nucleophile (By similarity). 
BINDING   32    32        NAD (By similarity). 
BINDING   37    37        NAD (By similarity). 
BINDING   89    89        NAD (By similarity). 
BINDING   127   127        NAD; via amide nitrogen (By similarity). 
BINDING   161   161        NAD (By similarity). 
BINDING   216   216        Substrate (By similarity). 
BINDING   269   269        Substrate; via amide nitrogen (By similarity). 
BINDING   275   275        NAD (By similarity). 
BINDING   335   335        Substrate (By similarity). 
BINDING   342   342        NAD (By similarity). 
CONFLICT   59    59        K -> R (in Ref. 3; AAC97125). 
Sequence information
Length: 476 AA [This is the length of the unprocessed precursor] Molecular weight: 52875 Da [This is the MW of the unprocessed precursor] CRC64: 1450B9A0C802BBE7 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKVCCIGAGY VGGPTCAVMA LKCPDIVITL VDKSSERIAQ WNSDKLPIYE PGLDEVVKKC 

        70         80         90        100        110        120 
RNVNLFFSTD IETAIKEADL IFISVNTPTK TCGNGKGRAA DLKYVESAAR MIAEIAQSNK 

       130        140        150        160        170        180 
IVVEKSTVPV RAAESIMHIL RANQKPGIHY DILSNPEFLA EGTAINDLLN ADRVLIGGEE 

       190        200        210        220        230        240 
TPEGHQAVEK LSWIYEHWIP KQNILTTNTW SSELSKLAAN AFLAQRISSI NSLSAVCEAT 

       250        260        270        280        290        300 
GADVSEVARA VGLDSRIGSK FLQASVGFGG SCFQKDILNL IYICENLNLP EVAAYWQQVI 

       310        320        330        340        350        360 
DMNEYQKRRF SQKIIESLFN TVSDKRIAIL GFAFKKNTGD TRETAAITVC QTLLEEGAAL 

       370        380        390        400        410        420 
DIYDPKVEPE QIIDDLTHPS VTESPEKVKK AVQIHSDPYS AVRATHALVI CTEWDEFVDL 

       430        440        450        460        470 
DFKRIYQSMM KPAYIFDGRK ILDHERLQQI GFHVQTIGKK YQRTGLLRSW GIVPQL 

O02373 in FASTA format

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