[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=Hereford; TISSUE=Fetal skin; NIH - Mammalian Gene Collection (MGC) project; Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology (By similarity).
CATALYTIC ACTIVITY: A 3-oxo-5-alpha-steroid + acceptor = a 3-oxo-Delta⁴-steroid + reduced acceptor.
SUBCELLULAR LOCATION: Microsome membrane; Multi-pass membrane protein (By similarity). Endoplasmic reticulum membrane; Multi-pass membrane protein (Potential).
SIMILARITY: Belongs to the steroid 5-alpha reductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC142219; AAI42220.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001092607.1; -.

UniGene

Bt.27694

3D structure databases

ModBase

A5PJS2.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from InterPro).
GO:0005792;	Cellular component: microsome (inferred from electronic annotation from UniProtKB-KW).
GO:0003865;	Molecular function: 3-oxo-5-alpha-steroid 4-dehydrogenase activity (inferred from electronic annotation from InterPro).
GO:0030154;	Biological process: cell differentiation (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0007530;	Biological process: sex determination (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR016636; 3-oxo-5-alpha-steroid_4-DH.
IPR001104; 3-oxo-5_a-steroid_4-DHase_C.
Graphical view of domain structure.

Pfam

PF02544; Steroid_dh; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF015596; 5_alpha-SR2; 1.

PROSITE

PS50244; S5A_REDUCTASE; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

Ensembl

ENSBTAG00000015478; Bos taurus. [Contig view]

GeneID

614612; -.

KEGG

bta:614612; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Differentiation; Endoplasmic reticulum; Membrane; Microsome; Oxidoreductase; Sexual differentiation; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 257`	`257`	`3-oxo-5-alpha-steroid 4-dehydrogenase 1.`	`PRO_0000317710`
`TRANSMEM`	`7 27`	`21`	`Potential.`
`TRANSMEM`	`50 70`	`21`	`Potential.`
`TRANSMEM`	`84 104`	`21`	`Potential.`
`TRANSMEM`	`109 129`	`21`	`Potential.`
`TRANSMEM`	`149 169`	`21`	`Potential.`
`TRANSMEM`	`208 228`	`21`	`Potential.`

Sequence information

Length: 257 AA [This is the length of the unprocessed precursor]

Molecular weight: 29186 Da [This is the MW of the unprocessed precursor]

CRC64: 2A49937DFB45FFB0 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MELAERFLLD ALAYLECALG VVCYVLLKLV GSPYGRYASS GSAFGLPARA AWTVQELPSL 

        70         80         90        100        110        120 
ALPLLACAGA GAPAERLNRW PNCILLAMFL VHYAQRSLVF PFLIRGGKPM PLYAFLLAFI 

       130        140        150        160        170        180 
FCTYNGYLQS RYLSQYAVYA DDWLSDPRFL TGSALWLIGM LINIHSDHVL RNLRKPGETG 

       190        200        210        220        230        240 
YKIPRGGLFE YISAANYFGE VVEWCGYALA SWSIQGWAFA VFTFCVLFTR AQQHHKWYHE 

       250 
KFEDYPKFRK IMIPFLV

A5PJS2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools