[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R.; Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (By similarity).
CATALYTIC ACTIVITY: [tRNA]-guanine + queuine = [tRNA]-queuine + guanine.
CATALYTIC ACTIVITY: [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine.
COFACTOR: Binds 1 zinc ion per subunit (By similarity).
PATHWAY: tRNA modification; queuosine-tRNA biosynthesis .
SIMILARITY: Belongs to the queuine tRNA-ribosyltransferase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000822; ABV13861.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001454297.1; -.

3D structure databases

ModBase

A8AK48.

Ontologies

GO:0008479;	Molecular function: queuine tRNA-ribosyltransferase activity (inferred from electronic annotation from HAMAP).
GO:0008270;	Molecular function: zinc ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008616;	Biological process: queuosine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00168; -; 1.
PBIL [Tree]

InterPro

IPR004803; QtRNA_ribo_trans.
IPR002616; tRNA_ribo_trans.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.105; tRNA_ribo_trans; 1.

PANTHER

PTHR11962; tRNA_ribo_trans; 1.

Pfam

PF01702; TGT; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00430; Q_tRNA_tgt; 1.
TIGR00449; tgt_general; 1.

ProtoNet

A8AK48.

Genome annotation databases

GeneID

5582299; -.

GenomeReviews

CP000822_GR; CKO_02755.

KEGG

cko:CKO_02755; -.

CMR

A8AK48; CKO_02755.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Glycosyltransferase; Metal-binding; Queuosine biosynthesis; Transferase; tRNA processing; Zinc.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 375`	`375`	`Queuine tRNA-ribosyltransferase.`	`PRO_1000016778`
`ACT_SITE`	`89 89`		`Nucleophile (By similarity).`
`METAL`	`302 302`		`Zinc (By similarity).`
`METAL`	`304 304`		`Zinc (By similarity).`
`METAL`	`307 307`		`Zinc (By similarity).`
`METAL`	`333 333`		`Zinc (By similarity).`
`BINDING`	`90 90`		`Substrate (By similarity).`

Sequence information

Length: 375 AA [This is the length of the unprocessed precursor]

Molecular weight: 42536 Da [This is the MW of the unprocessed precursor]

CRC64: A1A0B42EF3FBD057 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKFELDTTDG RARRGRLVFD RGVVETPAFM PVGTYGTVKG MTPEEVEATG AQIILGNTFH 

        70         80         90        100        110        120 
LWLRPGQEIM KLHGDLHDFM QWKGPILTDS GGFQVFSLGD IRKITEQGVH FRNPINGDPI 

       130        140        150        160        170        180 
FLDPEKSMEI QYDLGSDIVM IFDECTPYPA DWDYAKRSME MSLRWAKRSR DRFDGLGNKN 

       190        200        210        220        230        240 
ALFGIIQGSV YEDLRDISVK GLVEIGFDGY AVGGLAVGEP KEDMHRILEH VCPQIPADKP 

       250        260        270        280        290        300 
RYLMGVGKPE DLVEGVRRGI DMFDCVMPTR NARNGHLFVT DGVVKIRNAK HKSDTSPLDA 

       310        320        330        340        350        360 
ECDCYTCRNY SRAYLHHLDR CNEILGARLN TIHNLRYYQR LMAGLRKAIE EGKLESFVTD 

       370 
FYQRQGRPVP PLNVD

A8AK48 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools