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UniProtKB/Swiss-Prot entry O00487


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PSDE_HUMAN
Primary accession number O00487
Secondary accession number O00176
Integrated into Swiss-Prot on August 31, 2004
Sequence was last modified on July 1, 1997 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 65)
Name and origin of the protein
Protein name 26S proteasome non-ATPase regulatory subunit 14
Synonyms 26S proteasome regulatory subunit rpn11
26S proteasome-associated PAD1 homolog 1
Gene name
Name: PSMD14
Synonyms: POH1
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
TISSUE=Lung;
DOI=10.1074/jbc.272.48.30470; PubMed=9374539 [NCBI, ExPASy, EBI, Israel, Japan]
Spataro V., Toda T., Craig R., Seeger M., Dubiel W., Harris A.L., Norbury C.;
"Resistance to diverse drugs and ultraviolet light conferred by overexpression of a novel human 26 S proteasome subunit.";
J. Biol. Chem. 272:30470-30475(1997).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Testis;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[3]
PROTEIN SEQUENCE OF 199-208, AND MASS SPECTROMETRY.
TISSUE=Brain, and Cajal-Retzius cell;
Lubec G., Afjehi-Sadat L.;
Submitted (MAR-2007) to UniProtKB.
[4]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-32, AND MASS SPECTROMETRY.
DOI=10.1038/nbt1046; PubMed=15592455 [NCBI, ExPASy, EBI, Israel, Japan]
Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.;
"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.";
Nat. Biotechnol. 23:94-101(2005).
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-224, AND MASS SPECTROMETRY.
DOI=10.1021/bi061994u; PubMed=17323924 [NCBI, ExPASy, EBI, Israel, Japan]
Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L.;
"Mass spectrometric characterization of the affinity-purified human 26S proteasome complex.";
Biochemistry 46:3553-3565(2007).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-150, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700120-MCP200; PubMed=17693683 [NCBI, ExPASy, EBI, Israel, Japan]
Tang L.-Y., Deng N., Wang L.-S., Dai J., Wang Z.-L., Jiang X.-S., Li S.-J., Li L., Sheng Q.-H., Wu D.-Q., Li L., Zeng R.;
"Quantitative phosphoproteome profiling of Wnt3a-mediated signaling network: indicating the involvement of ribonucleoside-diphosphate reductase M2 subunit phosphorylation at residue serine 20 in canonical Wnt signal transduction.";
Mol. Cell. Proteomics 6:1952-1967(2007).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U86782; AAC51866.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC066336; AAH66336.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_005796.1; -.
UniGene Hs.567410
3D structure databases
ModBase O00487.
Protein-protein interaction databases
IntAct O00487; -.
Protein family/group databases
MEROPS M67.001; -.
PTM databases
PhosphoSite O00487; -.
Enzyme and pathway databases
Reactome REACT_11045; Signaling by Wnt.
REACT_152; Cell Cycle, Mitotic.
REACT_1538; Cell Cycle Checkpoints.
REACT_383; DNA Replication.
REACT_6185; HIV Infection.
REACT_6850; Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_9035; APC/C:Cdh1-mediated degradation of Skp2.
2D gel databases
OGP O00487; -.
REPRODUCTION-2DPAGE IPI00024821; -.
Organism-specific databases
HGNC HGNC:16889; PSMD14.
GenAtlas PSMD14.
HPA HPA002114; -.
MIM 607173; gene. [NCBI / EBI]
PharmGKB PA134957776; -.
GeneCards O00487.
Gene expression databases
ArrayExpress O00487; -.
CleanEx HS_PSMD14; -.
GermOnline ENSG00000115233; Homo sapiens.
Ontologies
GO
GO:0005829; Cellular component: cytosol (inferred from electronic annotation from UniProtKB-KW).
GO:0005634; Cellular component: nucleus (inferred from direct assay from HPA).
GO:0000502; Cellular component: proteasome complex (traceable author statement from ProtInc).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0031145; Biological process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process (inferred from experiment from Reactome).
GO:0051436; Biological process: negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle (inferred from experiment from Reactome).
GO:0051437; Biological process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle (inferred from experiment from Reactome).
QuickGo view.
Family and domain databases
InterPro IPR000555; Mov34_MPN_PAD1.
Graphical view of domain structure.
Pfam PF01398; Mov34; 1.
Pfam graphical view of domain structure.
ProDom PD363422; Mov34-1; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00232; JAB_MPN; 1.
SMART graphical view of domain structure.
BLOCKS O00487.
ProtoNet O00487.
Proteomic databases
PeptideAtlas O00487; -.
Genome annotation databases
Ensembl ENSG00000115233; Homo sapiens. [Contig view]
GeneID 10213; -.
KEGG hsa:10213; -.
Phylogenomic databases
HOGENOM O00487; -.
HOVERGEN O00487; -.
Other
LinkHub O00487; -.
NextBio 38668; -.
SOURCE PSMD14; Homo sapiens.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Direct protein sequencing; Phosphoprotein; Proteasome.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   310  310     26S proteasome non-ATPase regulatory subunit 14. PRO_0000213952
DOMAIN   26   139  114     MPN. 
MOD_RES   32    32        Phosphotyrosine. 
MOD_RES   150   150        Phosphoserine. 
MOD_RES   224   224        Phosphoserine. 
Sequence information
Length: 310 AA [This is the length of the unprocessed precursor] Molecular weight: 34577 Da [This is the MW of the unprocessed precursor] CRC64: 18ACE876C7682039 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MDRLLRLGGG MPGLGQGPPT DAPAVDTAEQ VYISSLALLK MLKHGRAGVP MEVMGLMLGE 

        70         80         90        100        110        120 
FVDDYTVRVI DVFAMPQSGT GVSVEAVDPV FQAKMLDMLK QTGRPEMVVG WYHSHPGFGC 

       130        140        150        160        170        180 
WLSGVDINTQ QSFEALSERA VAVVVDPIQS VKGKVVIDAF RLINANMMVL GHEPRQTTSN 

       190        200        210        220        230        240 
LGHLNKPSIQ ALIHGLNRHY YSITINYRKN ELEQKMLLNL HKKSWMEGLT LQDYSEHCKH 

       250        260        270        280        290        300 
NESVVKEMLE LAKNYNKAVE EEDKMTPEQL AIKNVGKQDP KRHLEEHVDV LMTSNIVQCL 

       310 
AAMLDTVVFK 

O00487 in FASTA format

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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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