[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R.; Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.
CATALYTIC ACTIVITY: L-glutamate 5-semialdehyde + phosphate + NADP⁺ = L-glutamyl 5-phosphate + NADPH.
PATHWAY: Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2 .
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the gamma-glutamyl phosphate reductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000822; ABV14057.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001454493.1; -.

3D structure databases

ModBase

A8AKP4.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0004350;	Molecular function: glutamate-5-semialdehyde dehydrogenase activity (inferred from electronic annotation from HAMAP).
GO:0006561;	Biological process: proline biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00412; -; 1.
PBIL [Tree]

InterPro

IPR016163; Ald_DHase_C.
IPR016162; Ald_DHase_N.
IPR015590; Aldehyde_DHase.
IPR000965; Gglut_pp_reduct.
IPR012134; Glu-5-SA_DHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.309.10; Aldehyde_dehydrogenase_C; 1.
G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.

PANTHER

PTHR11063:SF1; GSA_DH; 1.

Pfam

PF00171; Aldedh; 1.
Pfam graphical view of domain structure.

PIRSF000151; GPR; 1.

TIGR00407; proA; 1.

PS01223; PROA; 1.

Genome annotation databases

GeneID

5581896; -.

GenomeReviews

CP000822_GR; CKO_02952.

KEGG

cko:CKO_02952; -.

CMR

A8AKP4; CKO_02952.

Other

ProtoNet

A8AKP4.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Amino-acid biosynthesis; Complete proteome; Cytoplasm; NADP; Oxidoreductase; Proline biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 417`	`417`		`Gamma-glutamyl phosphate reductase.`	`PRO_1000049943`

Sequence information

Length: 417 AA [This is the length of the unprocessed precursor]

Molecular weight: 44559 Da [This is the MW of the unprocessed precursor]

CRC64: B93B15BDEFE838D5 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLEQMGIAAK AASYKLALLS SREKNRVLEK IADELEAQTE SILSANAQDV AEARENGLSE 

        70         80         90        100        110        120 
AMLDRLALTP ARLKAIADDV RQVCNLADPV GQVIDGGLLD SGLRLERRRV PLGVIGVIYE 

       130        140        150        160        170        180 
ARPNVTVDVA SLCLKTGNAA ILRGGKETYR TNAATVAVIQ QALEACGLPA GAVQAIDSPD 

       190        200        210        220        230        240 
RALVNEMLRM DKYIDMLIPR GGAGLHKLCR EQSTIPVITG GIGVCHIFVD DSAEIEPALK 

       250        260        270        280        290        300 
IIVNAKTQRP STCNTVETLL VHQDIAARFL PALSKQMAES GVTLHADDAA LAQLKSGPAN 

       310        320        330        340        350        360 
VVAVKAEEYD DEFLSLDLNV KIVAGLDDAI AHIREHGTQH SDAILTRTLR NADRFVNEVD 

       370        380        390        400        410 
SSAVYVNAST RFTDGGQFGL GAEVAVSTQK LHARGPMGLE ALTTYKWIGI GDDTIRA

A8AKP4 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools